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Citations for VWR :
701 - 750 of 955 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Bioengineering 2024Quote: ... and brain and cardiac organoids were tested in 1×phosphate-buffered saline (PBS) (VWR international, 97063-660). Samples were secured using custom 3D-printed parts specifically designed to fit the sample shape and maintain stability during measurements ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 ml cultures were grown overnight and used to inoculate 1 L of terrific broth media (VWR). Cells were grown until they reached an OD of 0.8 and then cooled on ice before transferring to a 16 °C incubator for overnight induction using 0.5 M IPTG (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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bioRxiv - Developmental Biology 2022Quote: ... The agarose cubes containing forelimbs were submerged in 1 mL solution containing 0.05% sodium dodecyl sulfate (SDS; VWR), with 2% penicillin-streptomycin or 0.02% sodium azide (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Plant Biology 2020Quote: ... The AIR was digested with 1 mL ultrapure 70% nitric acid (BDH Aristar® Ultra, VWR, Radnor, PA) in 15 mL Teflon beakers ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... through sieves with different mesh sizes (4, 3.15, 2.5, 1.25 and 1 mm, VWR International GmbH, Darmstadt/Germany). Flowthrough of the 1 mm sieve was collected in a pan ...
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bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... eyes enucleated and fixed for 1 h in 4% paraformaldehyde (CAS: 50-00-0, Cat.No.: 100504-858, VWR)) in PBS at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... which consisted of RPMI-1640 medium supplemented with 1% Ultra Low IgG Fetal Bovine Serum (VWR 4100-050) and added to the cell-containing wells ...
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Blocks of around 1 mm2 were cut and cryo-protected by overnight infiltration in 2.3 M sucrose (VWR) in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We filter-sterilized the supernatants by transferring them to 96-well 1 mL filter-plates (VWR International, Switzerland), followed by centrifugation at 1200 rpm for 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then permeabilized in 1× PBS with 0.25% TritonTM X-100 (Cat. No. 97063-864, VWR) and FBS for 20 min ...
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bioRxiv - Biochemistry 2020Quote: α-Syn (1 mg/mL) was dissolved in 10 mM NaOH and sonicated (VWR® Symphony™ Ultrasonic Cleaner) for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with methanol for 5 seconds and stained with Giemsa (1:10 dilution with MiliQ, VWR) for 17 min ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 1 hour in 2 ml of 3% BSA in PBS (VWR, cat#: 97063-624). Cells were then washed three times with 2 ml of PBS for 5 minutes each and mounted with Prolong Diamond Antifade Mountant (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... INS-1 β-cells were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... mice were weighed 1 hr prior to harvest and injected with 0.04 µmol/g puromycin (VWR, #75844-852).
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of conditioned medium was combined with 395 μl of premixed TCA (256 µl VWR Life Science) and 1% Triton-X 100 (139 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... xaudsem is the binary semantic feature encoding auditory semantics (AN = 1, VN, VWR, AWR = 0, see Fig. 2A). xvissem is the binary semantic feature encoding visual semantics (VN = 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR). Proteins were then electrotransferred onto pre-activated polyvinylidene difluoride membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was extracted against an equal volume of phenol:chloroform:iso-amyl alcohol (25:24:1) (VWR, 97064-692). The upper aqueous phase was then precipitated from ethanol as described above ...
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bioRxiv - Neuroscience 2021Quote: Tissue samples were lysed in NP40 buffer (1% NP40 in 10 mM Tris, pH 7.4, 150 mM NaCl) in a Precellys homogenizator (VWR) and 10 μg total protein per sample were proteolyzed with Lys-C and trypsin using a modified FASP procedure (71).
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bioRxiv - Neuroscience 2021Quote: ... the samples were resolubilized with 15 min sonication in 30 μL of 1% acetonitrile + 0.1% trifluoroacetic acid in LC-MS grade water (all from VWR). The injection volume (between 2 and 10 μL ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.1% Fast green (Raymond A Lamb) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Immunology 2021Quote: ... and sequentially washed with RIPA (PBS pH 7.4 with 0.5% sodium deoxycholate [Thermo], 0.1% sodium dodecylsulfate [Fisher Scientific, Waltham, MA], 1% Nonidet P-40 substitute [VWR]), high salt PBS (PBS pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were rocked every 15 minutes for 1 hour before a semi-solid overly containing 0.5% Methylcellulose in viral growth media (VWR) was applied ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... we added 40 mg of powder yeast extract g−1 dry soil as a C and nutrient source (VWR). We chose yeast extract over glucose to provide more varied forms of C and thus stimulate more diverse microorganisms (Fierer et al. ...
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bioRxiv - Pathology 2022Quote: ... Color development was obtained by incubation with KPL Sure Blue TMB Microwell Peroxidase Substrate 1-Component (95059-286, VWR), and the reaction was stopped using 2M H2SO4 (80012010.2500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were serum-starved 16 hours prior to plating with 1% bovine serum albumin (VWR, Cat. no. 0332-100G) added for protein supplementation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were run on 7.5-12% polyacrylamide gels for ∼1 hour at 150V and transferred to PVDF membranes (VWR) for 1 hour at 330 mA on ice ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Bioengineering 2022Quote: ... Optical density (OD) was measured in technical triplicates in 1-mL polystyrene cuvettes in a V-1200 spectrophotometer (VWR) at 600 nm ...
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bioRxiv - Immunology 2022Quote: ... Cell density was adjusted to 1×106 cells/ml and added to the FACS tubes (Falcon® Brand, VWR). After pelleting ...
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bioRxiv - Microbiology 2022Quote: ... was diluted 1:3 in cold PBS and 100 μL was added to 0.4 μm polycarbonate trans-well plate inserts (VWR). The Transwell plates were placed 4°C for 30 min to let the Matrigel settle and were then moved to an anaerobic chamber to gel at 37°C for 24 h ...