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Citations for VWR :
51 - 99 of 99 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours in cold room ...
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bioRxiv - Genetics 2023Quote: ... DNA was cleaned with phenol-chloroform phase separation with phase lock tubes (VWR, 10847-802). DNA was precipitated with 1 volume isopropanol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
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bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase ...
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bioRxiv - Genetics 2023Quote: ... DNA was cleaned and isolated with phenol-chloroform extraction using phase-lock tubes (VWR, 10847-802). 5 μg was run on a 1.5% agarose gel to ensure fragments of 200-400 bp were generated ...
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bioRxiv - Biochemistry 2024Quote: ... Transient transfections were performed with JetPRIME DNA and siRNA transfection reagent (Polyplus supplied by VWR, 101000046).
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bioRxiv - Evolutionary Biology 2019Quote: ... Phage DNA was extracted using an equal amount of phenol:chloroform:isoamyl alcohol (25:24:1) (VWR Life Science) to lysate ...
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bioRxiv - Genomics 2022Quote: ... 1μL of unnormalized DNA was removed and transferred to a black 96-well plate (VWR, 33501-812) with 99μL of TE (10mM Tris ...
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bioRxiv - Genomics 2023Quote: ... DNA was precipitated with isopropanol and stored in Tris-EDTA buffer at a pH of 8.0 (VWR). DNA purity was assessed via Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA concentration and purity were assessed with a spectrophotometer (NanoDrop® ND 1000, VWR International, Radnor, PA, USA). Library preparation and double-digest restriction site-associated DNA sequencing (ddRAD-seq ...
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bioRxiv - Biochemistry 2021Quote: ... 400 ng DNA was transfected using 0.8 μl Jet Prime reagent in 40 μl Jet Prime buffer (VWR) per well ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Genetics 2023Quote: ... DNA was counterstained with DAPI (0.2ug/mL) and the discs were mounted in Vectashield® (VWR, 101098-042) mounting media and imaged on a Leica Confocal SP8.
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bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Genetics 2020Quote: Plasmid DNA containing the repair template with three tandem attP sites was co-transfected (1:1, Jetprime VWR#89129) with a plasmid containing Rosa26-Cas9/gRNA into mouse ES cells when confluency reached 70% ...
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bioRxiv - Microbiology 2022Quote: ... All PCR products were run in 0.7% agarose gels using 100V for 50 min and visualized under blue light with EZ-Vision Bluelight DNA dye 10,000X (VWR). The thermocycling conditions for all PCRs were ...
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bioRxiv - Microbiology 2023Quote: ... and adding sterile filter double distilled water (liquid medium) or autoclaved molten 2% DNA pure agarose (VWR, Radnor, PA) (agarose plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 50 μL vaccine comprising DNA formulated in PBS was administered using Electroporation (EP) (BTX Agile pulse, VWR). In the remaining three immunizations ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative and qualitative analysis of the isolated DNA was performed using a VWR mySPEC microvolume UV-visible spectrophotometer (VWR Lab Products Private Limited ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the DNA extracted was determined by electrophoresis using a 1.5 % agarose gel and with a spectrophotometer (VWR International) and quantified using the Qubit dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of Okazaki fragments was accomplished by running the denatured genomic DNA through 400 ul Source 15Q (VWR 89128-854), binding at 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... and transfected with 800 ng/ml total DNA (including 50 ng/ml PA-X construct) using jetPRIME transfection reagent (Polyplus transfection, VWR). Cells were harvested 24 hours after transfection for RNA extraction and purification.
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
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bioRxiv - Genomics 2020Quote: ... DNA was then reverse-crosslinked and purified using a standard phenol/chloroform cleanup method following the manufacturer’s protocol (VWR, cat. # VWRV0883). Secondary digestion of purified DNA was performed overnight at 37°C with 50 U of Csp6I (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.4 μl of each primer and 5 μL VWR Red Taq DNA polymerase Master mix (VWR Chemicals, Denmark, Cas nr: 733-2547). The PCR reaction conditions for amplification of DNA were as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500). An appropriate volume of reaction mastermix was prepared with the following composition ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR for UQCRC2 and CACNB2 total transcript levels was performed on previously generated RNA input and RNA IP cDNA using KOD Hot Start DNA Polymerase (VWR CA80511-386). For UQCRC2 ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...