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Citations for VWR :
51 - 100 of 153 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Neuroscience 2020Quote: Snap frozen human DRGs were sectioned onto superfrost plus glass slides (VWR International, Leuven, Belgium) at 10μm thickness ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Genomics 2023Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR 25608-930) and sectioned on a Leica cryostat at -17C at 10 μm onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
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bioRxiv - Immunology 2023Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a 0.45 µm nitrocellulose membrane (VWR), by electroblot ...
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bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin embedded human trephine and tonsil tissues were sliced on to Superfrost Plus® slides (VWR) and incubated at 60°C for 45min ...
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bioRxiv - Biophysics 2021Quote: Peripheral blood monocytes were isolated from human blood using 15 Ml Ficoll-Paque (VWR, Darmstadt, Germany) centrifugation gradient ...
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bioRxiv - Microbiology 2019Quote: The human monocyte cell line U937 (ATCC CRL-1593.2) was cultured in RPMI 1640 media (VWR) supplemented with 10% FBS at 37C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Human neuroblastoma SH-SY5Y cells (ATCC, CRL-2266) were cultured in DMEM medium (VWR, 0101–0500) supplemented with 10% FBS (Gemini Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and human keratinocyte growth supplement (HKGS) 100X (S-001–5) from Cascade Biologist were procured from VWR Corporation (Missouri ...
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bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
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bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...