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Citations for VWR :
701 - 750 of 1631 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 50 ng/ml recombinant murine macrophage-colony stimulating factor (rmM-CSF, Genentech)] and plated in 15-cm non-TC treated dishes for 5 days (Petri dish, VWR, 25384-326). Fresh BMDM media was added on day 3 without removal of original media ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.4 μl of each primer and 5 μL VWR Red Taq DNA polymerase Master mix (VWR Chemicals, Denmark, Cas nr: 733-2547). The PCR reaction conditions for amplification of DNA were as follows ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were stopped by incubating on ice for 5 min and digesting with 1 µg of Proteinase K Solution (VWR, PA, USA) for 15 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA/DNA hybrid was tagmented in TD reaction buffer (10 mM Tris-Cl pH 7.6, 5 mM MgCl2, 10% DMF) supplemented with 3.4% PEG8000 (VWR Life Science, Cat.No.97061), 1 mM ATP (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... decalcification solution for 14 days and then paraffin embedded for sectioning with a Leica RM2235 microtome at 5 μm per section to generate histology slides (VWR 631-1553). Sections were processed and stained with TRAP solution and counterstained with 0.2% methyl green solution (Bioenno Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... Individual yeast colonies were picked into 4mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
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bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
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bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biophysics 2023Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Physiology 2021Quote: Paraformaldehyde (4%; VWR) fixed platelets were centrifuged onto poly-L-lysine coverslips (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... once and fixed using 4% formaldehyde (Thermo Fisher)/4% sucrose (VWR) in 1x PBS for 15 minutes at room temperature ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... / 4% sucrose (VWR) in 1X PBS for 15 min ...
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bioRxiv - Biochemistry 2019Quote: ... 1 x 106 cells were pelleted and resuspended in a 1:2.5:1 mixture of H2O: methanol: chloroform (both VWR, Radnor PA, USA) pre-cooled to −20 °C ...
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bioRxiv - Microbiology 2019Quote: Colony morphology agar was prepared as described above and 4 mL of the mixture were poured into 35 mm×10 mm round Petri dishes (25373-041, VWR)and left to cool and solidify overnight (14 to 24 h ...
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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bioRxiv - Microbiology 2019Quote: ... Aliquots of the diluted culture (100 mL/well) were incubated in five 96-well flat bottom polystyrene dishes (VWR) for 0 ...
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bioRxiv - Bioengineering 2022Quote: ... tissue culture 96-well plates (Coastar) were pre-treated with 100 µL of streptavidin at 10 µg/mL (VWR) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... to a 50-mL Falcon tube on ice and homogenized at a speed of ∼1000 rpm for 5 sec to brake nuclei clumps with a handheld homogenizer (VWR® 200 Homogenizer). After homogenization ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed overnight at 4 °C with 4% formaldehyde (VWR) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... slides that included the region of interest were washed with PBS before quenching with a solution of 50% methanol (VWR, Radnor, PA, USA) and 3% hydrogen peroxide (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: 250 μL of virus transport medium (VTM) from flocked NP swabs were adjusted to 1.0 mL with 0.1% Triton X-100 (proteomics grade, VWR: 97063-864) diluted in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Approximately 25 mL of the autoclaved media were dispensed into sterile 100 x 20 mm Petri dishes (VWR International, ON) in a laminar flow hood (Design Filtration Microzone).
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bioRxiv - Cancer Biology 2021Quote: ... rinsed beads were dried fully and transferred to a 1.5-mL tube using 100 μL of digestion buffer containing 1.5M urea (VWR 97063-798), 50mM ABC ...
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bioRxiv - Bioengineering 2023Quote: ... Plate colonies were picked at random and used to inoculate 10mL LB media with 100 µg/mL carbenicillin in 15mL culture tubes (VWR). Cultures were once again grown overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were continuously shaken at 220 rpm (100 mL flasks) or 400 rpm (24-well plates) with an orbital shaker (VWR) placed inside the incubator ...
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bioRxiv - Neuroscience 2022Quote: ... Microscope slides with serially cut nerves were rinsed in 1x PBS for 5 min and air dried by incubation at 60°C for 45 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with 90% trypan blue for 5 min and trypan blue exclusion was measured using SD100 counting chambers (VWR; Cat. No. MSPP-CHT4SD100) and a cell counter (Nexcelom Cellometer Auto T4) ...
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bioRxiv - Neuroscience 2024Quote: ... Microscope slides with serially sectioned nerves were rinsed in 1× PBS for 5 min (repeated twice) and air-dried by incubation at 70°C for 10 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Cell Biology 2021Quote: Paraformaldehyde (PFA, 4%; VWR) fixed platelet subpopulations were centrifuged onto poly-l-lysine coverslips (VWR ...