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Citations for VWR :
51 - 100 of 158 citations for Recombinant Mouse Kit His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Total RNA Kit I (VWR, #CA101319) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Mouse brains were collected through flash freezing in OCT Tissue Tek medium (VWR #25608-930) in dry ice ...
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bioRxiv - Molecular Biology 2023Quote: Mouse and chicken brains were dissected and immediately immersed in OCT embedding medium (361603E, VWR) for a washing step ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated donkey antirabbit (NA934V) and anti-mouse (NA931-1M (both from VWR International Ltd.) secondary antibodies were used at 1:3000-5000 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse brains were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 24 h ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in an optimized mouse prostate primary cell medium composed of DMEM/F12 (VWR, 45000-344), 10% FBS ...
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bioRxiv - Genomics 2019Quote: ... Western blots were performed using a mixture of mouse anti-actin antibodies (VWR catalog number 89500-294) and rabbit anti-TAP antibodies (ThermoFisher catalog number CAB1001 ...
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bioRxiv - Bioengineering 2020Quote: The mouse hearts were first decellularized in 0.25% and 0.5% (wt/vol) sodium dodecyl sulfate (SDS, VWR) solution at RT for 24 h or until the tissues turn transparent white with constant agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mouse brains and flank tumors were homogenized in a pre-chilled glass tissue grinder (VWR) with RIPA lysis buffer containing protease and phosphatase inhibitors (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies were purchased from VWR. The GFER rabbit polyclonal antibody (#HPA041227 ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated using the peqGOLD Total RNA Kit (VWR) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated using TriFast RNA extraction kit (30-2030, VWR) or RNA-Solv reagent (Omega Bio-tek) ...
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... The COD was determined using the Spectroquant COD kit (VWR, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA Kit I (Omega BIO-TEK; VWR catalog no. 101319) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoblots were then washed with TBS containing 0.05% Tween 20 (TBST) and incubated for 1 hour at ambient temperature with either anti-mouse horseradish peroxidase conjugated (NA931, VWR; 1:5000); or anti-rabbit HRP conjugated (NA934 ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Neuroscience 2021Quote: ... extracted with the RNA extract kit (1x106 cells-10mg; VWR International PBI) and retrotranscribed with iScript Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted using the Zymo Quick-RNA MiniPrep kit (VWR, R1055) according to the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted using the Zymo Quick-RNA miniprep kit (VWR; R1055) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted using the Direct-zol RNA miniprep kit (VWR 76211-340). cDNA synthesis was performed using hexamer primers and SuperScript IV Reverse Transcriptase (Thermo Fisher 18090010) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Molecular Biology 2022Quote: ... The total soma RNA was extracted with the RNA extraction kit (VWR International PBI) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription was performed using the qScrpit cDNA synthesis kit (VWR, catalog # 95048-100) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA using the Life Science Yeast Genomic DNA Purification Kit (VWR). We sent the samples for library preparation and sequencing at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was isolated from the cells with Zymo Quick-RNA Miniprep kit (VWR, #76299-632) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).