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Citations for VWR :
801 - 850 of 1051 citations for 5 Cyano 2 3 bis 3 cyanophenyl 2H tetrazolium chloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... blood was washed out with 2 ml PBS and 1 ml of latex dye (VWR, LL0057-1L) was slowly and steadily injected into the left ventricle with an insulin syringe ...
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was then normalized to 2 ng/µL by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot.
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bioRxiv - Microbiology 2020Quote: ... cells were maintained in Joklik’s modified Eagle’s minimal essential medium (US Biological, M3867) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085), 2 mM l-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... concentrated OMVs were diluted 4 times in dH2O and 5 μL of this dilution was added to 150 μL Bradford reagent (VWR). Absorbance was measured at 595 nm and compared to a standard curve to calculate protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... The strains were first passed from agar plates (stored at 4oC) to 5 ml medium (round bottom polystyrene tubes, VWR) until early stationary phase ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 in a 96-well U-bottom plate (Falcon-Corning U-bottom tissue culture plates, VWR, Radnor, PA, USA). In conditions where rhCD80-Fc (Abatacept ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was filtered through a 35µm cell strainer into a 5 ml falcon round bottom tube (VWR #21008-948) for sorting using a MoFlo XDP cell sorter fitted with a 100µm nozzle ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween20) and were fixed in egg buffer with 1% paraformaldehyde for 5 minutes on a Superfrost Plus slide (VWR). Gonads were then flash frozen in liquid N2 and the cover slip was removed ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) and were mounted onto Superfrost Plus microscope slides (VWR) in VECTASHIELD antifade mounting medium (Vector Laboratories) ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Microbiology 2021Quote: L929 cells were grown in either suspension or monolayers in Joklik’s modified Eagle’s minimal essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085). CHO cells were grown in Ham’s F12 medium (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... were washed with 5% HellmanexIII (Lactan, 105513203) in ≥18 MΩ MonoQ H2O at 65°C in a table top Incu-Line oven (VWR) for 4 h and then rinsed 10 times with ≥ 18 MΩ MonoQ H2O ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated for 72 hours at 37°C in 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). All wells had a DMSO concentration of 0.5% v/v with drugs MRX-2843 (25 – 800 nM) ...
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bioRxiv - Microbiology 2022Quote: ... after electrophoresis gels were immersed into a methanol/sodium acetate (pH=5) solution and incubated at room temperature on rocking platform (VWR) at 1 speed for 2 min with constant shaking ...
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bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes prior to incubating for 72 hours at 37°C in a 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). Following incubation ...
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bioRxiv - Plant Biology 2023Quote: ... 4-day old Col_0 seedlings were incubated with LRC liquid medium supplemented with indicated concentrations of compounds (Adenosine 5′-triphosphate magnesium salt, ATP-Mg [VWR] ...
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bioRxiv - Bioengineering 2023Quote: ... the 3D printed PCL scaffolds were cut into dimensions of 5 mm × 15 mm using a surgical blade (VWR, Germany) and detached from the glass substrate by 70% EtOH (PanReac AppliChem ITW Reagents ...
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bioRxiv - Cancer Biology 2023Quote: ... purified cells/cell aggregates were washed in PBS and frozen in 5% dimethyl sulphoxide (DMSO; 23486.297, VWR, Radnor, PA, USA) in FBS until xenografting.
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... gels were immersed in a methanol-sodium acetate solution (pH 5) and incubated at room temperature on a rocking platform (VWR) at a speed of 1 for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were then irradiated with UV-A and UV-B for one hour a day for 5 consecutive days [42] in UV crosslinker (cat. no: 89131-486, VWR). On each exposure ...
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bioRxiv - Synthetic Biology 2023Quote: ... We grew the transformed library in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Neuroscience 2024Quote: ... larvae aged 5 dpf were transferred to individual wells of a 24-well plate at ZT7–8 (VWR, 82050-892), and acclimated overnight to the testing environment ...
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bioRxiv - Neuroscience 2024Quote: Frozen tissue was briefly thawed and mechanically lysed by addition of 100 μL lysis buffer (5% SDS, 50 mM Tris, pH 7.55 with HCl) and 10 ‘strokes’ with a pestle (VWR International) after which the pestle was washed with 100 μL additional lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Blots were washed with 1x TBS with 0.1% Tween-20 (TBST) and blocked using 5% IgG-free BSA (VWR RLBSA50). Primary antibodies (Supplemental Table 2 ...
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bioRxiv - Physiology 2024Quote: ... MPCs were allowed to differentiate for 5 days before adding differentiation medium supplemented with 50 nM 4-OH TAM (89152-604, VWR) for 48 hrs to induce recombination in HSA-miR-1-derived myotubes (or for TAM control in WT-derived myotubes) ...
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bioRxiv - Biochemistry 2024Quote: ... 200-300 million K-562 cells were collected by centrifugation (5 min at 200 x g, Mega Star 600 R centrifuge (VWR), rotor #75005701 (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... pHrodo™ iFL Red STP Ester was diluted to 2 mM in DMSO (VWR Life Science, 97063-136). Antibody was brought up in DPBS (2 mg/mL final concentration for Dll4 polyclonal antibody [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Trifluoroacetic acid, acetonitrile and 2-propanol were from Biosolve BV (Valkenswaard, The Netherlands) and glycine was from VWR International (Radnor ...
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...