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Citations for VWR :
1101 - 1150 of 1368 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 1:12 series of sections were quenched for 5 min using 10% H2O2 (VWR, West Sussex, UK) and methanol (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-30μg samples were prepared with a 6x reducing sample buffer containing β-mercaptoethanol (VWR, M131). Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% fetal bovine serum (Avantor, Radnor Township, PA) and 1% penicillin/streptomycin (VWR, Radnor, PA). Cultures were grown in an incubator set at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Larvae were cut perpendicular to the caudal notochord boundary using a surgical blade (Feather No. 10, VWR). Burn injury was performed on anesthetized larvae in a 60 mm tissue culture-treated dish containing 1x Tricaine with E3 ...
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bioRxiv - Physiology 2024Quote: ... 10 % Nipagin (SLS, FLY1136) in ethanol and an acid mixture comprised of 4.15 % phosphoric acid (VWR, 20624.262) and 41.8 % propionic acid (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were washed from 3 to 5 times for 10 min in PBS-Triton 0.8% (28817295, VWR) and permeabilized in 0.25% Trypsin-EDTA (sc391060 ...
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bioRxiv - Genomics 2024Quote: E15.5 mouse embryo FFPE sections (10 μm) were deparaffinized by immersion in the following reagent: Xylene (VWR) 15 minutes twice ...
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bioRxiv - Neuroscience 2024Quote: ... the overlay media was removed and cells were fixed/stained using 10% neutral buffered formalin (VWR #89370) and 0.25% crystal violet (VWR #0528 ...
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bioRxiv - Neuroscience 2024Quote: ... 150 μL of media was replaced with warmed DMEM/10% FBS media containing DMSO vehicle (VWR, WN182) or emetine (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... RAW 264.7 and J774A.1 cells were cultured in non-tissue culture-treated 10 cm dishes (VWR); all other cell lines were cultured in tissue culture- treated 10 cm dishes (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... One-day-old biofilms were grown on the surface of a 4-well Permanex slide (Lab-Tek® Chamber Slide™ System, VWR, Debrecen, Hungary). The preformed biofilms were washed three times with sterile physiological saline ...
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bioRxiv - Immunology 2024Quote: ... Tissues were dehydrated using an ascending methanol series and bleached overnight at 4 °C in a solution containing 5% H2O2 (VWR Chemicals, Radnor, PE, USA) in methanol ...
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bioRxiv - Cell Biology 2020Quote: ... dechorionated embryos were mounted on glue-coated coverslips (0.17mm, grade #1.5) and covered with Voltalef 10S oil (VWR). Movies of GFP::M6 accumulation were acquired using a 40x/1.3 NA oil immersion objective and the SP8 resonant scanner (line accumulation of 2 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 grams of cranberry powder was mixed with 100 mL of absolute ethanol (≥99.8%, VWR chemicals, AnalaR Normapur) under magnetic stirring for 30 minutes at 24±1°C sealed with parafilm ...
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bioRxiv - Bioengineering 2020Quote: ... SI was rolled using the Swiss-rolling technique and incubated in 10% Neutral Buffered Formalin (VWR, 10790-714) for 24 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS; Heat Inactivated Seradigm Premium Grade Fetal Bovine Serum, VWR, Radnor, PA) and 1 mM sodium pyruvate (Corning Inc. ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: Cell line MDA MB 436 was propagated in DMEM media supplemented with 10% FBS (VWR Seradigm Life Science), 1% L-glutamine and 1% sodium pyruvate (Life Technology-Thermo-Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 30 minutes in permeabilization buffer (PB; 0.5% Bovine serum albumin (VWR International; 126575-10), 0.25% Fish skin gelatin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 60-minute blocking with donkey serum (1:10 diluted in 1% BSA; VWR, Pennsylvania, USA). Incubation with the primary antibody was performed overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Mice (10-12 weeks) were suspended by their tails with adhesive tape (VWR or Fisher, 25 mm wide) to a flat metal bar connected to a force transducer that measures transduced movement ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... H2O up to a total volume of 10 μl was combined in a sealed short thread vial (VWR). For reducing condition freshly prepared DTT was added to a final concentration of 2 mM ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μm ventricular sections were cut using a Leica cryostat and attached to SuperFrost Plus™ slides (VWR). Slides were frozen at -20prior to immunostaining.
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bioRxiv - Biochemistry 2020Quote: ... 10 mM tetraoctylammonium bromide (TOAB) and solvent B contained 30 mM TOAB dissolved in HPLC grade acetonitrile (VWR). The column was subsequently cleaned for 10 mins using solvent B (isocratic ...
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bioRxiv - Pathology 2021Quote: ... The plate was centrifuged at 300 g for 10 min and fixed by directly adding paraformaldehyde (PFA, VWR) to the supernatant to a final concentration of 4% ...
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bioRxiv - Microbiology 2020Quote: ... Growth media were loaded into plastic 10 mL syringes (Codan) or glass vials (VWR, cat. no. 548-0154) and warmed to 37 °C at least 3 h prior to the start of each experiment.
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bioRxiv - Cell Biology 2021Quote: Embryoid bodies and monolayer cultures were fixed in 10% buffered formalin (Cat# CA71007-344, VWR, Radnor, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: α-Syn (1 mg/mL) was dissolved in 10 mM NaOH and sonicated (VWR® Symphony™ Ultrasonic Cleaner) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... whole livers were harvested from euthanized mice and placed in 10% buffered formalin (VWR Cat. No. 16004-128) in 50 mL conical tubes ...
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bioRxiv - Cell Biology 2023Quote: ... temperature-mediated antigen retrieval was performed in citrate buffer (10 mM citric acid monohydrate (VWR, 20276.235, Pennsylvania, USA), Ph 6 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with 1x PBS (137 mM NaCl, 2.7 mM KCl, and 10 mM PB, VWR) and then 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Biochemistry 2024Quote: ... and 10% glycerol with Cy3-Mono-Reactive Dye or Cy5-Mono-Reactive Dye overnight (VWR Catalog# 95017-379). The dye-labeled Cy3-RAD51 and Cy5-RAD51 was further purified as described above to remove any free dye in the protein prep ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with methanol for 5 seconds and stained with Giemsa (1:10 dilution with MiliQ, VWR) for 17 min ...
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bioRxiv - Developmental Biology 2022Quote: ... dechorionated embryos were mounted and lined up on a Menzel-Glaser coverslip with oil 10-S Voltalef (VWR) and covered with a membrane (YSI membrane kit) ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 min and blocked with 5% bovine serum albumin (VWR) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... Supernatant as diluted 1:10 in PBS and added to 384 well ELISA plates (VWR, cat. CA62409-064) that had been previously coated with 1 μg/mL NeutrAvidin (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min in 1x PBS and mounted onto SuperFrost slides (VWR, #48311- 703) using VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... for cryoprotection and allowed to sink at 4°C prior to being cryosectioned at 20 μm and placed on charged slides (VWR Superfrost® Plus, 48311-703).
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bioRxiv - Developmental Biology 2022Quote: ... dechorionated by hand and mounted on coverslips covered in heptane-dissolved glue and immersed in 10S Voltatef oil (VWR). All embryos were imaged between nc10 and nc14 at stable temperature (25°C ...
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bioRxiv - Neuroscience 2022Quote: ... mice were transcardially perfused with 1x PBS (137 mM NaCl, 2.7 mM KCl, and 10 mM phosphate buffer, VWR), followed by 4% paraformaldehyde ...
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bioRxiv - Neuroscience 2021Quote: Tissue samples were lysed in NP40 buffer (1% NP40 in 10 mM Tris, pH 7.4, 150 mM NaCl) in a Precellys homogenizator (VWR) and 10 μg total protein per sample were proteolyzed with Lys-C and trypsin using a modified FASP procedure (71).
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bioRxiv - Microbiology 2021Quote: ... We mixed 30 μl of each lysate with 200 μl UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 μl centrifugal filter unit (VWR International) and centrifuged the mixture at 14,000 x g for 40 min ...