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Citations for VWR :
451 - 500 of 548 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting material was well resuspended with keratinocyte media and then filtered through a 70 μm cell strainer (VWR, Corning). The cell suspension was then centrifuged for 7 min at 4 °C 1500 rpm and washed twice with PBS ...
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bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 in a 96-well U-bottom plate (Falcon-Corning U-bottom tissue culture plates, VWR, Radnor, PA, USA). In conditions where rhCD80-Fc (Abatacept ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Biochemistry 2022Quote: ... The 500 mL culture (OD550 ∼0.6) was then transferred into the carboy via sterile rubber transfer line (VWR-62993-726), 18-gauge needles (Becton Dickinson 305196 ...
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bioRxiv - Microbiology 2020Quote: ... thetaiotaomicron 7330 tdk-strain was generated by UV mutagenesis by exposing a liquid culture of 7330 to 320 nm ultraviolet light from a VWR-20E transilluminator (VWR) for 60 seconds and plating onto BHIS-Blood agar supplemented with 200 micrograms/mL of 5-fluoro-2’-deoxyuridine (FUdR) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Neuroscience 2020Quote: ... penicillin/streptomycin and amphotericin B (1X Anti/Anti, #15240062, TFS) (referred to as ‘SHM-10’) on uncoated cell culture flasks (VWR). Cultures were maintained in a sterile incubator (MCO-20AIC ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... 200 µl of this resuspended culture was aliquoted into a 96-well black/clear glass bottom sensoplate (Greiner Bio-one VWR). Four technical replicas were measured per culture ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Biophysics 2020Quote: ... The bottom part of the PMMA chamber contains the ellipsoid culture wells (diameters: 3.5 mm, 6.5 mm) and is glued onto a microscopy cover glass (VWR, Radnor, USA) using PDMS (Sylgard® 170 silicone ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Cell Biology 2023Quote: ... Germany) using standard protocols and transferred to glass-bottom cell culture chambers (cat#631-0150, VWR International, Radnor, PA, USA). 24 h post gene silencing ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... supplemented with PIM(ABS) (Prodo Labs) and PIM(G) (Prodo Labs) and incubated in a T-150 non-tissue culture treated flask (VWR) for recovery at 37°C and 5% CO2 overnight ...
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bioRxiv - Microbiology 2024Quote: ... Liquid bacterial cultures were grown to mid-log phase in 100 μL per well in a round-bottomed 96-well plate (VWR), centrifuged (5,000g ...
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bioRxiv - Microbiology 2023Quote: Once primary airway epithelial cells reached ∼80% confluence, they were cultured on collagen coated cell culture inserts (Transwell®, 24-Well, 0.4 μm PET clear) (VWR) as previously published (Moreni et al. ...
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µL of each was added to 2 mL cultures of attached cells in 6-well plates (#10861-554, VWR) containing 0.2 mM CaCl2 ...
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bioRxiv - Biophysics 2022Quote: Muscle tissue culture molds were fabricated from polymethyl methacrylate (PMMA) and glued onto a microscopy cover glass (VWR, Radnor, USA) as previously described[22] ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 ml MethoCult mixed with the cell suspension was slowly taken up using a pipette and then transferred to a 35 mm cell culture dish (VWR). The cell culture dish was cautiously tilted until it was covered with medium and was put inside the incubator ...
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bioRxiv - Microbiology 2023Quote: ... were generated by centrifugation of the grown culture at 5800 x g for 20 min and subsequent filter-sterilization (0.2 µm, VWR™). SM samples were prepared freshly before each experiment under anaerobic conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... measurement of 0.01 in 200 uL growth medium per well in a transparent 96-well plate with a flat bottom (FalconR 96-Well Cell Culture Plates, Corning, 15705-066, VWR) with appropriate antibiotics and inducers ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (VWR, 392-0413) supplemented with 10 % fetal bovine serum (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Live cells in culture were imaged using a MoticamPro 205A camera mounted on a Trinocular Inverted Microscope (VWR 89404-462).
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bioRxiv - Neuroscience 2024Quote: ... a 1-3 day old virgin female was placed in a 5.0 ml culture tube (VWR, Cat. No. 60818-565) in an ice bath for approximately 30 seconds until the fly was completely immobilized ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: One million viable cells (≥ 10 µm diameter) were plated (N0) into uncoated 3.5 cm Falcon™ plastic tissue culture dishes (Corning/VWR) containing 2 mL arginine-free Dulbecco and Vogt’s Modified Eagle’s Medium (standard plating conditions) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed tissue was placed in sucrose solution (30% sucrose in PBS) for 24h and frozen in tissue mounting medium (OCT mounting media, VWR chemicals). The tissue was coronally sectioned at 50-60µm thickness on a cryostat ...
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bioRxiv - Bioengineering 2020Quote: ... biofilm from the wet-end (BF) and the effluent from the biological treatment plant (BTP) were individually microbiologically enriched in M9 media (VWR, USA) (Miller ...
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bioRxiv - Immunology 2020Quote: ... the monocytes were harvested and the pellet resuspended in differentiation media (RPMI-1640 supplemented with 1% FBS H.I., Penicillin/Streptomycin (P/S, VWR, Radnor, PA) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant media containing viral particles was harvested and filtered through a 0.45 µm polyethersulfone (PES) filter (VWR, Randor, Pennsylvania, US) and aliquoted ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- + bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- +bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media with and without thiamine in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Immunology 2023Quote: ... Plaque assays were developed at 5dpi by removal of overlay media and staining/fixation using 10% neutral buffered formalin (VWR, #89370) and 0.25% crystal violet (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in embryo media as a vehicle (E3; see section 2.3) and were maintained in a non-CO2 incubator (VWR, Mississauga, Ontario) at 28°C on a 14h:10h light-dark cycle ...
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bioRxiv - Microbiology 2024Quote: ... Pads were then washed with pre warmed media before application of the cover slip (VWR 22 × 22 mm2 Thickness no. 1.5).
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 200 µl of mounting media (VectorLabs, H10000-10) was applied directly onto the sections before covering with coverslips (VWR, 48393- 251). The slide-mounted sections were scanned using a confocal microscope (Nikon A1R HD25).
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bioRxiv - Immunology 2020Quote: ... equal numbers of cells were counted by Moxi Mini automated cell counter (Orflo) and 0.6×10^6 cells per data point were seeded in T-25 cell culture flasks (VWR, PA, USA) in appropriate volume of culture medium ...
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bioRxiv - Developmental Biology 2023Quote: ... 4.5 μM or 6.0 μM in KR5 or hBI medium under shaking culture at 120 rpm (VWR Orbital Shaker Model 1000) for 2 days (D0-D2) ...
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were plated in 24 well CELLSTAR® cell culture plates (for bulk-RNA-seq. and live imaging; VWR, 82050-892), µ-Slide 8 Well (for Video S1. ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...