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Citations for VWR :
1051 - 1100 of 1121 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The blots were incubated in 5% nonfat dry milk prepared in 1X Tris-buffered saline with Tween 20 (TBS-T, VWR International, #J63682.K2) for 30 min at room temperature to block nonspecific binding sites ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Microbiology 2020Quote: ... Both filtered and unfiltered water samples were vortexed and diluted 1:5.75 into 2% (vol/wt) trace-grade nitric acid (VWR, Radnor, PA) in acid-washed polypropylene tubes and stored overnight at ambient temperature ...
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bioRxiv - Microbiology 2020Quote: ... Pigment-expressing GBS was concentrated in 50 µL of PBS and a 2 µL droplet was aliquoted and sandwiched between two cover slides (48393-230, VWR International). Changes in pigment presence were determined by measuring the change in Raman peak amplitudes associated with conjugated double bonds located at the 1126 cm-1 and 1510 cm-1 before and after light treatment ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Immunology 2020Quote: ... and the filter was washed with sterile 1x phosphate buffer saline (PBS) supplemented with 2% v/v fetal bovine serum (FBS, VWR, USA). Cell density was determined through trypan blue exclusion ...
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bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...
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bioRxiv - Biochemistry 2020Quote: ... The 168 h incubation samples were sonicated for 2 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). Circular dichroism (CD ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in PBS with 2% FBS and samples were stored in PBS containing 1% formaldehyde (VWR Life Science AMRESCO). For intracellular staining ...
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bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- + bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- +bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media with and without thiamine in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 mL LB or BHI medium was inoculated with 20 µL preculture and grown in a 24-well plate (VWR, USA). Furthermore ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Molecular Biology 2024Quote: ... in 600 µL medium and co-transfected 2 hours after seeding with 300 ng per plasmid (600 ng total) by using the jetOPTIMUS transfection kit (VWR; 101000051) according to the instruction manual ...
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bioRxiv - Physiology 2021Quote: ... and this portion was placed on top of a stack of 4-6 glass microscopy slides (depending on size of animal) (VWR, Radnor PA-Cat. No. 48300-026). Tissue was secured onto glass slide with a biorthogonal silicon elastomer (Kwik-Sil ...
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bioRxiv - Neuroscience 2022Quote: ... Microscope slides with serially cut nerves were rinsed in 1x PBS for 5 min and air dried by incubation at 60°C for 45 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with 90% trypan blue for 5 min and trypan blue exclusion was measured using SD100 counting chambers (VWR; Cat. No. MSPP-CHT4SD100) and a cell counter (Nexcelom Cellometer Auto T4) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed twice (5 min each) in RNAase and DNAase free PBS before mounting onto Superfrost Plus coated slides (VWR, catalog no: 48311-703) in RNase- and DNase-free PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were first incubated for 1 h in blocking solution (0.01 M PBS with 0.3% Triton X-100, Sigma, T9284; 5% normal donkey serum, NDS, VWR, 80054-446 or Calbiochem, 566460-5ML) at room temperature ...
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bioRxiv - Genetics 2024Quote: ... 5% glycerol) supplemented with protease inhibitors (Leupeptin, Sigma-Aldrich, 11034626001; Aprotinin, Santa Cruz Biotechnology, sc3595; Pepstatin A, VWR, 97064-248; PMSF, Sigma-Aldrich, 7110), phosphatase inhibitors (Sodium Orthovanadate ...
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bioRxiv - Microbiology 2021Quote: ... and cleared refolded StaR was supplemented with additional 2 mM DTT and concentrated using a centrifugal filter with a MWCO of 3 kDa (VWR #516-0227P) and stored at −80 °C ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
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bioRxiv - Bioengineering 2022Quote: ... a 3 mg/mL ORO stock was prepared by dissolving ORO powder in 100% 2-proponal (VWR #BDH2032-1GLP, Radnor, PA, USA). That stock solution was then diluted to a 1.8 mg/mL working solution in UltraPure™ Distilled Water (ThermoFisher #10977015) ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with secondary antibodies and DAPI for 2 hr at room temperature prior to mounting in AquaPoly/Mount mounting medium (VWR, 87001-902).
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... was defined by completely demineralizing a 2% gelatin solution by adding an abundance of mixed bed ion exchange resin Amberlite IRN-150 (VWR, 40992.A9) to the gelatin ...
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bioRxiv - Microbiology 2024Quote: ... Six clams taken from the acclimation aquarium were crushed together for 2 min at maximum speed using the Stomacher® 3500 instrument (VWR, Italy). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... An incision was made above the quadriceps and either a 2 mm (for sub-critical VML analysis) or a 3 mm (for critical VML analysis) biopsy punch (VWR, 21909-136) was used to make a full-thickness muscle defect28 ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was passed through a 0.22 µm filter before 0.5 ml was combined with 2 ml of Patton-Reeders solution (0.105 mM calconcarboxylic acid, VWR, in 0.73 M NaOH) and vortexed briefly to mix ...