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Citations for VWR :
751 - 800 of 968 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 2 µL cell suspension was loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5).
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bioRxiv - Biophysics 2023Quote: ... The cells were then washed with PBS and permeabilized with a 0.5% Triton-PBS (VWR, 0694-1L) solution for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and tissue was mechanically disrupted by beads to further lyse bacterial cell walls (VWR Mini Bead Mill).
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Total RNA from cells was isolated using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to the manufacturer’s protocol and RNA concentrations of the samples were determined with a NanoDrop 1,000 spectrometer (VWR ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were seeded at 225,000 cells/well onto 0.01 mg/mL poly-D-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: Hippocampal neurons seeded at 180,000 cells/well onto 0.1 mg/mL poly-L-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Developmental Biology 2023Quote: Cells were lysed after 12 h or 16 h using 500 μL pepGOLD TriFast ™ reagent (VWR) and stored at -80 until the RNA extraction process was resumed ...
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transferred to FACS buffer (2mM EDTA, 0.5% BSA in DPBS) and filtered (VWR, 734-0001). For single cell RNA sequencing ...
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bioRxiv - Microbiology 2023Quote: Cells were grown to mid-log phase in SC medium in 96 deep-well plates (VWR, 82051). After washing with ddH2O twice and spinning down at 3,000g for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated for 4 h with 4HC or doxorubicin at IC50-range drug concentrations using cells seeded the prior day at 50,000 (E0771) or 75,000 cells per well (4T1, B16F10) of a 6-well plate (cat. #10861-696, VWR). Cells were then washed once with PBS and incubated in fresh media for a total of 24 ...
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bioRxiv - Genetics 2020Quote: ... the egg-containing plug-in cells were removed from the plates and fixed vertically on petri dishes (VWR) with plastiline (Pelikan Schindellegi ...
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bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Cell Biology 2019Quote: ... Equal spotting was controlled by simultaneously spotting cells using a multi-channel ultra-high-performance pipette (VWR International). Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD) ...
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bioRxiv - Immunology 2019Quote: ... or with 5×104 B16F10 wild type (WT) or B16F10-Globin-SL8-intron cells along with matrigel (VWR). Five days after challenge ...
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bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
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bioRxiv - Cell Biology 2021Quote: An early passage iMPC clone was trypsinized and filtered using a 40 μm cell strainer (VWR, 734-0002) to filter out debris and fragments of myofibers ...
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bioRxiv - Microbiology 2020Quote: ... The flow cell was pre-incubated with microscopy medium and a bottom coverslip (25 mm No. 1.5; VWR) was attached to the cell ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were then washed 1x with PBS and resuspended in RIPA lysis buffer (VWR, cat # 97063-270) supplemented with protease (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 104 target cells were pipetted per well into 96-well black plates with clear-bottom (VWR/Corning) and kept in the dark for 20 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... For TPE-FLIM imaging the cells were seeded on 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ at room temperature.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then washed with PBS thrice and treated with 1.6 mM sodium metaperiodate (VWR, 13798-22) for 20 minutes at 4C for oxidation of the N-linked sugar residues ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with methanol for 5 seconds and stained with Giemsa (1:10 dilution with MiliQ, VWR) for 17 min ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated off and the cells were resuspended in 500 μl RiboZol RNA extraction reagent (VWR). Tubes were then vortexed and 100 μl chloroform was added to the tube ...
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bioRxiv - Plant Biology 2022Quote: ... The latter was captured in a 300 mL beaker and poured through a cell strainer (VWR, Radnor, USA) with a nominal pore size of 100 µm ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded on a 24-well plate on 12 mm #1.0 glass coverslips (VWR, CAT# 631-1577P) in 500 µL media ...
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bioRxiv - Neuroscience 2023Quote: ... The solution containing dissociated neurons was passed through a 100 µm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Cell Biology 2023Quote: ... Swollen cells were then ruptured using pre-cooled dounce homogenizer and the tight pestle (VWR Cat. 62400-595). The ruptured cells were centrifuged at 218xg for 5 min to pellet nuclei ...
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bioRxiv - Immunology 2023Quote: ... at a density of 5 x 106 cells/mL in 100 μL of RPMI 1640 (#45000-396, VWR) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Digested tissues were passed through 100 and 70 um nylon cell strainers (Corning, VWR# 10054-458, 89508-344). Single cell suspensions were washed by MACS buffer ...
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bioRxiv - Cell Biology 2023Quote: Frozen lentiviral stocks were generated by transfecting 70% confluent HEK-293T cells in 15cm cell culture dishes in ‘MEF medium’ together with a solution consisting of 1ml 150mM NaCl (1.06404.1000, VWR), 1ml Polyethylenimine (23966-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Cells and tissues were lysed by radioimmunoprecipitation assay (RIPA) buffer (0.2% IGEPAL CA-620, 0.02% SDS with VWR Life Science Protease Inhibitor Cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were separated from the culture liquid by centrifugation and filtration through 0.22 µm membranes (VWR, USA). Next ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in 6-well plates after placing round coverslips in the bottom of the wells (VWR), or in PhenoPlates 96-well (Perkin Elmer) ...
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bioRxiv - Neuroscience 2024Quote: ... The solution containing dissociated neurons was passed through a 100 μm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 1 hour in 2 ml of 3% BSA in PBS (VWR, cat#: 97063-624). Cells were then washed three times with 2 ml of PBS for 5 minutes each and mounted with Prolong Diamond Antifade Mountant (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... INS-1 β-cells were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 30 min ...
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bioRxiv - Biophysics 2024Quote: Experiments were done using a flow cell assembled by separating silanized hydrophobic coverslips and cleaned glass slides (VWR) by double sided tape (3M) ...