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Citations for VWR :
851 - 877 of 877 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and 4% TCA (100μl) were added to these tubes and vortexed for 1 minute on a multi-tube vortex mixer (VWR International LLC, Radnor, PA).
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bioRxiv - Neuroscience 2022Quote: ... mEPSCs were recorded at a holding potential of −70 mV in the presence of 50 μM PTX and 1 μM tetrodotoxin (TTX; VWR, Catalog#89160– 628). Electrophysiological data were analyzed using Clampfit 10.5 (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were mixed with the scintillation liquid and counted in a scintillation counter (Packard TriCarb Model, 1 min/vial) using Solvent-Saver™ scintillation vials (VWR International LLC.).
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (HeLa) or 15% (Tig-1) fetal bovine serum (HyClone, GE Healthcare Life Sciences, Marlborough, MA; Avantor Seradigm, VWR, Radnor, PA), 100 units/mL penicillin and 100 µg/mL streptomycin (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Immunology 2023Quote: ... The wells were emptied and blocked with 300 µL of PBS containing 1% BSA (Bovine Serum Albumin Fraction V, VWR Cat# 97061-416) for 1 h at 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
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bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
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bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... was mixed with deuterium oxide (450 µl) containing sodium formate (1 µmol/L) and then passed through a 10 KDa cutoff centrifugal filter (VWR, Radnor, PA, United States). 1H-NMR spectra were acquired using a 600 MHz spectrometer (Bruker AVANCE II ...
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bioRxiv - Synthetic Biology 2020Quote: ... For that purpose specially designed single-use imaging chambers were made using 18 mm × 18 mm cover glasses #1 (VWR, Cat No. 631-1331) and silicon seals cut out of ultra thin silicone film with 0.3 mm thickness (Silex ...
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bioRxiv - Microbiology 2019Quote: ... Colonies of each bacterial test isolate and a positive and negative control isolate were harvested with a 1 μl loop (VWR International, catalog # 12000-806) from blood agar (BD Diagnostics Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was allowed to cool at room temperature (RT) for 10 min before adding 1 µl RNAse at 10 mg/ml (VWR cat no. 0675-250MG) and incubation at 37 for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... Elma Schmidbauer GmbH), mixed (10 min, 2035 rpm, HeidolphTM MultiReax) and centrifuged (1 min, 12300 RCF, Micro-Star 12, VWR®, Radnor, PA, USA). The total protein concentration in each sample was determined using the BCA assay in 10-20 μl sample aliquots ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...
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bioRxiv - Microbiology 2024Quote: Fresh bacterial cultures were inoculated with the wastewater sample at a 1:10 ratio in a 96 deep well plate (VWR, cat. no. 10755-250). Any surface biofilms present following incubation were disrupted via agitation of the culture with plastic pipette tips ...
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bioRxiv - Cell Biology 2022Quote: ... then spheroplasted in digestion mix (8.9% glusulase [part no. NEE154001EA, PerkinElmer] and 1 mg/mL 100T zymolyase [cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR] in 1.2M sorbitol-citrate) by rotating at 30°C for 4-5 hours (digestion was assessed by looking at cells on a light microscope) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed 24h after transfection in lysis buffer (75 mM Tris-HCl [Sigma-Aldrich], 400 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals], pH 8) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Bioengineering 2022Quote: ... WM989 A6-G3 H2B-GFP and WM989 A6-G3 RC4 cells were mixed together and plated on coverslips (VWR, 16004-098, 24×050mm, No. 1 coverglass) with 24-well silicone isolators (Grace Bio-Labs ...