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Citations for VWR :
951 - 997 of 997 citations for 8 FLUORO 4H THIENO 3 2 C CHROMENE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... the aggregates were washed 3× in a 15-ml tube by sedimentation in 3:1 medium and then maintained in suspension on sterile petri dishes (VWR, #391‒2016) in 10 – 15 ml 3:1 medium with media changes every other day ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then incubated for 10 min at 65 °C and then for 25 min at room temperature with rotation (Tube Rotator, VWR, Radnor, Pennsylvania). Beads were washed with Millipore water ...
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bioRxiv - Cancer Biology 2019Quote: ... in RPMI with 5% FBS and 1% penicillin/streptomycin for 20 minutes in a humidified incubator at 37°C Tissues were washed with PBS and further dissociated in 0.25% Trypsin (VWR, Cat. # VWRL0154-0100) for 30 minutes in the incubator ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL, VWR, Fontenay-sous-Bois, France). Absorbencies were read at 450 nm.
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bioRxiv - Plant Biology 2021Quote: ... Then 200 µL PGM was gently added to the petridish and it was placed in a humid box and incubated at 23°C (incubator, VWR, 89511-416) for 4 or 7 h ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
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bioRxiv - Immunology 2020Quote: ... All tissue was digested at 37°C for 30 min with intermittent vigorous shaking before being passed through a 70 μm strainer (VWR, 734-0003) and neutralized with 8% FCS-DMEM buffer ...
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bioRxiv - Microbiology 2022Quote: Y-strain epimastigotes were cultured in LIT/LDNT medium (37) supplemented with 15% 76°C heat-inactivated fetal bovine serum (FBS) (VWR, USDA certified). To obtain amastigotes ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Physiology 2023Quote: ... we kept the chamber in a 25 −28 °C water bath and then heated the chamber at a rate of 1 °C · min-1 in a water bath (VWR, model: WBE05) containing 2 L of water ...
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bioRxiv - Microbiology 2022Quote: ... albicans isolates were initially cultured by inoculating a single colony in sterile YPD broth at 30°C in an orbital shaker (VWR, model 3500I) at a shaking speed of 200 rpm at a tilted angle of 45° ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Microbiology 2022Quote: ... Blood was collected by cardiac puncture and then was maintained at 37°C in incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412). Infected red blood cells were enriched by an Accudenz discontinuous gradient as previously described [30] ...
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bioRxiv - Microbiology 2020Quote: ... The liquid and solid fractions of a sample were separated by filtration through a combination of three layers of sterile cheesecloth that had been wrapped separately and baked at 175°C and then transported in sealed 15 ml conical Falcon tubes (catalog number: 62406-200, VWR International, Radnor, PA) on dry ice to the laboratory and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were sliced into 1mm thick coronal slices at -20°C using a metal brain matrix and .22mm razor blades (Ted Pella, 15045; VWR, 55411-050) and were then placed on dry ice and covered to prevent condensation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with 250 nM TG or 10 µM monensin in fresh medium for 0.5 h at 37°C before 40 µM D-biotin (VWR Life Science, Radnor, PA) was added ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was centrifuged (20 min at 4000 rpm) and the supernatant was evaporated (25°C at 210 rpm) on a rotary evaporator (IKA RV10 digital, VWR, Radnor, PA, USA). The dried crude extract was resuspended in 1 ml of acetonitrile for further analyses ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... Cultures were routinely cultivated in growth chambers (LED-41L2, Percival Scientific, Inc.) at 25°C with continuous shaking on an orbital shaker (VWR OS-500 Shaker) at 120 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed off the filter with an ice-cold freshly prepared mixture of methanol/ethanol/chloroform (1:3:1) (ethanol LiChroSolv©, Merck, Darmstadt, Germany; chloroform HiPerSolv©, VWR, Dresden, Germany). Extracts were treated in an ultrasonic bath for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... The plates were incubated for four hours at 37 °C under shaking conditions (175 rpm) using a benchtop incubator (VWR® incubator orbital shaker). The 96-well plate was kept inside an airtight plastic container with several wet paper towels to maintain high humidity attached to the shaker ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
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bioRxiv - Neuroscience 2022Quote: ... Microscope slides with serially cut nerves were rinsed in 1x PBS for 5 min and air dried by incubation at 60°C for 45 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −20 °C in an anti-freeze solution (30 % glycerol [Merck Millipore, Germany], 30 % ethylene glycol [VWR International, USA] and 10 % TBS) until further processing ...
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bioRxiv - Neuroscience 2024Quote: ... Microscope slides with serially sectioned nerves were rinsed in 1× PBS for 5 min (repeated twice) and air-dried by incubation at 70°C for 10 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Microbiology 2021Quote: ... and a dye photosensitizer in N,N-dimethylformamide/acetone (7/3, v/v) was electrospun onto one layer of polypropylene (PP) fabrics (VWR® Basic Protection Face Mask). During the electrospinning of 10-20 wt% of PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... for cryoprotection and allowed to sink at 4°C prior to being cryosectioned at 20 μm and placed on charged slides (VWR Superfrost® Plus, 48311-703).
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35150™) was cultured overnight in 3 mL of brain heart infusion (BHI) growth medium (VWR International, LLC, 95021-488) using a shaker-incubator at 37 °C ...