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Citations for VWR :
551 - 600 of 964 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized by treatment with 250 µL PBS + 1 mM CaCl2 (0556, VWR), 1 mM MgCl2 (M8266 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was resuspended in 25mL ice-cold 10% glycerol (VWR, #EM-4750). These centrifuge-resuspension steps were repeated for two more times with ice-cold 10% glycerol ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed in cold 0.5% Dodecyl-β-D-maltopyranoside (DDM, VWR-97063-172) lysis buffer (20 mM Tris-Cl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with ice cold 0.9% NaCl (VWR BDH Analytical #BDH9286-2.5KG), then 1mL of ice cold 50% MeOH [HPLC-grade (Sigma-Aldrich #A456-4) ...
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bioRxiv - Molecular Biology 2023Quote: 10,000 cells/well were seeded in a 96-well glass bottom plate (VWR, GREI655891_16). 24 hours after seeding ...
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bioRxiv - Immunology 2023Quote: ... After lysis of red blood cells with ACK lysis buffer (VWR; Radnor, PA, USA) cells were filtered through a 40 μM cell strainer and grown on petri plates in DMEM containing 10% FBS and 30% L929 conditioned media for 7 days with fresh L929 containing media media being added on day 4 post plating ...
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bioRxiv - Cell Biology 2023Quote: ... 9,000 cells were plated into a low attachment U-bottomed 96 well plate(VWR) in hESC media supplemented with 50µM Y-27632(LC Labs ...
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bioRxiv - Biophysics 2023Quote: ... Nematodes homogenization and cell disruption were performed by using an ultrasonic bath (VWR, USC300TH) four times ...
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bioRxiv - Microbiology 2023Quote: UppSVU and UppSUN were purified from BL21 ArcticExpress DE3 RIL cells (VWR, Radnor, PA). Cells expressing pET-15b-UppSVU or pET-15b-UppSUN were grown in 10 mL LB containing carbenicillin overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and cells on coverslips were fixed with 10% Neutral Buffered formalin (VWR, 16004-128) for 1hr ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were detached for passaging by 5 min incubation with trypsin-EDTA (0.25%, VWR). MDA-MB-231 cells were used in studies between passages 45 and 50 ...
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bioRxiv - Microbiology 2024Quote: ... or cells smears were prepared on Superfrost micro glass slides (Cat#48311-703, VWR) as described previously [77] ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellets were resuspended in 1X PBS containing 1% formaldehyde (AA33314-K2, VWR) and protease inhibitors ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell quantity and viability were determined via staining by Trypan Blue (VWR, Cat. #VWRVK940). Sortilin KO cells were generated using a Lonza 4D Nucleofector X nucleofector and a Synthego v2 gene KO kit with SORT1 sgRNA (custom-ordered ...
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bioRxiv - Molecular Biology 2024Quote: MEFs were grown to 80–90% confluency in standard 10cm cell culture dish (VWR) and cultured in complete medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates were obtained via mechanical lysis using a needle (VWR-TERUMO, AN2138R1) and RIPA cell lysis buffer (Sigma/Merck ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were cultured on poly-L-lysine-coated 18 mm glass coverslips (VWR) at 60% - 70% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... and Jurkat cells were adhered on polysine slides for 15 min (VWR, 631-0107). For ciliogenesis and centriolar satellite staining ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using the Qiagen QuantiTect Reverse Transcription Kit and analyzed using the PerfeCTa SYBR green FastMix (VWR) and a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Genomics 2020Quote: Library preparation for each CUT&RUN antibody condition was done with a KAPA Hyper Prep Kit (VWR, 89125-040) with the PCR amplification settings adjusted to have simultaneous annealing and extension steps at 60 °C for 10 seconds ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and cDNA was synthesized with the peqGOLD MicroSpin Total RNA Kit (Peqlab/VWR Life Science, 12-6831) and the qScript™ Flex cDNA Kit (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Molecular Biology 2021Quote: ... 1ug of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 1µg of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Tissues were homogenized with a Minilys homogenizer (Bertin Technologies, Rockville, MD) using a Precellys Lysing Kit (VWR Scientific, Radnor, PA). Total RNA was extracted from thoroughly homogenized tissues via miRNeasy Mini Kit (#217004 ...
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bioRxiv - Immunology 2020Quote: ... Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069 ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were re-suspended in 500 μl of 5 mg/ml lysozyme (VWR) and incubated for 20 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of cells were transferred to 4.5 mL disposable cuvettes (VWR Cat# 30620-276). Ethidium bromide (6.25 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: Astrocytes were seeded at a density of 1x105 cells on 12 mm glass coverslips (VWR) coated with poly-L-lysine ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were seeded at a density of 4x105 cells in 24 mm glass coverslips (VWR) coated with poly-L-lysine ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed in phosphate-buffered saline (PBS) and stained with 0.1% Crystal Violet (VWR) in 20% methanol ...
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bioRxiv - Genomics 2020Quote: ... two million cells were resuspended in 100 μl cold BTXpress electroporation solution (VWR 732-1285) with 20 μg Cas9 ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell suspension is passed through 40um pore size strainer (Falcon®, VWR 734-0004), and the strainer is washed by adding 2ml of accutase on the strainer ...
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bioRxiv - Neuroscience 2019Quote: ... The cell suspension was subsequently lysed using a probe sonicator (Branson Sonifier 250, VWR Scientific) on ice ...
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bioRxiv - Biochemistry 2021Quote: ... hippocampal neurons (85000 cells/ml) were plated on poly-D-lysine-coated glass coverslips (VWR) in 12-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... sections were transferred to 0.4 μm polycarbonate membranes cell culture inserts (VWR, Radnor, PA, USA) and incubated in Claycomb medium supplemented with 10 % foetal calf serum ...
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bioRxiv - Plant Biology 2020Quote: ... Spores were rinsed from their storage buffer using a 40 µm nylon cell strainer (VWR) by five washes with sterile UHQ water ...
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bioRxiv - Microbiology 2021Quote: ... cells were immobilised under agarose pads prepared using microarray slides (VWR, catalogue number 732-4826) as described in de Jong et al. ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells in the spleen and thymus were lysed with ACK Lysing Buffer (VWR) or RBC Lysis Buffer (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... Cells were grown in 600 μL of medium in 96-well deep well plates (VWR). Plates were sealed with a gas-permeable microplate adhesive film (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in PBS and were mounted on VistaVision glass microscope slides (VWR) using Prolong Gold antifade reagent (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... 5 × 105 cells were cytospun onto 22 × 60 mm #1.5 coverslips (VWR international, Batavia, IL), pre-coated with CellTak (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...