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Citations for VWR :
801 - 850 of 976 citations for Proteasome 26S Subunit Non ATPase 10 PSMD10 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cell lines were obtained and cultured as previously described (7) in RPMI-1640 basal media supplemented with 10% (15% for U266B1 cells) fetal bovine serum (FBS, VWR) with 1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... CSF samples were diluted 10-fold in 0.02 µm filtered PBS and added to the glass cover slip (VWR, 6310135) and incubated for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Washing was carried out three times for 10 min at room temperature with TBS + 0.1% Tween 20 (VWR, United States) and the membrane was incubated with appropriate fluorescent secondary antibodies (listed in Supplementary Table S1 ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were cut at a thickness of 10 μm using a Leica CM1950 cryostat and placed onto SuperFrost Plus slides (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Embedded tissue blocks were sectioned on a cryostat in slices 10 to 20 micrometers thick and attached to Superfrost Plus microscope slides (631-0108, VWR). The sections can be stored at −80 indefinitely ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Pathology 2024Quote: ... Reverse transcription was performed for 30 min at 42 °C using dNTP Mix (10 mM each, VWR, Radnor, PA, USA) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... the inactivating solution was replaced by pre-warmed plating medium (10% horse serum (#S900-500, VWR International GmbH, Darmstadt, Germany), 1.8 mM glutamine ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions containing SA1 were pooled concentrated to 1ml final volume and was purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Cell Biology 2022Quote: ... HAF were seeded onto Ø 10 or 16 mm borosilicate glass cover glasses (631-0170 and 631-0151, VWR, Germany). After transfer into 12- or 24-well plates (665102 and 662160 ...
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bioRxiv - Immunology 2023Quote: ... After the 10 minutes the reaction were stopped with 100 μL 0,5 M sulphuric acid (VWR Chemicals, cat no: 30144.294). The plates were read at 450 nm on an EL808 Ultra Microplate Reader (BioTek Instruments)
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bioRxiv - Bioengineering 2023Quote: ... the pigs were euthanized with intravenous barbiturate and the graft was excised and flushed with 300 mL PBS followed by 300 mL 10% neutral buffered formalin (NBF, VWR). If the kidneys could not be fully flushed due to thrombosis ...
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bioRxiv - Microbiology 2024Quote: ... The resulting monolayer of cells was isolated and washed using RPMI1640 (Stemcell, #36750) supplemented with 10% FBS (VWR, #97068-085), Pen/Strep (Thermo ...
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bioRxiv - Synthetic Biology 2024Quote: The variant BUD-ELMs were grown in standard conditions and then collected into a 1.5 ml Eppendorf tube and spun for 10 s at 3200 rcf using a mini centrifuge (VWR - C0803). Then the supernatant was removed ...
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bioRxiv - Neuroscience 2024Quote: Frozen tissue was briefly thawed and mechanically lysed by addition of 100 μL lysis buffer (5% SDS, 50 mM Tris, pH 7.55 with HCl) and 10 ‘strokes’ with a pestle (VWR International) after which the pestle was washed with 100 μL additional lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were pelleted at 3200 x g for 10 minutes at room temperature then washed in 1X volume blood bank saline (89370-096, VWR) and resuspended in 7H9 + 0.5% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... Cryosections were then cut at a thickness of 10 μm using a Leica CM1950 cryostat and placed onto SuperFrost Plus slides (VWR) for ISS or Visium CytAssist ...
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bioRxiv - Cell Biology 2024Quote: ... and 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FB Essence (FBE, VWR, #10803-034) and penicillin/streptomycin (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... then pelleted at 3200 x g for 10 minutes at 4°C and washed twice with pre-chilled blood bank saline (89370-096, VWR). Pellets were resuspended in 0.8 mL 3:1:0.004 acetonitrile:methanol:formic acid + 10 nM verapamil (V105 ...
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bioRxiv - Cancer Biology 2024Quote: ... epithelial-stromal composites were incubated for 30 min in permeabilisation buffer (PB1; 0.5 % bovine serum albumin (VWR International, 126575-10), 0.25 % fish-skin gelatin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: One million viable cells (≥ 10 µm diameter) were plated (N0) into uncoated 3.5 cm Falcon™ plastic tissue culture dishes (Corning/VWR) containing 2 mL arginine-free Dulbecco and Vogt’s Modified Eagle’s Medium (standard plating conditions) ...
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bioRxiv - Bioengineering 2024Quote: ... We used a 1 : 10 ratio of tissue to deionized water and homogenized the tissue samples in a bead-mill homogenizer (VWR). We then diluted the tissue homogenates 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Neuroscience 2021Quote: ... They were treated to a 1:1 mixture of 100% ethanol and methyl salicylate for 10 minutes before clearing and storage in methyl salicylate (VWR Chemicals).
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bioRxiv - Cell Biology 2020Quote: ... which was mixed thoroughly and allowed to stand for 10 minutes prior to filtration through a 0.2μM vacuum filter unit (VWR, Cat # 10040-460) to remove particulates ...
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was suspended in 1 ml PBS at room temperature (POPC) or 55 °C (DPPC) and then sonicated for 10 minutes in an ultrasound bath (USC500TH, VWR, England) at the same temperature to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Bioengineering 2020Quote: ... of BIO-133 or 10% PEG-DA (ESIBIO GS705) on a #1.5 cover glass (24 mm x 50 mm, VWR, 48393-241). The droplet was positioned between two 100 μm spacers (Precision Brand ...
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bioRxiv - Biochemistry 2021Quote: ... FOCUS and HuH-7 cells were grown in Dulbecco’s minimum essential medium (DMEM) supplemented with 10% FBS (VWR Life Science, Seradigm). C3A ...
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bioRxiv - Microbiology 2020Quote: All strains were cultured for 10 hours with aeration at 37°C in brain heart infusion (BHI; VWR, Radnor, PA, USA) medium ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Cell Biology 2020Quote: Mid third-instar larval (L3) brains and imaginal discs were dissected in fresh Phosphate-Buffered Saline 10X (PBS, VWR #L182-10) and fixed for 30 minutes (min ...
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bioRxiv - Synthetic Biology 2022Quote: ... An approximate volume of 100-200 µL of BUD-ELMs were collected into a 1.5 mL centrifuge tube and spun for 10 sec at 3200 rcf with a mini centrifuge (VWR®, C0803). This allowed for the material to collect at the bottom as an homogeneous paste ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... The spacers supported the top 24×40mm #1 (0.13-0.16mm) coverslip (Knittel Glass) placed over the pupae and covered with a fine layer of mineral oil 10 S (VWR International). The top coverslip was glued to the spacers using nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Immunology 2020Quote: ... equal numbers of cells were counted by Moxi Mini automated cell counter (Orflo) and 0.6×10^6 cells per data point were seeded in T-25 cell culture flasks (VWR, PA, USA) in appropriate volume of culture medium ...
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bioRxiv - Microbiology 2020Quote: ... then boiled for 10 minutes or placed in an uncovered polystyrene petri dish (60 mm x 15 mm, VWR, Radnor, PA) for exposure ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Developmental Biology 2021Quote: Wandering third-instar larval (L3) brains and imaginal discs were dissected in fresh Phosphate-Buffered Saline 10X (PBS, VWR #L182-10) and fixed for 30 minutes (min ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Microbiology 2020Quote: ... and sonicated for 15 min in a water bath before transfer to a 10 kDa molecular weight cut-off filter (VWR Scientific). After 15 min of centrifugation at 14,000 × g (20°C) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in DMEM (PAN-Biotech GmbH, Aidenbach, Germany) supplemented with 10% (v/v) fetal bovine serum (VWR International GmbH) and 0.2% penicillin-streptomycin (PAN-Biotech) ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were immersed in a mixture of 100% ethanol and methyl salicylate (1:1, 10 minutes) before being stored in methyl salicylate (VWR Chemicals). Some slices were stored in a cryoprotective solution containing glycerol (20% ...
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bioRxiv - Immunology 2023Quote: ... Core temperature was measured at 10-minute intervals for 40 minutes post- challenge using a rectal probe (VWR International, 23226-656). Hemoconcentration was measured using the HemataSTAT II centrifugation device (VWR International ...
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...