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Citations for VWR :
51 - 100 of 1174 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2 × 105 cells were plated in transwell inserts (VWR, Radnor, PA) coated with 0.3 mg/mL Corning™ Matrigel™ Membrane Matrix (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated donkey antirabbit (NA934V) and anti-mouse (NA931-1M (both from VWR International Ltd.) secondary antibodies were used at 1:3000-5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and was stopped by addition of 2 M sulfuric acid (BCH7500-1, VWR) at 1:1 volume ratio ...
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bioRxiv - Biophysics 2020Quote: ... Before digestion the samples were diluted 1:2 with MS grade water (VWR). Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
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bioRxiv - Cell Biology 2024Quote: ... PCR products were analysed on a 1-2 % agarose gel (VWR, #732-2789P) prepared in 1X TAE buffer (VWR ...
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bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 μg of either wild-type FHL-1 or RGD-null FHL-1 was incubated with 2 μg C3b (VWR International, Lutterworth, UK), and 0.4 μg factor I (FI ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Genomics 2019Quote: ... Western blots were performed using a mixture of mouse anti-actin antibodies (VWR catalog number 89500-294) and rabbit anti-TAP antibodies (ThermoFisher catalog number CAB1001 ...
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bioRxiv - Microbiology 2024Quote: ... smegmatis WT and ΔmftG strains were grown in 320 mL HdB-Tyl medium supplemented with 1 g L−1 ethanol as the sole carbon source in 2 L flasks covered by breathable rayon film (VWR) in triplicates and incubated at 37 °C and 210 rpm with a starting OD600 of 0.1 for WT and 2.0 for ΔmftG for a period of 48 h.
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit HRP conjugated (NA934, VWR; 1:5000) antibodies diluted in blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-tubulin (1:60,000, VWR, 89364-004). Secondary antibodies were Alexa 680 anti-mouse (Life Technologies ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Neuroscience 2022Quote: ... or 2% BSA (2% Bovine Serum Albumin (VWR), 50mM Tris-Cl ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 mL PBS + 1 M NaCl and 50 mL 2 M Urea (VWR, 97063-798) + 50 mM Ammonium Bicarbonate ...
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Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approachbioRxiv - Genetics 2022Quote: ... and these were plugged with 1/2 of a large cotton ball (VWR, 14224-518) dampened with 1-ml of 10% sucrose solution (Fisher Chemical ...
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bioRxiv - Neuroscience 2024Quote: ... We used anti-rabbit HRP (diluted 1:1000, VWR International) as the secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Neuroscience 2019Quote: ... 2% FBS (VWR), doxycycline and ROCK inhibitor ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) in PBT (1:1000; VWR, 89139-054, Radnor, USA) for 10 minutes and washed with PBT twice for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Cell Biology 2023Quote: ... slabs were optically cleared using a 1:2 mixture of benzyl alcohol (Cat. No. 109626; VWR, Sweden) and benzyl benzoate (BABB ...
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bioRxiv - Cell Biology 2023Quote: ... 4-cell stage embryos were mounted on a thin (∼1-2x lab tape thickness) 2% agar pad on a glass slide (VWR VistaVision, 3 inches x 1 inch x 1 mm) using a hand-pulled glass pipette (VWR Pasteur Pipette ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... 1:200 dilution of Donkey anti-guinea pig Alexa Fluor 647 (VWR), and 1:500 dilution of DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 1 hour in 2 ml of 3% BSA in PBS (VWR, cat#: 97063-624). Cells were then washed three times with 2 ml of PBS for 5 minutes each and mounted with Prolong Diamond Antifade Mountant (Molecular Probes ...
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bioRxiv - Genomics 2020Quote: ... 2 mM CaCl2 (VWR International Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... 2% maltose monohydrate (VWR), or 5% glycogen from oyster (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM glutamine (VWR), 100 U/ml of penicillin ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...