Labshake search
Citations for VWR :
51 - 100 of 132 citations for Recombinant Human Long Arg3 Insulin like Growth Factor I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA Kit I (Omega BIO-TEK; VWR catalog no. 101319) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Growth curves were acquired by following OD with a Biowave cell density meter CO8000 (VWR International). Growth rates were inferred by fitting growth-curves to OD as a function of time with a four-parameter logistic function was used70.
-
bioRxiv - Microbiology 2021Quote: ... Growth curves were acquired by following OD with a Biowave cell density meter CO8000 (VWR International). Supernatant samples were collected and processed as explained above ...
-
bioRxiv - Neuroscience 2024Quote: ... Intraperitoneal injection of 20ul of the Amp-101 into Dok-7KI/KI mice or saline into WT littermates was carried out on P4 using 0.3ml 30g insulin needles (VWR International Ltd). Mice were weighed every day from P3 until P30 after which they were weighed once per week.
-
bioRxiv - Bioengineering 2021Quote: ... Vascular compartments were coated with a rat tail collagen type I (VWR) solution of 0.1 mg/ml in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... We confirmed the amplification using 2% Agarose I™ (VWR, Philadelphia, PA) and gel electrophoresis ...
-
bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
-
bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
-
bioRxiv - Plant Biology 2022Quote: ... directly connected to a computer and monitored using the i-Weight software (VWR). Plant recovery was done by placing potted plants directly in water for 72h ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumor growth was monitored weekly and tumor volume was measured along 3 axes with calipers (VWR 62379-531). Tumors and lungs were harvested at time of tumor burden (total tumor volume of 2cm3 ...
-
bioRxiv - Biochemistry 2020Quote: ... The selected colony was then inoculated into a midiprep-scale volume of liquid Luria-Bertani growth medium (VWR) and plasmid DNA isolated using a PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Scientific) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
-
bioRxiv - Microbiology 2020Quote: ... Growth media were loaded into plastic 10 mL syringes (Codan) or glass vials (VWR, cat. no. 548-0154) and warmed to 37 °C at least 3 h prior to the start of each experiment.
-
bioRxiv - Genetics 2020Quote: ... after which the media was changed to complete growth medium and the cells expanded in T182.5 flasks (VWR) in monolayer and until confluent (between 10-14 days) ...
-
bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were rocked every 15 minutes for 1 hour before a semi-solid overly containing 0.5% Methylcellulose in viral growth media (VWR) was applied ...
-
bioRxiv - Systems Biology 2022Quote: ... we isolated the growth media from liquid cultures by flowing liquid cultures through a 0.2 µm pore filter (VWR). We ensured that no cells remained in the filtered media by flowing an aliquot through our flow cytometer ...
-
bioRxiv - Microbiology 2022Quote: ... Optical density at 450 nm (OD450) was measured to estimate the bacterial growth curve using (VWR V-3000PC spectrophotometer). The final OD450 values were determined by subtracting the 2.51-multiplied sample well value with the blank well value.
-
bioRxiv - Biophysics 2023Quote: ... this inactivation solution was replaced with growth medium containing 10% horse serum (S900-500, VWR International GmbH, Darmstadt, Germany), 1.8 mM glutamine ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 50 ng/ml recombinant murine macrophage-colony stimulating factor (rmM-CSF, Genentech)] and plated in 15-cm non-TC treated dishes for 5 days (Petri dish, VWR, 25384-326). Fresh BMDM media was added on day 3 without removal of original media ...
-
bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
-
bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
-
bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
-
bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
-
bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
-
bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were seeded on 24-well plate (0.5 x 105 cell/well) in growth medium (DMEM medium; Thermo Fisher Scientific, 10569010, fetal bovine serum; VWR, 97068-085 ...
-
bioRxiv - Neuroscience 2022Quote: ... The tissue was then triturated in 10ml of warm growth medium and passed through a 70μm cell strainer (VWR #10199-657) to remove large particulates ...
-
bioRxiv - Cancer Biology 2022Quote: ... medium was replaced by 60 μL of 5 mg/mL Collagen Type I (VWR, 734-1085) in 1M NaOH and 1X DMEM/F12 ...
-
bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
-
bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
-
bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... All cultures were grown in 10mL of the growth medium in 50mL flasks (FisherSci #FS2650050) capped with 50mL plastic beakers (VWR #414004-145) at room temperature (21°C ...
-
bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
-
bioRxiv - Neuroscience 2020Quote: ... and DNase I (20μg/ml) at 37°C for 75 minutes in an incubating mini shaker (VWR). The digested brain material was pelleted by centrifugation and resuspended in 20% bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
-
bioRxiv - Cancer Biology 2021Quote: 1×105 A549 or H460 cells in 200 µl of serum-free growth medium were seeded in the insert of a transwell plate with 8 μm pores (VWR International, Radnor, PA). These cells were coincubated with AvFc or CTX at 30 nM for 2 hours at 37 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... Cultures were routinely cultivated in growth chambers (LED-41L2, Percival Scientific, Inc.) at 25°C with continuous shaking on an orbital shaker (VWR OS-500 Shaker) at 120 rpm ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants collected from aliquots in the CFU growth arrest assay described above were filtered with 0.45um Supor® Membrane filters (VWR, Cat# 28143-352). 1:10 serial dilutions were carried out and 2ul of each dilution was plated onto the top agar containing RN4220 ...
-
bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
-
bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
-
bioRxiv - Cell Biology 2019Quote: ... 50 µL of the collagen-I mixture was pipetted into the bottom of the wells of an 8-chamber slides (VWR) to coat the surface and allowed to gel for 20 minutes at 37°C ...
-
bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...