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Citations for VWR :
901 - 950 of 1779 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 2 µl were spotted on cellulose TLC plates (20 cm x 20 cm, VWR EM1.05718.0001) along with 10 µg of dNMP standards and developed in isobutyric acid:H2O:NH3 (66:20:1) ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of cells were transferred to 4.5 mL disposable cuvettes (VWR Cat# 30620-276) Ethidium bromide (6.25 μM ...
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bioRxiv - Genomics 2021Quote: ... and 5mL fractions were removed and filtered (70uM) into RPMI supplemented with 2% FCS (VWR) three times ...
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bioRxiv - Microbiology 2021Quote: ... using 2 mL of culture per treatment in plastic Falcon tubes (VWR, Cat. No. 352059). After antibiotic treatment was added ...
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bioRxiv - Bioengineering 2022Quote: ... The end of the PTFE tubing was placed within the 2% agarose hydrogel matrix (VWR Life Science ...
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bioRxiv - Cell Biology 2023Quote: ... The aliquots were individually heated at different temperatures (Doppio 2×48 well Thermal cycler, VWR). After the heat treatment ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... and spectinomycin (100 μg/mL) in a 2 mL 96 well deep-well block (VWR) sealed with breathable film (VWR ...
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bioRxiv - Cell Biology 2024Quote: Plated cells were fixed for 10 minutes with 2% paraformaldehyde (PFA; VWR Chemicals, Cat# 28794.364) in phosphate buffer and washed for 3×5 minutes with PBS ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2% FBS were injected into the left ventricle of the mice with a 26G × 3/8” needle attached to tuberculin syringe (VWR, Cat: BD309625). During the course of colonization assays ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-S100 1:300 (VWR/ProteinTech 15146-1-AP), Chick anti-Neurofilament 200 kDa 1:500 (Aves NFH) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked by 5% Powdered milk (Roth, T145.3) in PBS-0.2% Tween20 (VWR Life Science, M147-1L), detected with the indicated antibodies and analyzed by quantitative near-infrared scanning (Odyssey ...
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bioRxiv - Developmental Biology 2021Quote: ... for 5 minutes and washed in PBST for 25 minutes before mounting onto charged slides (VWR #48311-703) with Fluoromount (Electron Microscopy Sciences #17984-25) ...
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bioRxiv - Immunology 2019Quote: ... or with 5×104 B16F10 wild type (WT) or B16F10-Globin-SL8-intron cells along with matrigel (VWR). Five days after challenge ...
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bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were again washed in PBS for 6 × 5 mins before mounting onto polylysine-coated slides (Polysine, VWR). Mounted slices were left to dehydrate overnight before applying coverslips using either Aqua-Poly/Mount (Polysciences Inc. ...
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bioRxiv - Zoology 2021Quote: ... the samples were passed through a 5–15 μm pore size paper membrane (VWR, Cat No. 516-0813) by gravity filtration to avoid damaging the cells– M ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A 5 mL syringe was connected to a swivel using Tygon* tubing (AAQ04103; VWR; West Chester, PA, USA). Each swivel was coupled with a spring leash (C313C ...
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bioRxiv - Microbiology 2019Quote: ... Tissue cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, (VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Genomics 2022Quote: RNAscope assay was performed on lung 5 μm FFPE sections on Superfrost Plus microscope slides (#631-9483, VWR) cut from depths consecutive to the sections mounted on Visium slides ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed three more times with PBS and blocked in PBS 5% bovine serum albumin (BSA, VWR) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
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bioRxiv - Immunology 2023Quote: ... at a density of 5 x 106 cells/mL in 100 μL of RPMI 1640 (#45000-396, VWR) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was lysed with 500 μL of Cell Lysis Buffer [5 mM PIPES (VWR, 0169-100g), 85 mM KCl ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Microbiology 2019Quote: Colony morphology agar was prepared as described above and 4 mL of the mixture were poured into 35 mm×10 mm round Petri dishes (25373-041, VWR)and left to cool and solidify overnight (14 to 24 h ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma], pH 6.84, with KOH [VWR], 1mM EGTA [Sigma], 4mM MgCl2 [VWR]) to remove excess F-127 in solution and exchange buffers ...
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bioRxiv - Biochemistry 2020Quote: Frozen hippocampus brain tissues were homogenized in a 4 volume of the 10% trichloroacetic acid (VWR International, Radnor, PA, USA) solution using a Kontes glass tissue grinder ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
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bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Biophysics 2023Quote: HeLa cells were fixed using 4% paraformaldehyde and permeabilized using Triton-X100 (VWR, Radnor, PA, USA, Cat. No. M143-1L) 12 hours after TTLL6-YFP transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% DMSO (VWR) and 0.2 mM 1-phenyl-2-thiourea (Acros Organics ...