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Citations for VWR :
51 - 100 of 1558 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... fixed SW480 cells were blocked with 1% Bovine Serum Albumin (RLBSA50; VWR), 0.1% TWEEN® 20 ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed with 1% sodium dodecyl sulfate (SDS) in PBS (VWR) and sonicated at 50% amplitude for 10 seconds twice ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2 × 105 cells were plated in transwell inserts (VWR, Radnor, PA) coated with 0.3 mg/mL Corning™ Matrigel™ Membrane Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in medium containing 1 μg/ml puromycin (VWR International) and selected for 2 days.
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in 1 mL of cold sorting buffer (L15/10 mM HEPES/1 mg/mL BSA (VWR)/25 μg/mL DNase I (Roche)) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Biochemistry 2022Quote: ... Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044) were incubated 72 h at 27 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were transferred a 1 mm-gap sterile electroporation cuvette (VWR, 732-1135) and electroporated using an ECM 399 Exponential Decay Wave Electroporation System (BTX ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
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bioRxiv - Cell Biology 2020Quote: ... RPE-1 cells were incubated in medium containing 356 µM cycloheximide (VWR Chemicals, 441892A) for 6h.
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed after 48h with 1% sodium dodecyl sulfate (SDS) in PBS (VWR), then proteins were separated on 4-12% Mini-PROTEAN GTX polyacrylamide gel (Bio-rad ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Cells were transferred to 15 ml Precellys sample tubes (VWR BERT KT03961-1-4061), mixed with 1 ml acid-washed glass beads (~400 μm ...
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bioRxiv - Microbiology 2020Quote: ... cell pellets were lysed by the addition of 1 ml peqGOLD TriFast™ (VWR) followed by shaking for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized by treatment with 250 µL PBS + 1 mM CaCl2 (0556, VWR), 1 mM MgCl2 (M8266 ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellets were resuspended in 1X PBS containing 1% formaldehyde (AA33314-K2, VWR) and protease inhibitors ...
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bioRxiv - Biochemistry 2019Quote: ... 1 x 106 cells were pelleted and resuspended in a 1:2.5:1 mixture of H2O: methanol: chloroform (both VWR, Radnor PA, USA) pre-cooled to −20 °C ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with a reaction mixture containing 1 mM CuSO4 (VWR International #470300-880), 5 μM sulfo-Cyanine5 azide (Lumiprobe #B3330 ...
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bioRxiv - Biophysics 2022Quote: ... CV-1 cells were seeded on round coverslips (diameter 18 mm, thickness 0.16 mm, VWR) in 12-well plates in 1 ml of cell culture medium per coverslip ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown on 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) to 30-50% confluency ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 BCBL-1 cells per time point were crosslinked with 2% formaldehyde (VWR M134) in prewarmed 1X phosphate buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... EBY100 cells were thawed by inoculating 5 mL YPD (1% Bacto yeast extract (VWR #90000-726), 2% Bacto peptone (VWR #90000-368) ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were harvested 24 h later using 1 mL TriFast™ (VWR, cat. no. 30-2010) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...