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Citations for VWR :
701 - 750 of 808 citations for 6 Isobutoxy 5 methylpyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Benzonase nuclease was purchased from Sigma (E1014-25KU) and streptavidine sepharose high performance beads (5 mL) was purchased from VWR (GE Healthcare 17-5113-01). OMIX C18 tips (100 µL ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... after which they were permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 minutes and blocked with 5% bovine serum albumin (VWR) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed off the filter with an ice-cold freshly prepared mixture of methanol/ethanol/chloroform (1:3:1) (ethanol LiChroSolv©, Merck, Darmstadt, Germany; chloroform HiPerSolv©, VWR, Dresden, Germany). Extracts were treated in an ultrasonic bath for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... cells were maintained in Joklik’s modified Eagle’s minimal essential medium (US Biological, M3867) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085), 2 mM l-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... concentrated OMVs were diluted 4 times in dH2O and 5 μL of this dilution was added to 150 μL Bradford reagent (VWR). Absorbance was measured at 595 nm and compared to a standard curve to calculate protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... The strains were first passed from agar plates (stored at 4oC) to 5 ml medium (round bottom polystyrene tubes, VWR) until early stationary phase ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 in a 96-well U-bottom plate (Falcon-Corning U-bottom tissue culture plates, VWR, Radnor, PA, USA). In conditions where rhCD80-Fc (Abatacept ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was filtered through a 35µm cell strainer into a 5 ml falcon round bottom tube (VWR #21008-948) for sorting using a MoFlo XDP cell sorter fitted with a 100µm nozzle ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween20) and were fixed in egg buffer with 1% paraformaldehyde for 5 minutes on a Superfrost Plus slide (VWR). Gonads were then flash frozen in liquid N2 and the cover slip was removed ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) and were mounted onto Superfrost Plus microscope slides (VWR) in VECTASHIELD antifade mounting medium (Vector Laboratories) ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Microbiology 2021Quote: L929 cells were grown in either suspension or monolayers in Joklik’s modified Eagle’s minimal essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085). CHO cells were grown in Ham’s F12 medium (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... were washed with 5% HellmanexIII (Lactan, 105513203) in ≥18 MΩ MonoQ H2O at 65°C in a table top Incu-Line oven (VWR) for 4 h and then rinsed 10 times with ≥ 18 MΩ MonoQ H2O ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated for 72 hours at 37°C in 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). All wells had a DMSO concentration of 0.5% v/v with drugs MRX-2843 (25 – 800 nM) ...
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bioRxiv - Microbiology 2022Quote: ... after electrophoresis gels were immersed into a methanol/sodium acetate (pH=5) solution and incubated at room temperature on rocking platform (VWR) at 1 speed for 2 min with constant shaking ...
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bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes prior to incubating for 72 hours at 37°C in a 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). Following incubation ...
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Genomics 2023Quote: ... followed by trituration of brains in 5 ml of DMEM complete media using a glass Pasteur pipette (VWR, 14672-380). The triturated tissue was resuspended of DMEM complete media and filtered through a 40 μm nylon cell strainer (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... purified cells/cell aggregates were washed in PBS and frozen in 5% dimethyl sulphoxide (DMSO; 23486.297, VWR, Radnor, PA, USA) in FBS until xenografting.
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bioRxiv - Bioengineering 2023Quote: ... the 3D printed PCL scaffolds were cut into dimensions of 5 mm × 15 mm using a surgical blade (VWR, Germany) and detached from the glass substrate by 70% EtOH (PanReac AppliChem ITW Reagents ...
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bioRxiv - Plant Biology 2023Quote: ... 4-day old Col_0 seedlings were incubated with LRC liquid medium supplemented with indicated concentrations of compounds (Adenosine 5′-triphosphate magnesium salt, ATP-Mg [VWR] ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... gels were immersed in a methanol-sodium acetate solution (pH 5) and incubated at room temperature on a rocking platform (VWR) at a speed of 1 for 2 min ...
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were then irradiated with UV-A and UV-B for one hour a day for 5 consecutive days [42] in UV crosslinker (cat. no: 89131-486, VWR). On each exposure ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Cell Biology 2024Quote: ... The glands were fixed for 20 minutes at room temperature and then washed in 1x PBS for 3x 5 minutes prior to mounting onto SuperFrost microscope slides (VWR). After removing excess PBS using Whatman paper (Whatman) ...
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bioRxiv - Microbiology 2021Quote: ... and a dye photosensitizer in N,N-dimethylformamide/acetone (7/3, v/v) was electrospun onto one layer of polypropylene (PP) fabrics (VWR® Basic Protection Face Mask). During the electrospinning of 10-20 wt% of PVDF ...
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35150™) was cultured overnight in 3 mL of brain heart infusion (BHI) growth medium (VWR International, LLC, 95021-488) using a shaker-incubator at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting pellets were resuspended in PBS and irradiated at 365 nm for 5 min (100 W lamp, VWR 36595-021) on ice ...
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bioRxiv - Biochemistry 2019Quote: ... pelagicus F5 carbohydrate samples were re-suspended in lyase digestion buffer (50 µL; 25 mM sodium acetate, 5 mM calcium acetate (VWR, UK), pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Immunology 2019Quote: ... mice were reconstituted 4 hrs post-irradiation with 5 million donor bone marrow cells in 200 μl Hank’s Balanced Salt Solution (HBSS; Lonza, distributed by VWR, Lutterworth, UK), administered by intravenous injection into the tail vein ...