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Citations for VWR :
901 - 950 of 1239 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, 437144N) at 26 °C for 6 h ...
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bioRxiv - Microbiology 2020Quote: ... or after 10-1 dilution in molecular biology grade ddH20 (VWR Chemicals, Darmstadt, Germany) for specific amplification and high throughput sequencing of the fungal rDNA internal transcribed spacer 1 (ITS1) ...
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bioRxiv - Microbiology 2019Quote: ... 1 U of VWR Taq DNA Polymerase (VWR®, WR International bvba/sprl, Belbium), 1 X key Buffer supplemented with MgCl2 (1.5 mM) ...
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bioRxiv - Bioengineering 2020Quote: ... Both methods of surface modification were applied to 1” x 3” glass slides (VWR). A small volume (1.75 µL ...
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bioRxiv - Neuroscience 2020Quote: ... cooled down with −1 °C/min in a cryo container (Mr. Frosty™, VWR) and stored at −80 °C or in liquid nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... an equal volume of 25:24:1 phenol-chloroform-isoamyl alcohol (VWR, Radnor, PA) was added and the tubes were vortexed for 30 s followed by centrifugation at 10,000g for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a washing step with 3 mL 1% acetonitrile (ACN; VWR, 83640-290)/0.1% TFA and elution with 500 µL 40% ACN and 500 µL 60% ACN ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked at room temperature for 1 h using Rockland blocking buffer (VWR). Primary PDI antibody (Abcam ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed after 48h with 1% sodium dodecyl sulfate (SDS) in PBS (VWR), then proteins were separated on 4-12% Mini-PROTEAN GTX polyacrylamide gel (Bio-rad ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Colon tissues were homogenized for 1 minute using a disperser (T10 ULTRA-TURRAX; VWR) on ice ...
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bioRxiv - Microbiology 2019Quote: ... A total of 1 x 108 CFU was added to fresh sheep blood (VWR) in V-bottom 96-well plates (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1 hour, washed with Wash media (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-cavin3 (Cat no. ABNOH00112464-MO4, 1:200 PLA, VWR International Pty, Australia), rabbit anti-BRCA1 C-20 (SC-642 ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 ml of pre-chilled (−20°C) methanol/acetic acid (3:1, VWR chemicals) was added dropwise and flicking to homogenize ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Cells were transferred to 15 ml Precellys sample tubes (VWR BERT KT03961-1-4061), mixed with 1 ml acid-washed glass beads (~400 μm ...
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bioRxiv - Microbiology 2020Quote: ... cell pellets were lysed by the addition of 1 ml peqGOLD TriFast™ (VWR) followed by shaking for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Microbiology 2022Quote: ... to obtain a final concentration of 1 μM and sodium nitrate (SS0680-500GR VWR) to obtain a final concentration of 10 μM.
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bioRxiv - Biophysics 2022Quote: ... induced with 1 mM B-isopropyl-thiogalactoside-non-animal-origin (IPTG-NAO; VWR USA) in the early-mid log phase ca 1.5 h or OD600 of 0.6 and incubated for another 5 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... by injecting 50 μl of substrate buffer (lysis buffer+ 1 mM DTT (VWR Scientific), 1 mM ATP (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized by treatment with 250 µL PBS + 1 mM CaCl2 (0556, VWR), 1 mM MgCl2 (M8266 ...
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bioRxiv - Genomics 2022Quote: ... and was stopped by adding 1 μL of 18 mM EDTA (VWR, 97062-656) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were blocked for 1 h with Rockland blocking buffer (VWR, Catalog #RLMB-070) then incubated with PDI antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:50 in 5 % BSA/TBST overnight at 4°C (BSA: VWR; #422361V). As a negative control ...
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bioRxiv - Immunology 2023Quote: ... 1 × 107 hepatocytes were mechanically disrupted with a pellet mixer (VWR Radnor, PA, USA) for 5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: 1,5 x106 PBMCs were seeded in 1 ml RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction was quenched by adding 70 µL 1% formic acid (VWR, Radnor, PA) containing 0.1 µg/mL isotope-labelled peptide Ac-KKTIPNDSR*E (Cambridge Peptides ...
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bioRxiv - Systems Biology 2024Quote: ... Afterwards beads were rinsed twice with 1 mL 80% (v/v) ethanol (EtOH, VWR) and once with 800 µL ACN ...
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bioRxiv - Synthetic Biology 2024Quote: ... Supernatants were discarded and the pellets were then resuspended in 1 mL acetone (VWR), followed by adding 200 μL glass beads (Sigma) ...
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bioRxiv - Synthetic Biology 2024Quote: ... LB agar was prepared by dissolving 37 g L−1 LB agar powder (VWR) into required amount of distilled water ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellets were resuspended in 1X PBS containing 1% formaldehyde (AA33314-K2, VWR) and protease inhibitors ...
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bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... a part of the organ tissue was weight and homogenised following the protocol of the Precellys soft tissue lysing kit (CK14, Bertin Instruments, VWR, UK). The fluorescence of Cy7-psomes was measured in using a multimode microplate fluorometer (Spark ...
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bioRxiv - Genetics 2020Quote: ... centrifuged and the aqueous phase was applied to a PerfectBind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA). Further extraction of total RNA was performed according to the kits protocol ...
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bioRxiv - Physiology 2022Quote: ... The resulting aqueous phase was transferred to a Perfect Bind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA), following the kit’s protocol for purification ...
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bioRxiv - Molecular Biology 2022Quote: ... following the instructions of the manufacturer with minor modifications (according to Exiqon’s Guidelines for ISH on fresh frozen material using the miRCURY LNA ISH Optimization kits) and using Pertex (VWR, Stockholm, Sweden) for mounting ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... reverse transcription was performed using 4.0 μl of 10.0 μl total RNA solution with the cDNA-Synthesis Kit H Plus (VWR Peqlab, Germany, 732-3273). CFX Connect™ Real-Time PCR Detection System (BioRad ...
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... We then used a glass slide to touch each region of the inside of the reproductive tract and stained these slides with a modified Giemsa stain (Differential Quik Stain Kit, VWR, Radnor PA) to look for the presence of sperm and inflammatory cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% non-fat milk and 1% BSA filtered through a paper filter (qualitative, 415, VWR). Blots were rinsed 1-2 times with Wash Buffer ...
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bioRxiv - Immunology 2019Quote: ... DRGs were immediately digested with 1 mL of papain solution (HBSS/10 mM HEPES (VWR)/500 μM EDTA (Westnet)/0.4 mg/mL L-Cysteine (Sigma)/1.5 mM CaCl2 (Sigma)/20 unit/mL Papain (Worthington) ...
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bioRxiv - Biophysics 2021Quote: ... Two no.1 cover slides (24 × 60 mm and 18×18mm, VWR, Radnor, Pennsylvania, USA) are cleaned by sonication in isopropanol and dried under a stream of N22 ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
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bioRxiv - Cell Biology 2022Quote: Liver (∼100mg) was homogenized in 1 mL of PBS using a Bead Mill Homogenizer (VWR). Lipids were extracted ...