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Citations for VWR :
651 - 700 of 1623 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Rats were then perfused intracardially with 100ml of 0.9% phosphate saline buffer (PBS) and 100ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Lovaina, Belgium). Then ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted non-sequentially (such that sections on the same slide were at least 4 apart) onto SuperFrost Plus slides (VWR, Lutterworth, UK) and air- dried ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then perfused intracardially with 100 ml of 0.9% phosphate saline buffer (PBS) and 100 ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Louvain, Belgium). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... embedded in paraffin at least 12 hours and sliced (4 μm) using a Leica microtome on Superfrost Plus slides (VWR, 631-0108) prior to drying at 60°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The tumor and all the major organs (heart, lungs, spleen, kidney and liver) were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The animals were sacrificed 72h after the Hypoxisense 680 measurement with Dolethal (Pentobarbital Sodico) (200 mg/ml, Vetoquinol, Aartselaar, Belgium) and the tumors were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA). Only histological procedures were performed on these tumors.
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bioRxiv - Cell Biology 2023Quote: Human AT biopsies were washed twice in PBS to remove excess blood and divided in 50 to 100 mg for fixation in 4% PFA (paraformaldehyde, electron microscopy grade (VWR #100504-858)) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cancer Biology 2024Quote: FFPE tissue blocks containing human primary tumors were sectioned at 4 mm onto Superfrost Plus microscope slides (VWR International, catalog 48311-703). Automated immunohistochemistry was performed at the Oncology Tissue and Imaging Services Core of the Johns Hopkins University School of Medicine using a Ventana Discovery Ultra automated slide staining system (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... overnight growth was prepared by inoculating three colonies into 4 mL GM in round-bottom clear polystyrene culture tubes (VWR, 734-0435) and incubated at 37°C with 200 rpm shaking for 18 hours and 6 days for M ...
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bioRxiv - Bioengineering 2024Quote: Following sacrifice of the animals at day 14 post-injection eyes were enucleated and fixed in 4% stabilized formaldehyde buffer (VWR, Søborg, Denmark) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in a 2 ml lysis buffer using a Dounce tissue grinder (7 ml, VWR). After addition of a 1.8 M sucrose solution (4 mL) ...
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bioRxiv - Neuroscience 2023Quote: ... animals underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a ‘Karlsson-Schultz’ perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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bioRxiv - Bioengineering 2022Quote: ... several milligrams of raw curcumin were dispersed in water and filtered using a 2 μm filter paper (VWR® Grade 410 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of pXLone-dCas9-DNMT3A3L-P2A-EGFP was induced with 2 μg/mL Doxycycline (VWR, Cat# AAJ67043-AD) and GFP-fluorescent cells were enriched by fluorescence-activated cell sorting ...
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bioRxiv - Microbiology 2024Quote: ... duplicate 470 µL aliquots of each culture were removed from the chamber in 2 mL eppendorf tubes (VWR), which were sterilized with SporGon ...
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bioRxiv - Genomics 2022Quote: ... MB135-iDUX4/ZSCAN4-mCherry and MB135-iDUX4 myoblasts were additionally maintained in 2 μg/mL puromycin dihydrochloride (VWR). MB135-iFLAG-SRSF3-FL and -TR myoblasts were additionally maintained in 10 μg/mL blasticidin S HCl (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Cell Biology 2022Quote: ... The remainder of the suspension was transferred to a 2 ml glass Dounce homogeniser (VWR. Cat# 89026-386) and subjected 25 up and down cycles of homogenization ...
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bioRxiv - Bioengineering 2023Quote: ... and sulfuric acid (95-98%; BDH3068-500MLP) and nitric acid (69-70%; BDH3044-500MLPC) were purchased from VWR (PA). The dialysis membrane tubing (MWCO ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were resolubilized with 15 min sonication in 30 μL of 1% acetonitrile + 0.1% trifluoroacetic acid in LC-MS grade water (all from VWR). The injection volume (between 2 and 10 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.1% Fast green (Raymond A Lamb) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min each time, cryopreserved in 30% sucrose (S5-3, Fisher Chemical) in PBS overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (12 μm ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Developmental Biology 2024Quote: ... we generated a sterile circuit by pumping 50 mL of medium from a 50 mL conical tube reservoir using a Masterflex Ismatec Reglo Independent Channel Control Peristaltic Pump (MFLX78001-80, 4-channel, 8-roller; Avantor/VWR, Radnor, PA, USA) fitted with 1.52 mm inner diameter 3-stop tubing (95625-36 ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells transfected with vector-only control or A30P αSyn with and without AcNPs were fixed using a 4% paraformaldehyde (VWR, Cat# ALFAJ61899.AK) in PBS solution for 20 min and washed with PBS and 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cellular debris was then cleared by centrifugation at 1,000 g for 5 minutes at 4°C and then further filtered through a 0.45 μm cellulose acetate filter (VWR cat. no. 28145-481). The cleared media was then concentrated with 100 kDa Amicon cutoff filter (Millipore Sigma cat ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Cell Biology 2021Quote: ... The stable cell lines were plated at 2×106cells/ 35mm cell culture dish (VWR, Radnor, PA, cat # 82050-538). After 24hrs ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were diluted to 2 M urea / 0.1 M NH4HCO3 with 140 μl of HPLC grade water (VWR). Digest with mass-spec grade trypsin (Serva ...
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bioRxiv - Neuroscience 2022Quote: After MRI scanning the shanks were carefully removed and brains were transferred to 2% dimethyl sulfoxide (DMSO, VWR, USA) for cryoprotection for 1-2 days prior to cryosectioning ...
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bioRxiv - Cancer Biology 2020Quote: ... which is prepared by dissolving 2% wt/vol sodium alginate (Protanal LF200S) in water and by adding 0.5mM SDS surfactant (VWR International ...
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Cell Biology 2020Quote: ... Fat droplets were stained through a freshly filtered solution of 0.3 % (w/v) Oil Red O dissolved in 60% (v/v) 2-propanol (VWR). Afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... the canary underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...