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Citations for VWR :
951 - 1000 of 1089 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... pre-cultures for reporter-based promoter activity assays were performed at 30°C and 900 rpm in 1 ml medium in 2.2 ml square-shaped V-bottom 96-deep well plates (VWR, Radnor, US-PA) covered with a sterile breathable rayon film (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was equilibrated with wash buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) and bound proteins were eluted in wash buffer supplemented with 20 mM glutathione ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM dithiotreitol (DTT; [VWR Chemicals])) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Biochemistry 2024Quote: ... The enzymatic reaction incubated for 60 min at room temperature followed by quenching with 80 μL 1% formic acid (VWR, Radnor, PA) containing 0.03 µg/µL S-adenosylhomocysteine-d4 (d4SAH [Cambridge Bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... was conducted in 1x Towbin buffer with 0.1 % SDS for 30 minutes (25 mM Tris-base, 192 mM Glycine, 1 % SDS) using a PerfectBlue Semi-Dry Electroblotter Sedec M (VWR; 700-1220). Transfer was performed at a constant current of 10 mA for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and the wing was imaged at selected ages through a cover glass of thickness 1.5 (VWR Micro Cover Glass, selected 1 ounce, 18×18 mm, No. 1.5, VWR Cat No 48366-205).
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bioRxiv - Evolutionary Biology 2021Quote: ... which we then incubated at 25°C for 4 days prior to staining with iodine (VWR iodine crystals) vapor (Forsburg and Rhind 2006).
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bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was kept overnight in a solution of PBS 0.01 mM/formaldehyde 4% (v/v; CAT#20909.330, VWR) and then cut in 50 μm slices with a vibratome (VT1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were then perfused with 30 mL of cold PBS and 4% paraformaldehyde (#100503-916, VWR, Radnor, PA) and were immediately decapitated to collect the brains ...
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bioRxiv - Developmental Biology 2021Quote: ... Fetuses and brains were then stored long-term at 4°C in 0.002% Sodium Azide (VWR - Bie&Berntsen) in PBS.
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bioRxiv - Physiology 2023Quote: ... a reversed-Phase C18 LiChroCART 250-4 column (LiChrospher 100 RP-18, 5 μm, VWR, Radnor, PA, USA) was used ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2022Quote: Cells were mounted on ∼1.4% w/v agar pads (in sterile ultrapure water) and a 0.13- to 0.17-mm glass coverslip (VWR) was placed on top ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were fixed with 4% PFA for 15 min and incubated with 40 mM Alizarin Red S (VWR, US) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... RLMVECs assayed for Mx1/2/3 expression during SEOV infection were fixed using 4% paraformaldehyde (VWR, 97064-606) solution in sterile PBS for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... reference slides were generated using 4-micron thick sections mounted on SuperFrost Plus slides (VWR International, Lutterworth, UK) and reviewed by the neuropathologist who performed the autopsy ...
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bioRxiv - Developmental Biology 2024Quote: Fish were anesthetized using tricaine and initially fixed by immersion in a solution of 4% paraformaldehyde (VWR:15713) and 0.1M sodium-cacodylate (VWR:11653) ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Biophysics 2020Quote: ... The sheath and sample fluid syringes (BD Luer-Lok™ 5-ml syringe #613-2043P and BD Luer-Lok™ 1-ml syringe #613-4971; VWR, Germany) were connected to the fabricated chip via FEP Tubing (1/16” OD ...
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bioRxiv - Biophysics 2020Quote: A perfusion chamber was prepared by sandwiching a cover glass (BDH, 22 × 32 mm #1) on top of acid and plasma cleaned cover glass (VWR 24 × 50mm #1.5) containing two lines of Corning High Vacuum Grease ...
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bioRxiv - Neuroscience 2021Quote: ... and 4% TCA (100μl) were added to these tubes and vortexed for 1 minute on a multi-tube vortex mixer (VWR International LLC, Radnor, PA).
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bioRxiv - Neuroscience 2022Quote: ... mEPSCs were recorded at a holding potential of −70 mV in the presence of 50 μM PTX and 1 μM tetrodotoxin (TTX; VWR, Catalog#89160– 628). Electrophysiological data were analyzed using Clampfit 10.5 (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were mixed with the scintillation liquid and counted in a scintillation counter (Packard TriCarb Model, 1 min/vial) using Solvent-Saver™ scintillation vials (VWR International LLC.).
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (HeLa) or 15% (Tig-1) fetal bovine serum (HyClone, GE Healthcare Life Sciences, Marlborough, MA; Avantor Seradigm, VWR, Radnor, PA), 100 units/mL penicillin and 100 µg/mL streptomycin (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
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bioRxiv - Immunology 2023Quote: ... The wells were emptied and blocked with 300 µL of PBS containing 1% BSA (Bovine Serum Albumin Fraction V, VWR Cat# 97061-416) for 1 h at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Neuroscience 2024Quote: ... and stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then resuspended again in FACS buffer depending on staining condition either with or without DAPI at 1:5000 (VWR, cat# 89139-050) and transferred to FACS tubes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Immunology 2020Quote: These assays were done using methods similar to those described (52) and Immulon 4 microtiter plates (VWR, Radnor, PA) coated with 100ng per well of S1 ...
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bioRxiv - Immunology 2023Quote: ... then placed overnight at 4°C in a 25% sucrose solution containing 50% optimal cutting temperature compound (OCT, VWR Clear Frozen Section Compound ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were collected onto copper mesh grids and contrasted for 2min with 4% uranyl acetate solution in methanol (VWR), followed by 2min in lead citrate (Reynold’s solution) ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37 °C for 25 min while stirring at 900 RPM (speed 4/6) on a hotplate stirrer (VWR). The digested suspension was strained through a 70-um strainer ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Microbiology 2022Quote: ... Brains and lungs were collected at 4-days post inoculation and homogenized in PBS with a benchtop homogenizer (VWR). Homogenates were diluted in a 10-fold dilution series and each dilution was plated on YPD ...
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bioRxiv - Neuroscience 2024Quote: ... iN cells were fixed after differentiation at d21 for 20 minutes at room temperature (RT) with 4 % Paraformaldehyde (PFA) (47377.9L, VWR) and 4 % sucrose (S9378 ...
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bioRxiv - Biochemistry 2024Quote: ... sectioning into 4 µm sections and mounting on superfrost plus slides (Cat# 631-9483, VWR, Avantor, Radnor Township, PA). Researchers had access to medical records.
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...