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Citations for VWR :
601 - 650 of 1403 citations for 6 1 2 4 TRIAZOL 1YL PURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Microbiology 2021Quote: ... ‘Fast’ shaking was established by orbital rotation of flasks at setting 4 on an orbital shaker (Model 3500, VWR, Radnor, PA), which corresponds to ∼200 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... O/N at 4°C before processing for paraffin embedding and the other half was embedded in Optimun Cutting Temperature compound (VWR chemicals). At least 2 serial sections of 5 μm were stained with ...
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bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
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bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... between the starting and ending levels of the removed capillary tubes were measured using a digital caliper (Traceable Carbon Fiber Caliper-4″, VWR International) to determine the volume consumed (after adjusting for evaporative losses) ...
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bioRxiv - Molecular Biology 2024Quote: ... After overnight plating cells were treated with different doses of CDDP for 24 hours or with MMS for 8 hours and fixed with 4% PFA (VWR, AA47377) for 20 minutes and stored in PBS at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... the transgenic gametocytes were pelleted at 1,400 xg for 3 minutes and fixed in 4% v/v paraformaldehyde (VWR, Cat# PI28908) and 0.0075% v/v glutaraldehyde (VWR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then 1 hour in 1:1 ratio of 30% sucrose/0.1M phosphate buffer:OCT (VWR, Cat No. 25608-930). Tissue was embedded inside a mold made by the severed end of a 1.5mL microcentrifuge tube attached to a glass slide with nail polish and stored at −80°C until used ...
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bioRxiv - Genetics 2019Quote: ... All digested products were electrophoresed on 2% agarose gels using Agarose SFR™ (VWR Life Science, USA) for 2 hours at 80V ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Cell Biology 2022Quote: ... Transgene expression was induced with 24-hour incubation in media supplemented with 2 μg/ml doxycycline (VWR). SiRNA-mediated depletion of endogenous ESCO2 was done with 20 nM siRNA (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified cPCR products were examined on 2% agarose gels stained with GelRed (VWR International PBI, Milan, Italy) and visualized on a GelLogic 100 gel documentation system (Kodak ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... spectinomycin (100 µg/ml) and 0.2% arabinose in a 2 ml 96-well deep well block (VWR), sealed with breathable film (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... Cells used for in vitro electrophysiology experiments were grown on glass coverslips (12×12mm, No.2, VWR). Patch-clamp experiments were performed in aCSF consisting of (in mM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 x 107 cells per replicate were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... then cells were resuspended in sterile PBS with 2% FBS and filtered (70 μm; VWR, #10204-924).
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bioRxiv - Cell Biology 2023Quote: ... 2 µL cell suspension was loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5).
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Molecular Biology 2021Quote: ... and G418 sulfate (500 μg mL−1, from 50 mg mL−1 stock in H2O, from 712 U mg−1 powder, VWR). Cells were checked monthly for mycoplasma contamination by PCR assay ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... 1% (w/w) 10:1 (base/crosslinker w/w) Sylgard-184 (VWR) was added to the GEL-8100 and mixed well for 60 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample was homogenised in 1 mol.L-1 HCl (VWR, United States). Free glucose content was measured at this stage ...
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bioRxiv - Immunology 2023Quote: ... HOIP (VWR/Proteintech 16298-1-AP, SHARPIN (VWR/Proteintech 14626-1-AP), and actin (Sigma-Aldrich A5316 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was homogenized in 1 mol.L-1 HCl (VWR, United States) and free glucose content was measured ...
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bioRxiv - Genetics 2021Quote: ... 1 mM Squalene (VWR), 50 µM Lanosterol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% BSA (421501J, VWR), 0.1% Triton-X and 0.05% Tween-20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2 (VWR), 1% Triton X-100 (Alfa Aesar) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% penicillin-streptomycin (VWR, 5 units/mL penicillin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... 1 cover glass (VWR) was used for the top plate and a 40 mm cover glass (Fisherbrand ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: The four main arteries of PFA-fixed intact human brain were connected to a peristaltic pump (ISMATEC, REGLO Digital MS-4/8 ISM 834) through a chemical-resistant PTFE tubing (VWR, 228-0735) in a glass chamber ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were then perfused intracardially with 100ml of 0.9% phosphate saline buffer (PBS) and 100ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Lovaina, Belgium). Then ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then perfused intracardially with 100 ml of 0.9% phosphate saline buffer (PBS) and 100 ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Louvain, Belgium). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...