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Citations for VWR :
851 - 900 of 1252 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Before digestion the samples were diluted 1:2 with MS grade water (VWR). Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
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bioRxiv - Immunology 2022Quote: ... plates were blocked with 50 μL of 1% BSA (VWR, cat. #97061-422) in PBS for 1 hours at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044) were incubated 72 h at 27 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 50:1 mixture of XTT salt and PMS electron-coupling reagent (both VWR) was added to the wells and incubated for 4 h at 37 °C in 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... parasites were pretreated with 1 μM mycalolide B (VWR cat. no. 89165-002) in invasion media (sodium bicarbonate-free DMEM containing 20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... 0.33 mM of each dNTP and 1 U of Taq DNA polymerase (VWR) using one cycle of 94°C for 5 min 30 s ...
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bioRxiv - Cell Biology 2022Quote: ... covered with a glass coverslip (VWR Ltd, thickness No. 1, diameter: 22 mm) and sealed with glue (EVO-STIK ...
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bioRxiv - Physiology 2024Quote: ... tissues were homogenized in 1 mL of RiboZol (Cat no. N580, VWR, USA), and then total RNA was precipitated in 500 μL of isopropanol ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were rehydrated into PBS + 1% Triton X-100 (PBTr) (VWR #97063-864) and incubated with ice-cold acetone at -20C for 20 minutes ...
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bioRxiv - Physiology 2024Quote: ... Western blot secondary antibodies included: donkey anti-rabbit HRP (VWR 95017, 1:1000) and sheep anti-mouse (GE Healthcare #NA931V ...
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bioRxiv - Immunology 2024Quote: ... then blocked with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 hours at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were analysed on a 1-2 % agarose gel (VWR, #732-2789P) prepared in 1X TAE buffer (VWR ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were transferred a 1 mm-gap sterile electroporation cuvette (VWR, 732-1135) and electroporated using an ECM 399 Exponential Decay Wave Electroporation System (BTX ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old seedlings were placed in a 1-well chambered coverglass (VWR, Kammerdeckglä ser ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 mg/mL aTc stock was prepared by dissolving anhydrotetracycline hydrochloride (VWR, CAYM10009542) powder in 70% ethanol and stored at -20 °C in the dark.
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed in 1% (wt/vol) sodium dodecyl sulfate (SDS) (VWR, #97062) for 24 hours or until white transparent tissue was obtained ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% Pen/Strep and 10% FBS (VWR International, Radnor, PA, #97068-085). For LLOMe treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... and exposed for 1–3 min to maximum sensitivity film (VWR, Radnor, PA). Broad-range pre-stained SDS–PAGE molecular weight markers (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed in 1% (wt/vol) sodium dodecyl sulfate (SDS) (VWR, #97062) for 24 hours or until white transparent tissue was obtained ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and cDNA was synthesized with the peqGOLD MicroSpin Total RNA Kit (Peqlab/VWR Life Science, 12-6831) and the qScript™ Flex cDNA Kit (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Molecular Biology 2021Quote: ... 1ug of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 1µg of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Tissues were homogenized with a Minilys homogenizer (Bertin Technologies, Rockville, MD) using a Precellys Lysing Kit (VWR Scientific, Radnor, PA). Total RNA was extracted from thoroughly homogenized tissues via miRNeasy Mini Kit (#217004 ...
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bioRxiv - Immunology 2020Quote: ... Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069 ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA used for gene expression analysis was purified using Zymo Research Direct-zol RNA Miniprep Plus kit (VWR 76211-340) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted and purified using RNA cleanup columns (IBI scientific TRI-Isolate RNA Pure Kit, VWR cat# 76006-526), eluted into 25 μL of water and immediately visualized by ge (see “Gel-based visualization of RNA”)
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bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... a part of the organ tissue was weight and homogenised following the protocol of the Precellys soft tissue lysing kit (CK14, Bertin Instruments, VWR, UK). The fluorescence of Cy7-psomes was measured in using a multimode microplate fluorometer (Spark ...
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bioRxiv - Genetics 2020Quote: ... centrifuged and the aqueous phase was applied to a PerfectBind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA). Further extraction of total RNA was performed according to the kits protocol ...
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bioRxiv - Physiology 2022Quote: ... The resulting aqueous phase was transferred to a Perfect Bind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA), following the kit’s protocol for purification ...
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bioRxiv - Molecular Biology 2022Quote: ... following the instructions of the manufacturer with minor modifications (according to Exiqon’s Guidelines for ISH on fresh frozen material using the miRCURY LNA ISH Optimization kits) and using Pertex (VWR, Stockholm, Sweden) for mounting ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... RPE-1 cells were incubated in medium containing 356 µM cycloheximide (VWR Chemicals, 441892A) for 6h.
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bioRxiv - Cell Biology 2020Quote: ... Expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, 437144N) at 26 °C for 6 h ...
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bioRxiv - Microbiology 2020Quote: ... or after 10-1 dilution in molecular biology grade ddH20 (VWR Chemicals, Darmstadt, Germany) for specific amplification and high throughput sequencing of the fungal rDNA internal transcribed spacer 1 (ITS1) ...
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bioRxiv - Microbiology 2019Quote: ... 1 U of VWR Taq DNA Polymerase (VWR®, WR International bvba/sprl, Belbium), 1 X key Buffer supplemented with MgCl2 (1.5 mM) ...
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bioRxiv - Bioengineering 2020Quote: ... Both methods of surface modification were applied to 1” x 3” glass slides (VWR). A small volume (1.75 µL ...
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bioRxiv - Neuroscience 2020Quote: ... cooled down with −1 °C/min in a cryo container (Mr. Frosty™, VWR) and stored at −80 °C or in liquid nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... an equal volume of 25:24:1 phenol-chloroform-isoamyl alcohol (VWR, Radnor, PA) was added and the tubes were vortexed for 30 s followed by centrifugation at 10,000g for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by a washing step with 3 mL 1% acetonitrile (ACN; VWR, 83640-290)/0.1% TFA and elution with 500 µL 40% ACN and 500 µL 60% ACN ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked at room temperature for 1 h using Rockland blocking buffer (VWR). Primary PDI antibody (Abcam ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed after 48h with 1% sodium dodecyl sulfate (SDS) in PBS (VWR), then proteins were separated on 4-12% Mini-PROTEAN GTX polyacrylamide gel (Bio-rad ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Colon tissues were homogenized for 1 minute using a disperser (T10 ULTRA-TURRAX; VWR) on ice ...
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bioRxiv - Microbiology 2019Quote: ... A total of 1 x 108 CFU was added to fresh sheep blood (VWR) in V-bottom 96-well plates (Corning) ...