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Citations for VWR :
801 - 850 of 998 citations for 1 Bromo 3 difluoromethoxy benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Neuroscience 2024Quote: ... S3: the lysate was extracted with an equal volume of phenol:chloroform:iso-amyl alcohol (25:24:1) (VWR, 97064-692), and the upper aqueous phase was then precipitated from ethanol.
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bioRxiv - Biochemistry 2024Quote: ... 500 µL of the upper phase was transferred in a new tube containing 1 mL isopropanol (VWR, 84881-320) and 2 µL f 15 mg/mL Glycoblue (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... adjusted for 1 hour in a 50:50 solution of 30% sucrose solution: Optimal Cutting Temperature (OCT) compound (VWR), followed by 1 hour in 100% OCT and snap-frozen in OCT using dry ice ...
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bioRxiv - Microbiology 2024Quote: 100 μl of cell suspension at OD 0.7 were added to 1 ml of fresh GCB medium in a 6-well plate (VWR) and incubated at 37 °C with 5% CO2 for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was immediately and gently mixed with 1 ml NCM medium containing 0.38 M mannitol and recovered in a round bottom tube (VWR) at 37 °C on a rotary shaker (120 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... myofibril pellets were resuspended in 300 μL of ice-cold wash buffer (Buffer 2: 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 1 mM DTT; all chemicals from VWR), and tubes were centrifuged at 3,000 g for 10 minutes at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted fractions were pooled and further purified on a 1 ml HiTrap FF Q column (VWR 17-5053-01) using an AKTA FPLC system (Cytiva) ...
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bioRxiv - Synthetic Biology 2024Quote: HEK293T cells were seeded 1 day before transfection at 100,000 cells/well in 24-well plates (Multiwell cell culture plates, VWR) for the transient transfections ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 20% glycerol, 1 mM DTT, 50 mM spermidine; all chemicals from VWR), and stored at -80°C for analyses described below.
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bioRxiv - Cell Biology 2024Quote: For indirect immunofluorescence experiments cells were trypsinized and transferred either to Ø 18 mm cover glasses (No. 1; VWR) in 12-well tissue culture plates (TPP ...
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bioRxiv - Microbiology 2024Quote: ... The aqueous phase containing the DNA of each sample was transferred to a fresh conical tube prepared with 1/10th volume of 3M sodium acetate pH 5.2 (3032-16, VWR) and 1 volume of isopropanol (3032-16 ...
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bioRxiv - Neuroscience 2024Quote: ... O-1-) on the BD FACS ARIA III into standard sorting collection buffer (PBS + 10% FBS (VWR 89510-188)) ...
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bioRxiv - Physiology 2024Quote: ... and Alexa Fluor® 488 AffiniPure Don-key Anti-Mouse IgG (H+L) (Cat #102650-156, 1:300, VWR). The analysis of GR translocation was carried out using ImageJ software on 5-6 images per sample acquired from a Nikon Eclipse Ti-U microscope.
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bioRxiv - Neuroscience 2024Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1 hour each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Bioengineering 2024Quote: ... and blocked and permeabilized overnight at 4°C in retina blocking buffer (RBB) containing PBS with 1% BSA (VWR) and 0.5% Triton X100 (Sigma ...
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bioRxiv - Biophysics 2024Quote: Glass-bottom 384-well plates (Brooks, MatriPlate MGB101-1-2-LG-L) and cover glass (VWR, USA, 48393-251) were passivated with PEG-silane (MW = 5 kD ...
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bioRxiv - Biophysics 2024Quote: ... The collector was changed to a flat aluminum plate with a glass cover (#1 micro cover glass VWR Scientific) placed on the top ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg RNA from each condition was used to generate cDNA with qScript cDNA SuperMix (VWR Cat#101414-104) according to manufacturing protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary PSCs were seeded in 6 well dish at 1 x 104 confluency in Iscove’s modified Dulbecco’s medium (IMDM; Cytiva, SH30228.02) containing 10% FBS (VWR, 97068-085) and 1% Antibiotics-Antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...
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bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% Fast green (Raymond A Lamb) (both in distilled water) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Biochemistry 2022Quote: ... Cells on D-poly-Lysine coated coverslips were then fixed with pre-warmed 3.7% formaldehyde (v/v; VWR, VW3408-1), permeabilized with ice-cold methanol ...
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: A Ninjaflex 2 mm thick gasket was 3D printed onto a 50 mm × 75 mm × 1 mm glass slide (VWR) using a Makerbot 2× printer (Appendix I) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Systems Biology 2022Quote: Samples were grown using SD-His+G418 agar and medium selecting for the duplicated chromosomes (6.7 g/L yeast nitrogen base without ammonium sulfate, Difco Cat#233520; 20 g/L glucose, Sigma Cat#Y0626; 1 g/L monosodium glutamate, VWR Cat#27872.298 ...
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bioRxiv - Immunology 2022Quote: Bone marrow isolation and cell staining was performed on ice in staining medium (SM; Hanks Balanced Salt Solution [HBSS 1x, Corning 1-022-CM] supplemented with heat inactivated fetal bovine serum [FBS, VWR 97068-05] to a final concentration of 2%) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50 μL of 1% (w/v) sodium dodecyl sulfate (SDS, VWR International, Radnor, PA, USA). Colorimetric absorbance at 414 nm was measured using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...