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Citations for VWR :
6401 - 6450 of 8568 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... equal amounts of the fibrillated sample were aliquoted into 8 tubes and the fibrils were pelleted by centrifugation (13,500 rpm, 15 min) in a MicroStar12 table centrifuge (VWR) and the supernatants were removed and the concentration of un-fibrillated monomer was measured to determine the exact amount of fibrillated protein ...
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bioRxiv - Biophysics 2020Quote: ... Sodium chloride 99% pure obtained from VWR Scientific ...
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bioRxiv - Biophysics 2020Quote: ... was first dissolved in Dimethyl Sulfoxide (DMSO, VWRVN182, VWR) at 5 mg/ml and aliquoted into 5 μl for each vial and stored in −80 °C ...
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bioRxiv - Cell Biology 2020Quote: ... N2a cells were transfected using jetPEI reagent (Polyplus transfection/VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Stable cell lines were generated as follows: N2a cells were transfected with PANX1-EGFP using the jetPEI reagent (Polyplus transfection/VWR) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... Confocal Microscopy—HeLa cells grown in 35 mm imaging dishes (made in-house from Corning 35 x 10 mm dishes with VWR 18 x 18 mm #1.5 cover slips) were imaged 48 hours post transfection to maintain consistency with the FACS measurements ...
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bioRxiv - Biophysics 2020Quote: ... U2OS cells were grown in 35 mm imaging dishes (made in-house from Corning 35 x 10 mm dishes with VWR 18 x 18 mm #1.5 cover slips). All CyTERM constructs were transiently transfected for 18–24 hours using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Cells or lung tissue were lysed in RIPA buffer (VWR) supplemented with protease (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2020Quote: ... Ascorbic acid was purchased from VWR. MitoSOX ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: Wilt-type and ISL2 KO cells were plated in micro cover glasses (VWR cat. no. 48368-062) at a density of 2×104 in regular growth conditions ...
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bioRxiv - Molecular Biology 2020Quote: Sterile empty 100 × 15 mm Petri plates (VWR International ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted using RIBOZOL™ (VWR, Pennsylvania, U.S.). For larvae ...
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bioRxiv - Cell Biology 2020Quote: ... ovaries were dissected and imaged in 10S Voltalef oil (VWR Chemicals). Images were processed with Fiji (Schindelin et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal bovine serum (Seradigm 1500-500; VWR International, West Chester, PA, USA), penicillin and streptomycin (15140 ...
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bioRxiv - Cell Biology 2020Quote: Confluent monolayers of h-iECs in a 100-mm culture dish were subjected to orbital shear stress for 24 h at a rotating frequency of 150 rpm using an orbital shaker (VWR, Model 1000) positioned inside a cell culture incubator ...
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bioRxiv - Cell Biology 2020Quote: ... fixed in 4% neutral buffered formaldehyde (VWR, Darmstadt, Germany), cell number was determined by DAPI staining (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... After 10 min of fixation using 4% neutral buffered formaldeyde (VWR, Darmstadt, Germany), the cell count was assessed by DAPI-staining (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and 500 µl of ethyl acetate (ethyl acetate; VWR) following which they were centrifuged at 500g at RT for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... 60 μl of 4M perchloric acid (VWR) were combined and vortexed ...
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bioRxiv - Cell Biology 2020Quote: ... 120 µl of 6M perchloric acid (VWR) were combined and homogenized with a tissue homogenizer ...
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bioRxiv - Cell Biology 2020Quote: ... The derivatized extract was centrifuged at 500 g for 15 min and the supernatant transferred to a tube containing 0.08 g sodium sulfate (VWR) and 500 µl of ethyl acetate (ethyl acetate ...
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bioRxiv - Cell Biology 2020Quote: 150 µL of extract and 500 µL of 25 mM OPD in 2M HCl (made from o-Phenylenediamine, 98%; VWR) were combined ...
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bioRxiv - Cell Biology 2020Quote: ... This step was repeated twice and the supernatant collected were vacuum centrifuged at 30°C for 45 min Samples were then reconstituted in 64 µL of 200 mM ammonium acetate (made from; ammonium Acetate, 98%; VWR) and transferred to amber glass UPLC vials (Waters) ...
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bioRxiv - Cell Biology 2020Quote: ... plunger (89026-398) and glass vessel (89026-386) for homogenizing cells from VWR; Odyssey Buffer from Licor (927-40000) ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... We then added β-estradiol (from VWR, #AAAL03801-03) to a final concentration of 100 nM and shook the cultures at 32°C for four hours ...
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bioRxiv - Cell Biology 2020Quote: ... Cryosections were cut at a thickness of 10-16 µm using a Leica CM3050 S cryostat and placed onto SuperFrost®Plus™ slides (VWR). Prior to staining ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... RPE-1 cells were incubated in medium containing 356 µM cycloheximide (VWR Chemicals, 441892A) for 6h.
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked by 5% Powdered milk (Roth, T145.3) in PBS-0.2% Tween20 (VWR Life Science, M147-1L), detected with the indicated antibodies and analyzed by quantitative near-infrared scanning (Odyssey ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% propionic acid (VWR), 0.1% Nipagin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The cell solution was passed through a 70 µm strainer (VWR) once to filter out any cell clumps ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed with 30 passes through a tissue homogenizer (VWR Cat. No. 89026-386), followed by pelleting nuclei and un-lysed cells for 3 min at 3,000×g to obtain a post-nuclear supernatant (PNS) ...
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bioRxiv - Cell Biology 2020Quote: ... dechorionated embryos were mounted on glue-coated coverslips (0.17mm, grade #1.5) and covered with Voltalef 10S oil (VWR). Movies of GFP::M6 accumulation were acquired using a 40x/1.3 NA oil immersion objective and the SP8 resonant scanner (line accumulation of 2 ...
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bioRxiv - Cell Biology 2020Quote: ... were diluted 1:50 in phenol red-free matrigel (Corning; VWR) and added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Cell Biology 2020Quote: ... penicillin and streptomycin (1406-05-9; VWR International). African Green Monkey kidney epithelial MA104 cells (a kind gift from Siyuan Ding ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow was collected via centrifugation from the femur and tibia of 8-week-old male C57BL6/J mice and cultured in αMEM (VWR, Radnor, PA, USA) supplemented with 10% fetal bovine serum VWR ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal bovine serum VWR) and 1% Pen-Strep (VWR). After 48 hr ...
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bioRxiv - Cell Biology 2020Quote: Dechorionated embryos were immobilised with glue on a coverslip and covered with Oil 10-S Voltalef (VWR). To visualise tracheal Shot in vivo ...
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bioRxiv - Cell Biology 2020Quote: Gastrocnemii were homogenized with 25 strokes using a 5 mL PTFE tissue grinder with clearance 0.15-0.25 mm (VWR 89026-392, 89026-404) at 3,000 rpm in RIPA lysis buffer (VWR 97063-270 ...
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bioRxiv - Cell Biology 2020Quote: ... The injection was performed with a Hamilton syringe (VWR 72310-316) and the injection volume of the cell suspension was 10 μL ...
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bioRxiv - Cell Biology 2020Quote: ... at 3,000 rpm in RIPA lysis buffer (VWR 97063-270) supplemented with Roche cOmplete Mini Protease Inhibitors (Roche 04693124001 ...
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bioRxiv - Cell Biology 2020Quote: ... The precipitate was resuspended in 200 µl extraction buffer (150 mM Tris-HCl, pH 6,8 (#443866G, VWR, USA); 6% SDS (#CN30.3 ...
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bioRxiv - Cell Biology 2020Quote: ... Art2-HTF and Pgk1 or to nitrocellulose membranes (#10600004, VWR, USA) for the detection of ubiquitinated Mup1 ...
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bioRxiv - Cell Biology 2020Quote: ... Starvation medium contained 1,7g/L Yeast Nitrogen Base without amino acids and ammonium sulfate (#J630-100G, VWR, USA) and 2% glucose (#X997.5 ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from total cells and eluates from immuno-precipitations were separated by standard SDS-PAGE and transferred to PVDF membranes (#10600023, VWR, USA) for the detection of Mup1-GFP ...