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Citations for VWR :
51 - 100 of 211 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Reactions were incubated in a nuclease-free PCR tube (VWR) in a Thermal Cycler (C1000 Touch ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted with direct PCR Lysis Reagent Cell (VWR). RNA isolation was performed using the AMBION PureLink RNA Mini kit (Themo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... were added to the wells of 96-well PCR plates (VWR) in a final volume of 25 μl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... attached to an autoclaved 1.2 ml 96-well PCR plate (VWR). We centrifuged the samples in the filter plates together with the collection plates for 15 min at 2,500 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... reconciled into a clear 96-well PCR plate (VWR #76402-848), and sealed with adhesive plate tape ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted using Direct PCR Lysis Reagent Cell (VWR). RNA was extracted using the AMBION PureLink RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... and 10 µL PCR grade nuclease-free water (VWR, Radnor, PA) were combined ...
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bioRxiv - Genetics 2021Quote: ... Resulting PCR products were loaded on a 2% agarose (VWR, 97062-250) gel ...
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bioRxiv - Cell Biology 2020Quote: ... The Brilliant II SYBR Green QRT-PCR 1-Step Master Mix (VWR) was used to convert RNA to cDNA and amplify specific RNA regions on the CFX96 Touch real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were amplified with a peqSTAR thermocycler (VWR, Radnor, PA, USA) and the following conditions ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (VWR). Primers were designed for gene specificity and to cross exon-exon junctions using Primer-BLAST (www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Zoology 2022Quote: ... purified using ExoCleanUp FAST PCR clean-up reagent (VWR Life Sciences USA), sequenced at the forward direction in outsourcing at StabVida (Portugal ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were visualized under UV light by Photo Imager system (VWR) and the intensity was quantified by ImageJ software (NIH) ...
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bioRxiv - Microbiology 2022Quote: ... The sample mixture was then transferred into an optically-clear PCR tube (VWR). SYPRO orange fluorescence data in relative fluorescence unit (RFU ...
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bioRxiv - Biochemistry 2022Quote: ... The sample mixture was then transferred into an optically clear PCR tube (VWR). SYPRO orange fluorescence data in relative fluorescence unit (RFU ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were cloned into the pTS018 entry vector via Gibson assembly (VWR). The gRNA scaffold was amplified from pSL001 with primers P3 and P4 ...
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bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries ...
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bioRxiv - Developmental Biology 2023Quote: ... Reactions were carried out in 0.2 ml clear PCR tubes (VWR, Philadelphia, PA), and the cycling conditions were ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were analysed on a 1-2 % agarose gel (VWR, #732-2789P) prepared in 1X TAE buffer (VWR ...
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bioRxiv - Synthetic Biology 2023Quote: ... All reactions were incubated in a 96 PCR well plate (VWR #47744-116). Surrounding wells were filled with 1x phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were separated by agarose gel electrophoresis using 1.5% agarose (VWR, 0710) in 1X TBE buffer at 150V.
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with Red Taq DNA Polymerase Master Mix (VWR Life Science) according to the manufacturer’s instructions and purified with magnetic beads (CleanNGS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Karyotyping PCRs were conducted using RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) with the following published primers ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... All PCR reactions were conducted using KAPA Hotstart HiFi ReadMix (VWR, Cat#103568-584) with annealing temperature at 62°C (15 sec ...
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bioRxiv - Microbiology 2021Quote: ... Each 20 μL PCR reaction was prepared with 9 μL 5Prime HotMaster Mix (VWR International), 1 μL forward primer (10 μM) ...
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bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were loaded on a 1% agarose gel and stained with EZ-Vision® (VWR). Images were taken using the GelDoc system (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2024Quote: ... a two-fold serial dilution with 1X reaction buffer was conducted in clear PCR tubes (VWR). The methyltransferase reaction was initiated by adding 10 ml of 2x substrate solution (80 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Biophysics 2024Quote: ... and solutions of eluted DNA-encoded compounds were transferred to a 96-well PCR plate (VWR).
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Systems Biology 2024Quote: ... were curated from E.coli DH5a using a plasmid extraction kit (peqGOLD Plasmid MiniPrep Kit II; VWR, Leuven, Belgium). PA01 cells were made competent for transformation by treatment with a sucrose 300 mM solution PA01 ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s recommendations and was added as a template into a one-step multiplex qRT-PCR assay using Quanta qScript XLT ToughMix with ROX dye (VWR). For that ...
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bioRxiv - Synthetic Biology 2020Quote: ... reactions were incubated in PCR tubes prior to irradiation using a UV-transilluminator (VWR, Dual UV Transilluminator, 80 mW/cm2). Following irradiation ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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bioRxiv - Molecular Biology 2024Quote: ... (newly assigned) were used for amplification with a primer concentration of 1 µM and the help of a PCR Mastermix (VWR Life Science ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2024Quote: ... HP Total RNA Isolation Kit (VWR CA101414-852). 1 μg RNA from each condition was used to generate cDNA with qScript cDNA SuperMix (VWR Cat#101414-104 ...