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Citations for VWR :
1051 - 1100 of 1260 citations for 4 Triethylsilyl 3 butyn 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Bioengineering 2022Quote: ... Optical density (OD) was measured in technical triplicates in 1-mL polystyrene cuvettes in a V-1200 spectrophotometer (VWR) at 600 nm ...
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bioRxiv - Immunology 2022Quote: ... Cell density was adjusted to 1×106 cells/ml and added to the FACS tubes (Falcon® Brand, VWR). After pelleting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 1 mL of cold brain heart infusion (BHI) broth (VWR, cat. no. 90003-040) per infected T175 cm2 flask ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Microbiology 2023Quote: ... cells were scraped into 1 mL fresh KA medium using disposable cell scrapers with 20 mm blade width (VWR). Cells were pelleted with the respective supernatants discarded and then flash frozen in a dry ice-ethanol bath to preserve RNA integrity and stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 monocytes were differentiated into macrophages by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA, VWR) for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were diluted in 1 mL PBS and they or BALF were shaken in a glass vial (VWR) with 2 mL chloroform and 1 mL methanol containing internal standards (13C16-palmitic acid ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Neuroscience 2024Quote: ... S3: the lysate was extracted with an equal volume of phenol:chloroform:iso-amyl alcohol (25:24:1) (VWR, 97064-692), and the upper aqueous phase was then precipitated from ethanol.
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bioRxiv - Biochemistry 2024Quote: ... 500 µL of the upper phase was transferred in a new tube containing 1 mL isopropanol (VWR, 84881-320) and 2 µL f 15 mg/mL Glycoblue (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... adjusted for 1 hour in a 50:50 solution of 30% sucrose solution: Optimal Cutting Temperature (OCT) compound (VWR), followed by 1 hour in 100% OCT and snap-frozen in OCT using dry ice ...
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bioRxiv - Microbiology 2024Quote: 100 μl of cell suspension at OD 0.7 were added to 1 ml of fresh GCB medium in a 6-well plate (VWR) and incubated at 37 °C with 5% CO2 for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was immediately and gently mixed with 1 ml NCM medium containing 0.38 M mannitol and recovered in a round bottom tube (VWR) at 37 °C on a rotary shaker (120 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... myofibril pellets were resuspended in 300 μL of ice-cold wash buffer (Buffer 2: 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 1 mM DTT; all chemicals from VWR), and tubes were centrifuged at 3,000 g for 10 minutes at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted fractions were pooled and further purified on a 1 ml HiTrap FF Q column (VWR 17-5053-01) using an AKTA FPLC system (Cytiva) ...
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bioRxiv - Synthetic Biology 2024Quote: HEK293T cells were seeded 1 day before transfection at 100,000 cells/well in 24-well plates (Multiwell cell culture plates, VWR) for the transient transfections ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 20% glycerol, 1 mM DTT, 50 mM spermidine; all chemicals from VWR), and stored at -80°C for analyses described below.
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bioRxiv - Cell Biology 2024Quote: For indirect immunofluorescence experiments cells were trypsinized and transferred either to Ø 18 mm cover glasses (No. 1; VWR) in 12-well tissue culture plates (TPP ...
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bioRxiv - Microbiology 2024Quote: ... The aqueous phase containing the DNA of each sample was transferred to a fresh conical tube prepared with 1/10th volume of 3M sodium acetate pH 5.2 (3032-16, VWR) and 1 volume of isopropanol (3032-16 ...
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bioRxiv - Neuroscience 2024Quote: ... O-1-) on the BD FACS ARIA III into standard sorting collection buffer (PBS + 10% FBS (VWR 89510-188)) ...
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bioRxiv - Physiology 2024Quote: ... and Alexa Fluor® 488 AffiniPure Don-key Anti-Mouse IgG (H+L) (Cat #102650-156, 1:300, VWR). The analysis of GR translocation was carried out using ImageJ software on 5-6 images per sample acquired from a Nikon Eclipse Ti-U microscope.
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bioRxiv - Neuroscience 2024Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1 hour each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Biophysics 2024Quote: Glass-bottom 384-well plates (Brooks, MatriPlate MGB101-1-2-LG-L) and cover glass (VWR, USA, 48393-251) were passivated with PEG-silane (MW = 5 kD ...
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bioRxiv - Biophysics 2024Quote: ... The collector was changed to a flat aluminum plate with a glass cover (#1 micro cover glass VWR Scientific) placed on the top ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg RNA from each condition was used to generate cDNA with qScript cDNA SuperMix (VWR Cat#101414-104) according to manufacturing protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary PSCs were seeded in 6 well dish at 1 x 104 confluency in Iscove’s modified Dulbecco’s medium (IMDM; Cytiva, SH30228.02) containing 10% FBS (VWR, 97068-085) and 1% Antibiotics-Antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...