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Citations for VWR :
601 - 650 of 823 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Trifluoroacetic acid, acetonitrile and 2-propanol were from Biosolve BV (Valkenswaard, The Netherlands) and glycine was from VWR International (Radnor ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in a 2 ml lysis buffer using a Dounce tissue grinder (7 ml, VWR). After addition of a 1.8 M sucrose solution (4 mL) ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... animals underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a ‘Karlsson-Schultz’ perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
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bioRxiv - Bioengineering 2022Quote: ... several milligrams of raw curcumin were dispersed in water and filtered using a 2 μm filter paper (VWR® Grade 410 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of pXLone-dCas9-DNMT3A3L-P2A-EGFP was induced with 2 μg/mL Doxycycline (VWR, Cat# AAJ67043-AD) and GFP-fluorescent cells were enriched by fluorescence-activated cell sorting ...
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bioRxiv - Microbiology 2024Quote: ... duplicate 470 µL aliquots of each culture were removed from the chamber in 2 mL eppendorf tubes (VWR), which were sterilized with SporGon ...
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bioRxiv - Genomics 2022Quote: ... MB135-iDUX4/ZSCAN4-mCherry and MB135-iDUX4 myoblasts were additionally maintained in 2 μg/mL puromycin dihydrochloride (VWR). MB135-iFLAG-SRSF3-FL and -TR myoblasts were additionally maintained in 10 μg/mL blasticidin S HCl (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The remainder of the suspension was transferred to a 2 ml glass Dounce homogeniser (VWR. Cat# 89026-386) and subjected 25 up and down cycles of homogenization ...
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bioRxiv - Microbiology 2024Quote: ... RLMVECs assayed for Mx1/2/3 expression during SEOV infection were fixed using 4% paraformaldehyde (VWR, 97064-606) solution in sterile PBS for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: A bubbling system was set up with 2 flasks of 1 M NaOH (VWR, catalog no. 97064-476) DI H2O (PURELAB Flex ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting pellets were resuspended in PBS and irradiated at 365 nm for 5 min (100 W lamp, VWR 36595-021) on ice ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
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bioRxiv - Microbiology 2020Quote: ... Cells were covered with Eagle’s Minimum Essential Medium (without phenol red, supplemented with 5% FBE, non-essential amino acids, 1mM sodium pyruvate (VWR, 45000-710), 2mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... one holes were bored into the lid of a Drosophila bottle and inserted a 5-μl glass capillaries (VWR, 53432-604) which filled with 20% sucrose ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and C were filled with 25 µl of 40 µM oligomycin for a final in-well concentration of 5 µM (“Oligo,” VWR Scientific), 4.5 µM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone for a final in-well concentration 0.5µM (“FCCP,” VWR Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... we added another 0.5 ml PBS to the tubes that later were sonicated in the water bath of an ultrasonic cleaner (USC200TH, VWR International) for 5 min at 45 kHz ...
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bioRxiv - Cancer Biology 2024Quote: A total of 5 × 106 MM.1Sgfp+/luc+ cells were seeded in T-25 flasks (Avantor/VWR, Cat. No. 10861-568) and treated with the vehicle (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... The 18 cryovials were tightly capped and placed in a 5 cm square cardboard cryobox that was immediately placed in a LN dewar (VWR CryoPro).
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bioRxiv - Immunology 2023Quote: ... brains were isolated and placed into a 50 mL conical tube (Fisher Healthcare) containing 5 mL of RPMI (VWR International LLC). The RPMI containing the brains of the mice were then transferred to a 7 mL glass Tenbroeck dounce homogenizer (Pyrex ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Immunology 2024Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed three times with 1X PBS (5 minutes each) and mounted on SuperFrost Plus micro slides (VWR 48311-703). Slices were covered with a 22 mm x 50 mm 1.5 thickness coverslip using ProLong Glass Antifade Mountant media (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... A 5 mg aliquot of each sample was subjected to extraction using methanol (HPLC - gradient grade, VWR International LLC, Randor, USA). The extraction process involved homogenization with 10 ceramic balls at a velocity of 6.5 m/s for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The stable cell lines were plated at 2×106cells/ 35mm cell culture dish (VWR, Radnor, PA, cat # 82050-538). After 24hrs ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were diluted to 2 M urea / 0.1 M NH4HCO3 with 140 μl of HPLC grade water (VWR). Digest with mass-spec grade trypsin (Serva ...
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bioRxiv - Neuroscience 2022Quote: After MRI scanning the shanks were carefully removed and brains were transferred to 2% dimethyl sulfoxide (DMSO, VWR, USA) for cryoprotection for 1-2 days prior to cryosectioning ...
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bioRxiv - Cancer Biology 2020Quote: ... which is prepared by dissolving 2% wt/vol sodium alginate (Protanal LF200S) in water and by adding 0.5mM SDS surfactant (VWR International ...
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bioRxiv - Cell Biology 2020Quote: ... Fat droplets were stained through a freshly filtered solution of 0.3 % (w/v) Oil Red O dissolved in 60% (v/v) 2-propanol (VWR). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... the canary underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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bioRxiv - Microbiology 2023Quote: ... Inoculums of 100 µL were delivered by intra-gastric gavage using a 2-inch-long plastic feeding needle (VWR) connected to a 1 mL syringe (Becton Dickinson) ...
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bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).