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Citations for VWR :
651 - 700 of 910 citations for Methyl 3 2 morpholin 4 ylethoxy benzoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Astrocytes plated at a density of 2 × 105 cells on 12 mm glass coverslips (VWR) coated with 0.1 mg/mL poly-L-lysine (PLL ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2% FBS were injected into the left ventricle of the mice with a 26G × 3/8” needle attached to tuberculin syringe (VWR, Cat: BD309625). During the course of colonization assays ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were then mechanically sleep-deprived 3 sec every min from ZT12-ZT0 using a vortexer (VWR DVX-2500 Multi-tube vortexer). Flies were then taken out of the individual tubes between ZT23 and ZT0 and placed into standard imaging chambers using a mouth pipette ...
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bioRxiv - Microbiology 2020Quote: ... concentrated OMVs were diluted 4 times in dH2O and 5 μL of this dilution was added to 150 μL Bradford reagent (VWR). Absorbance was measured at 595 nm and compared to a standard curve to calculate protein concentration ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Biochemistry 2020Quote: Frozen hippocampus brain tissues were homogenized in a 4 volume of the 10% trichloroacetic acid (VWR International, Radnor, PA, USA) solution using a Kontes glass tissue grinder ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Biophysics 2023Quote: HeLa cells were fixed using 4% paraformaldehyde and permeabilized using Triton-X100 (VWR, Radnor, PA, USA, Cat. No. M143-1L) 12 hours after TTLL6-YFP transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Microbiology 2024Quote: ... then pelleted at 3200 x g for 10 minutes at 4°C and washed twice with pre-chilled blood bank saline (89370-096, VWR). Pellets were resuspended in 0.8 mL 3:1:0.004 acetonitrile:methanol:formic acid + 10 nM verapamil (V105 ...
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bioRxiv - Physiology 2024Quote: ... MPCs were allowed to differentiate for 5 days before adding differentiation medium supplemented with 50 nM 4-OH TAM (89152-604, VWR) for 48 hrs to induce recombination in HSA-miR-1-derived myotubes (or for TAM control in WT-derived myotubes) ...
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bioRxiv - Genomics 2024Quote: ... The suspension containing heads was poured through a 100 µm cell strainer to separate tissue from the collection sucrose buffer and separated into 4 equally aliquots that were each separately homogenized with 7 gentle strokes in a KONTES Tissue Grinder (VWR) (loose pestle ...
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bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 to 30 animals per treatment were incubated at 4°C for 1 hour and then transferred into an electroporation cuvette (Gene Pulser/Micro Pulser Electroporation Cuvette 0.4-cm gap, Bio-Rad or electroporation cuvettes 4-mm gap, VWR) and washed twice with chilled Milli-Q water ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed 4X in Kischkel buffer + 2 μg/ml Aprotinin (VWR, Radnor, Pennsylvania, 97062-752) + 1 μg/ml Pepstatin (VWR 97063-246 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Caco-2 cells were cultured in EMEM (ATCC, 30-2003) with 20% FBS (VWR 89510-186), 1% Pen/Strep (Life Technologies 15140122) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was supplemented with 2 mM calcium chloride and filtered by a glass microfiber filter (VWR). Samples were then loaded by gravity flow onto 5 ml anti-protein C antibody affinity resin ...
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bioRxiv - Microbiology 2020Quote: ... in antibiotic-free LB and 2 mL-deep-96-well plates in checkered pattern (VWR International). Plates were incubated at 37°C with 700 rpm shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL of DMEM infection media containing 0.2% w/v bovine serum albumin (BSA; VWR International) and 1 μg/mL L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 mL of 50 mM ammonium bicarbonate (ABC) + 2 M urea buffer (VWR, M123-1KG). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl cell suspension was loaded under a 22 × 22 mm glass coverslip (VWR, thickness: 1.5). Fluorescence images in Figures 1b ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant was removed and replaced with 500 μL of ACK (ammonium-chloride-potassium) lysis buffer per sample to remove red blood cell contamination (3 min incubation at 37°C; VWR cat#10128-808). Reaction was quenched with 10 mL of cold DMEM/F12-Advanced media supplemented with 10% FBS,1% L-glutamine ...