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Citations for VWR :
601 - 650 of 914 citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Pellets were then shaken vigorously for 1 min with 2.5–3.5 mm glass beads (332124G; VWR) and bacteria were resuspended in 10 ml macrophage culture media before being centrifuged at 300 × g for 5 min to remove large clumps ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Systems Biology 2021Quote: ... 50 ng lysozyme and PMSF (final concentration of 1 mM) and sonication (Branson Sonifier 450, VWR international). Centrifugation was used to separate soluble proteins from cell debris ...
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bioRxiv - Neuroscience 2021Quote: ... 1:5000 dilution in PBS-T) for one hour and developed with chemiluminescent reaction (VWR, 490005-008).
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bioRxiv - Neuroscience 2021Quote: ... cOFM probes were used and the setup was prepared as follows: 1% Cold Sterilant (VWR #148-0062) was flushed for 30 min and washed out with Ampuwa sterile water (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at a 30-40% confluence on a #1 coverslip (48380-080, VWR, Radnor, PA) placed inside 6-wells (353046 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were subcultured 1:50 (OD 0.05 ± 0.02) in LB medium in 2.2 ml 96-well deep well plates (VWR) and grown to exponential phase (3 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 100,000 cells per well were plated on inserts with 1-μm diameter pores (VWR, cat #62406-173), and the inserts were placed on top of wells with cells plated at 40,000 cells per well density on 24-well culture plates ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
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bioRxiv - Microbiology 2020Quote: ... and wine yeasts (Table 1.) and pre-cultured the products in YPD medium (VWR, Radnor, PA, USA) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 5% CO2 at a density between 0.3-1 x 106cells/mL in RPMI medium (VWR 45000-396) topped up with 10% Fetal Bovine Serum (Genesee Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton X) containing a cocktail of protease and phosphatase inhibitors using a bead disruptor (Omni, VWR). Homogenates were centrifuged at 2100g for 10 min at 4°C and supernatants stored at −80°C ...
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bioRxiv - Bioengineering 2021Quote: ... Left ventricles from donors were sectioned and decellularized using 1% Sodium dodecyl sulfate (SDS, VWR, PA, USA) for 24 h or until the tissues turned transparent white ...
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bioRxiv - Developmental Biology 2021Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) in PBT (1:1000; VWR, 89139-054, Radnor, USA) for 10 minutes and washed with PBT twice for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were harvested and washed with Wash medium (DMEM, 0.1% BSA) + 1 mg/ml DNase (VWR) twice and centrifuged at 300 rcf for three mins ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissociated cells were harvested and washed with Wash media (DMEM, 0.1% BSA) + 1 mg/ml DNase (VWR) twice and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were passaged at 80% confluence and plated on #1 thickness laminin coated coverglass (GG12-laminin, VWR). After allowing cells to adhere to the coverglass for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Organellar pellet was then washed twice and resuspended in 100μL of 1% Triton X-100 (VWR, 0694) in 50mM Tris-HCl and 150mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
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bioRxiv - Cell Biology 2022Quote: ... All cells were finally fixed in PBS containing 1% paraformaldehyde (VWR Life Science brand, Radnor, PA, USA) before data acquisition on either a BD FACS Celesta or a BD FACS Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... prepared as a 1:5 dilution in buffer (pH=7.2) made using Gurr buffered tablets (VWR #331942F) and filtered ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were mounted on micro-scope slides using 4:1 Citifluor:Vectashield solution (VWR and Vector Laboratories) and stored at −20 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
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bioRxiv - Bioengineering 2024Quote: ... The blood placed into a 0.5 ml Greiner Bio-1 minicollection tubes (VWR, International L. L. C.) with ethylene diamine tetra-acetic acid (EDTA ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% fetal bovine serum (Avantor, Radnor Township, PA) and 1% penicillin/streptomycin (VWR, Radnor, PA). Cultures were grown in an incubator set at 37°C with 5% CO2 ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... The pinching oil phase was a heavy mineral oil supplemented with 1% v/v Span-80 (VWR). Downstream of the segmentation region ...
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bioRxiv - Microbiology 2023Quote: ... The homogenate was centrifuged at 13,000 rpm for 1 min (MegaStar® 1.6R, VWR International, Radnor, USA) and the supernatant was transferred to an Eppendorf tube with a 0.8 µm filter and filtered at 13,000 rpm for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... were picked on palladium-copper grids coated with 1% (w/v) formvar (Agar Scientific) in chloroform (VWR). The post-staining was made with 1% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... 1:12 series of sections were quenched for 5 min using 10% H2O2 (VWR, West Sussex, UK) and methanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Spleens were harvested from mice and were placed in 1 mL of RPMI 1640 (VWR International LLC), spleens were then mechanically homogenized using the frosted end of two glass slides (Fisher Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Cell Biology 2023Quote: ... slabs were optically cleared using a 1:2 mixture of benzyl alcohol (Cat. No. 109626; VWR, Sweden) and benzyl benzoate (BABB ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Bioengineering 2024Quote: ... and brain and cardiac organoids were tested in 1×phosphate-buffered saline (PBS) (VWR international, 97063-660). Samples were secured using custom 3D-printed parts specifically designed to fit the sample shape and maintain stability during measurements ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 ml cultures were grown overnight and used to inoculate 1 L of terrific broth media (VWR). Cells were grown until they reached an OD of 0.8 and then cooled on ice before transferring to a 16 °C incubator for overnight induction using 0.5 M IPTG (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... blood was washed out with 2 ml PBS and 1 ml of latex dye (VWR, LL0057-1L) was slowly and steadily injected into the left ventricle with an insulin syringe ...
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bioRxiv - Microbiology 2024Quote: ... The growth experiments were conducted in 1-L DURAN pressure plus bottles (VWR International, LLC, Darmstadt, Germany), which were inoculated with cultures that had been precultured in 50 mL RCM and then in 100 mL PETC medium with fructose ...
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bioRxiv - Immunology 2024Quote: Whole blood was obtained from subjects in cohort 1 and PBMCs were isolated using Ficoll-Paque (VWR) per manufacturer’s instructions and frozen ...
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bioRxiv - Microbiology 2024Quote: ... An individual colony was picked the following day and inoculated into 20 mL of M9ZB media (47.8 mM Na2HPO4, 22 mM KH2PO4, 18.7 mM NH4Cl, 85.6 mM NaCl, 1% Casamino acids (VWR), 0.5% v/v Glycerol ...
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bioRxiv - Biophysics 2024Quote: ... RAW 264.7 and J774A.1 cells were cultured in non-tissue culture-treated 10 cm dishes (VWR); all other cell lines were cultured in tissue culture- treated 10 cm dishes (Corning ...
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bioRxiv - Bioengineering 2024Quote: ... the pH of the solution was adjusted to 7.4 with 1 M sodium bicarbonate (VWR RC-091) and sterile-filtered with a Corning vacuum filter system (0.22 µm ...
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bioRxiv - Cancer Biology 2024Quote: ... and the lung was injected with 1 - 2 mL of 2 % low melting point agarose (VWR, Belgium) in sterile Phosphate Buffered Saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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bioRxiv - Developmental Biology 2022Quote: ... The agarose cubes containing forelimbs were submerged in 1 mL solution containing 0.05% sodium dodecyl sulfate (SDS; VWR), with 2% penicillin-streptomycin or 0.02% sodium azide (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Plant Biology 2020Quote: ... The AIR was digested with 1 mL ultrapure 70% nitric acid (BDH Aristar® Ultra, VWR, Radnor, PA) in 15 mL Teflon beakers ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... through sieves with different mesh sizes (4, 3.15, 2.5, 1.25 and 1 mm, VWR International GmbH, Darmstadt/Germany). Flowthrough of the 1 mm sieve was collected in a pan ...