Labshake search
Citations for VWR :
551 - 600 of 1419 citations for 4 1 tert butoxycarbonyl piperidin 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
-
bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
-
bioRxiv - Molecular Biology 2024Quote: Purified chromosomes 3 or 19 were cytocentrifuged (Cytospin3, Shandon) at 163 g for 10 min onto Polysine slides (VWR). After mounting with Vectashield containing DAPI (Vector Laboratories) ...
-
bioRxiv - Biophysics 2022Quote: ... Cell aggregates on top of the droplets were transferred to an electroporation cuvette (4 mm gap, VWR). Three square wave pulses of 1 kV for 70 μs were applied with 1 s intervals between each pulse using an ECM 2001 Electro Cell Manipulator (Harvard Apparatus ...
-
bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2021Quote: ... an aqueous solution of sodium dodecyl sulfate (SDS; concentrations: 2% or 4%; Cat. No. 97064-472, VWR) was instilled directly into the trachea via an inlet cannula ...
-
bioRxiv - Immunology 2021Quote: ... cells were resuspended in cold PBS/0.04% BSA and fixed by adding 4 volumes 100% Methanol (VWR) and incubation for 30 min at -20 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Organoids were fixed in 4% PFA and embedded in Tissue-Tek® O.C.T (VWR, cat # 25608-930). Slides of cryosectioned organoids were blocked in 5% normal donkey serum (Jackson Immuno Research ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... VWR Spinbar Micro Stir Bars and 4-ml Amber Borosilicate Glass sample vials were purchased from VWR.
-
bioRxiv - Cancer Biology 2023Quote: ... and 0.5% Triton X-100) on ice for 2 min and immediately fixed in formaldehyde 4% (VWR) for 10 min at the room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... 1e8 bacteria from each strain were pelleted and resuspended in 500 μL 4% paraformaldehyde (VWR, AAJ61899-AP) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... they were transcardially perfused with cold (4 °C) sterile saline and then 10% formalin (VWR, Radnor, PA). The brains of the mice were extracted from the skull ...
-
bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
-
bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
-
bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
-
bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
-
bioRxiv - Bioengineering 2021Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
-
bioRxiv - Microbiology 2020Quote: ... and 3 μL of the culture was inoculated into 3 mL of MSgg liquid media in a 6-well or 12-well microtiter plate (VWR). The plates were incubated at 30°C for 2-3 days ...
-
bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were washed with PBS 3 times and fixed and permeabilized with 3.7% formaldehyde and 0.3% Triton-X (VWR 80503-490) in PBS for 30 minutes at room temperature ...
-
bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
-
bioRxiv - Microbiology 2021Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
-
bioRxiv - Genetics 2022Quote: ... and an equal volume of 1x Sperm Salts with 3% paraformaldehyde was applied for 5 min before samples were affixed to a Superfrost Plus slide (VWR). For both protocols ...
-
bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
-
bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
-
bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
-
bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
-
bioRxiv - Molecular Biology 2023Quote: Monocytes were isolated as described above and 3×106 cells/ml were plated in a 6-well plate using RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
-
bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
-
bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
-
bioRxiv - Bioengineering 2022Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
-
bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
-
bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... At least 3 colonies per strain were picked and inoculated in 2 mL-deep 96 well plates (VWR 76397-576) with 1 mL of MPG supplemented with 50 μg/mL ampicillin and 100 μL of 20% dextrose ...
-
bioRxiv - Cancer Biology 2024Quote: ... Excess DAPI was cleared with 3×5 min washes in PBS and slices were then mounted on glass slides (VWR) using a ProLong Gold antifade reagent (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... which we then incubated at 25°C for 4 days prior to staining with iodine (VWR iodine crystals) vapor (Forsburg and Rhind 2006).
-
bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was kept overnight in a solution of PBS 0.01 mM/formaldehyde 4% (v/v; CAT#20909.330, VWR) and then cut in 50 μm slices with a vibratome (VT1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Mice were then perfused with 30 mL of cold PBS and 4% paraformaldehyde (#100503-916, VWR, Radnor, PA) and were immediately decapitated to collect the brains ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fetuses and brains were then stored long-term at 4°C in 0.002% Sodium Azide (VWR - Bie&Berntsen) in PBS.
-
bioRxiv - Physiology 2023Quote: ... a reversed-Phase C18 LiChroCART 250-4 column (LiChrospher 100 RP-18, 5 μm, VWR, Radnor, PA, USA) was used ...
-
The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were mounted on ∼1.4% w/v agar pads (in sterile ultrapure water) and a 0.13- to 0.17-mm glass coverslip (VWR) was placed on top ...
-
bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were fixed with 4% PFA for 15 min and incubated with 40 mM Alizarin Red S (VWR, US) for 30 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... reference slides were generated using 4-micron thick sections mounted on SuperFrost Plus slides (VWR International, Lutterworth, UK) and reviewed by the neuropathologist who performed the autopsy ...