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Citations for VWR :
1101 - 1150 of 1187 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Microbiology 2021Quote: L929 cells were grown in either suspension or monolayers in Joklik’s modified Eagle’s minimal essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085). CHO cells were grown in Ham’s F12 medium (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... after electrophoresis gels were immersed into a methanol/sodium acetate (pH=5) solution and incubated at room temperature on rocking platform (VWR) at 1 speed for 2 min with constant shaking ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Plant Biology 2023Quote: ... 4-day old Col_0 seedlings were incubated with LRC liquid medium supplemented with indicated concentrations of compounds (Adenosine 5′-triphosphate magnesium salt, ATP-Mg [VWR] ...
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bioRxiv - Bioengineering 2023Quote: ... the 3D printed PCL scaffolds were cut into dimensions of 5 mm × 15 mm using a surgical blade (VWR, Germany) and detached from the glass substrate by 70% EtOH (PanReac AppliChem ITW Reagents ...
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bioRxiv - Cancer Biology 2023Quote: ... purified cells/cell aggregates were washed in PBS and frozen in 5% dimethyl sulphoxide (DMSO; 23486.297, VWR, Radnor, PA, USA) in FBS until xenografting.
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... gels were immersed in a methanol-sodium acetate solution (pH 5) and incubated at room temperature on a rocking platform (VWR) at a speed of 1 for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were then irradiated with UV-A and UV-B for one hour a day for 5 consecutive days [42] in UV crosslinker (cat. no: 89131-486, VWR). On each exposure ...
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bioRxiv - Synthetic Biology 2023Quote: ... We grew the transformed library in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Neuroscience 2024Quote: ... larvae aged 5 dpf were transferred to individual wells of a 24-well plate at ZT7–8 (VWR, 82050-892), and acclimated overnight to the testing environment ...
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bioRxiv - Neuroscience 2024Quote: Frozen tissue was briefly thawed and mechanically lysed by addition of 100 μL lysis buffer (5% SDS, 50 mM Tris, pH 7.55 with HCl) and 10 ‘strokes’ with a pestle (VWR International) after which the pestle was washed with 100 μL additional lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Blots were washed with 1x TBS with 0.1% Tween-20 (TBST) and blocked using 5% IgG-free BSA (VWR RLBSA50). Primary antibodies (Supplemental Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... 200-300 million K-562 cells were collected by centrifugation (5 min at 200 x g, Mega Star 600 R centrifuge (VWR), rotor #75005701 (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The final nuclei pellet was resuspended in 100 ul of 10x Genomics Diluted Nuclei Buffer and trypan blue-stained nuclei were counted by eye using INCYTO C-Chip Neubauer Improved Disposable Hemacytometers (VWR International Ltd., Radnor, PA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The resulting pellets were resuspended in PBS and irradiated at 365 nm for 5 min (100 W lamp, VWR 36595-021) on ice ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
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bioRxiv - Microbiology 2020Quote: ... Cells were covered with Eagle’s Minimum Essential Medium (without phenol red, supplemented with 5% FBE, non-essential amino acids, 1mM sodium pyruvate (VWR, 45000-710), 2mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: A total of 5 × 106 MM.1Sgfp+/luc+ cells were seeded in T-25 flasks (Avantor/VWR, Cat. No. 10861-568) and treated with the vehicle (DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... one holes were bored into the lid of a Drosophila bottle and inserted a 5-μl glass capillaries (VWR, 53432-604) which filled with 20% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... we added another 0.5 ml PBS to the tubes that later were sonicated in the water bath of an ultrasonic cleaner (USC200TH, VWR International) for 5 min at 45 kHz ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and C were filled with 25 µl of 40 µM oligomycin for a final in-well concentration of 5 µM (“Oligo,” VWR Scientific), 4.5 µM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone for a final in-well concentration 0.5µM (“FCCP,” VWR Scientific) ...
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bioRxiv - Immunology 2023Quote: ... brains were isolated and placed into a 50 mL conical tube (Fisher Healthcare) containing 5 mL of RPMI (VWR International LLC). The RPMI containing the brains of the mice were then transferred to a 7 mL glass Tenbroeck dounce homogenizer (Pyrex ...
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bioRxiv - Developmental Biology 2023Quote: ... The 18 cryovials were tightly capped and placed in a 5 cm square cardboard cryobox that was immediately placed in a LN dewar (VWR CryoPro).
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed three times with 1X PBS (5 minutes each) and mounted on SuperFrost Plus micro slides (VWR 48311-703). Slices were covered with a 22 mm x 50 mm 1.5 thickness coverslip using ProLong Glass Antifade Mountant media (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... A 5 mg aliquot of each sample was subjected to extraction using methanol (HPLC - gradient grade, VWR International LLC, Randor, USA). The extraction process involved homogenization with 10 ceramic balls at a velocity of 6.5 m/s for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: Gastrocnemii were homogenized with 25 strokes using a 5 mL PTFE tissue grinder with clearance 0.15-0.25 mm (VWR 89026-392, 89026-404) at 3,000 rpm in RIPA lysis buffer (VWR 97063-270 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 50 ng/ml recombinant murine macrophage-colony stimulating factor (rmM-CSF, Genentech)] and plated in 15-cm non-TC treated dishes for 5 days (Petri dish, VWR, 25384-326). Fresh BMDM media was added on day 3 without removal of original media ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.4 μl of each primer and 5 μL VWR Red Taq DNA polymerase Master mix (VWR Chemicals, Denmark, Cas nr: 733-2547). The PCR reaction conditions for amplification of DNA were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...