Labshake search
Citations for VWR :
1001 - 1050 of 1054 citations for 8H Purin 8 one 1 7 dihydro 6 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... was achieved using an adapted protocol from Ly et al.22 FFPE TMAs were first washed twice for 1 minute in a xylene bath (mixture of isomers; ≥98.5%, AnalaR NORMAPUR®, VWR, Leicestershire, UK). Residual xylene was removed ...
-
bioRxiv - Neuroscience 2019Quote: ... the aggregates were washed 3× in a 15-ml tube by sedimentation in 3:1 medium and then maintained in suspension on sterile petri dishes (VWR, #391‒2016) in 10 – 15 ml 3:1 medium with media changes every other day ...
-
bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
-
bioRxiv - Microbiology 2019Quote: ... 2 µL of 1:10 diluted purified anti-SpB antibody1 and 2µL of Cy3 conjugated Goat Anti Rabbit IgG Polyclonal Antibody (VWR R611-104-122) were added to 40 µL of 0.4 O.D ...
-
bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
-
bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
-
bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reaction mixture was incubated at room temperature for 60 mins before quenched by adding 12 µL 1% formic acid (VWR, Radnor, PA).
-
bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
-
bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
-
bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
-
bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
-
bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resin was equilibrated with wash buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) and bound proteins were eluted in wash buffer supplemented with 20 mM glutathione ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM dithiotreitol (DTT; [VWR Chemicals])) supplemented with protease inhibitor [cOmplete ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
-
bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
-
bioRxiv - Biophysics 2019Quote: ... cells were grown in 6-well plates (STARLAB; Milton Keynes, UK) or 30 mm culture dishes (STARLAB) on untreated 30 mm borosilicate coverslips (Thickness # 1, VWR International; Radnor, PA) or on poly-D-lysine coated FluoroDishes (FD35-PDL-100 ...
-
bioRxiv - Biophysics 2020Quote: ... The sheath and sample fluid syringes (BD Luer-Lok™ 5-ml syringe #613-2043P and BD Luer-Lok™ 1-ml syringe #613-4971; VWR, Germany) were connected to the fabricated chip via FEP Tubing (1/16” OD ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: 24 h starved polyps were incubated in 1 mM linalool for 10 min in a 60 mm tissue culture dish (VWR International, Radnor, PA). Each animal was pinched using a pair of Dumont No ...
-
bioRxiv - Biophysics 2020Quote: A perfusion chamber was prepared by sandwiching a cover glass (BDH, 22 × 32 mm #1) on top of acid and plasma cleaned cover glass (VWR 24 × 50mm #1.5) containing two lines of Corning High Vacuum Grease ...
-
bioRxiv - Bioengineering 2019Quote: ... and β3TUB is a tubulin thought to be specifically involved during differentiation of neuronal cell types [69].) Antibodies were diluted in antibody dilution buffer (PBS containing 1% bovine serum albumin (VWR, Radnor, PA, USA) and 0.3% Triton X-100 (Sigma-Aldrich)) ...
-
bioRxiv - Neuroscience 2021Quote: ... and 4% TCA (100μl) were added to these tubes and vortexed for 1 minute on a multi-tube vortex mixer (VWR International LLC, Radnor, PA).
-
bioRxiv - Neuroscience 2022Quote: ... mEPSCs were recorded at a holding potential of −70 mV in the presence of 50 μM PTX and 1 μM tetrodotoxin (TTX; VWR, Catalog#89160– 628). Electrophysiological data were analyzed using Clampfit 10.5 (Molecular Devices ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were mixed with the scintillation liquid and counted in a scintillation counter (Packard TriCarb Model, 1 min/vial) using Solvent-Saver™ scintillation vials (VWR International LLC.).
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (HeLa) or 15% (Tig-1) fetal bovine serum (HyClone, GE Healthcare Life Sciences, Marlborough, MA; Avantor Seradigm, VWR, Radnor, PA), 100 units/mL penicillin and 100 µg/mL streptomycin (Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
-
bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
-
bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
-
bioRxiv - Immunology 2023Quote: ... The wells were emptied and blocked with 300 µL of PBS containing 1% BSA (Bovine Serum Albumin Fraction V, VWR Cat# 97061-416) for 1 h at 25°C ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
-
bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
-
bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
-
bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
-
Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...
-
bioRxiv - Neuroscience 2020Quote: ... was mixed with deuterium oxide (450 µl) containing sodium formate (1 µmol/L) and then passed through a 10 KDa cutoff centrifugal filter (VWR, Radnor, PA, United States). 1H-NMR spectra were acquired using a 600 MHz spectrometer (Bruker AVANCE II ...
-
bioRxiv - Synthetic Biology 2020Quote: ... For that purpose specially designed single-use imaging chambers were made using 18 mm × 18 mm cover glasses #1 (VWR, Cat No. 631-1331) and silicon seals cut out of ultra thin silicone film with 0.3 mm thickness (Silex ...
-
bioRxiv - Microbiology 2019Quote: ... Colonies of each bacterial test isolate and a positive and negative control isolate were harvested with a 1 μl loop (VWR International, catalog # 12000-806) from blood agar (BD Diagnostics Systems ...
-
bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
-
bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...
-
bioRxiv - Microbiology 2023Quote: ... The suspension was allowed to cool at room temperature (RT) for 10 min before adding 1 µl RNAse at 10 mg/ml (VWR cat no. 0675-250MG) and incubation at 37 for 60 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Elma Schmidbauer GmbH), mixed (10 min, 2035 rpm, HeidolphTM MultiReax) and centrifuged (1 min, 12300 RCF, Micro-Star 12, VWR®, Radnor, PA, USA). The total protein concentration in each sample was determined using the BCA assay in 10-20 μl sample aliquots ...
-
bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...