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Citations for VWR :
451 - 499 of 499 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 2 ml MS grade water and eicosanoids were eluted with 500 µL methanol (MeOH abs.; VWR International, Vienna, Austria) containing 2% formic acid (FA ...
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bioRxiv - Biophysics 2022Quote: ... Cells were trypsinized and seeded on the substrate at a density of 30,000 cells cm-2 in a glass-bottom microscopy dish (VWR, 734-2906H, Barcelona, Spain) in chondrogenesis-inducing medium (ATCC ...
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bioRxiv - Bioengineering 2024Quote: ... All dilutions of targets were performed in 4× PBS with 2 μM dT10 in GeneMate low-adhesion 1.7-mL microcentrifuge tubes (VWR, Cat No. 490003-230). PCR tubes containing target mixtures were then heated in a thermocycler to 73 °C for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... the substrates and products were visualized under a UV lamp at 254 nm and placed separately in vials in 2 ml of Scintran (VWR, Radnor, PA, USA). The radioactivity in disintegrations per minute (dpm ...
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bioRxiv - Microbiology 2024Quote: ... a multi-element calibration standard containing each element was prepared using 1000 mg /L single-element standards (SPE Science, Canada) diluted with 2% (v/v) HNO3 (Aristar grade, VWR International, United Kingdom). The limits of detection for each element in He mode were 0.005 ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Biophysics 2022Quote: Before MS analysis, peptides were resuspended in loading buffer (2% ACN, 0.1% FA) and completely solubilized in a bath sonicator (VWR, 35 kHz, 10 min).
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bioRxiv - Microbiology 2024Quote: ... each mat core was submerged in 2 ml of sterile PBS 4X and mechanically macerated with sterile polypropylene pestles and an electrical homogenizer (VWR, Wayne, PA, USA). The organic phase was recovered and centrifuged per 5 min to 10,000 rpm ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Physiology 2020Quote: ... with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals, Leicestershire, UK) and 0.7 ml of Tris (1M ...
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bioRxiv - Microbiology 2022Quote: ... Biofilms were incubated with anti-Candida antibodies diluted into dilution buffer (PBS with 0.05% Tween (VWR M147-1L), 0.1% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... primary antibodies were added to the blocking solution for 20 minutes while rocking (VWR analog rocker 10127-876), in the dark and on ice ...
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bioRxiv - Biophysics 2024Quote: ... The sections were then incubated overnight at 4 °C with CRB2 rabbit polyclonal antibody (1:100, VWR, AP5724B) and nephrin guinea pig polyclonal antibody (1:200 ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then split into a negative control (no antibiotic) or antibiotic treatment (2 mL of culture per each treatment, using plastic Falcon tubes, VWR® Cat. No. 352059). After addition of the antibiotic from concentrated stocks ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μL cell suspension was loaded on YES agarose pad (2% agarose) and covered with 22 x 22 mm glass coverslip (VWR #631-0125, thickness: 1.5). Time-lapse imaging of mNG-cdc8 cells were performed at 30 °C with acquisition of 16 z-slices ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfected cells from 2 wells (technical duplicates) were seeded into 250 mL cell culture flasks with vented caps (10062-860, VWR International, Brisbane, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...
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bioRxiv - Microbiology 2022Quote: ... puncturing the central mineralized region at different locations with a disposable antistatic microspatula with a diameter of 2 mm (VWR international, Radnor, USA, PA). The samples were then sandwiched between two discs of 3 mm diameter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cultures were seeded at 104 cells/mL in 80 mL of 15/2 Media in 300 cm2 vented tissue culture flasks (VWR, Cat. No. 10062-884) and grown at 22°C.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Deer IgG (H+L) secondary antibody (95058-328, VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Genomics 2020Quote: Library preparation for each CUT&RUN antibody condition was done with a KAPA Hyper Prep Kit (VWR, 89125-040) with the PCR amplification settings adjusted to have simultaneous annealing and extension steps at 60 °C for 10 seconds ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were washed in PBST and followed by incubation with HRP-conjugated secondary antibody (goat antirabbit, VWR/Jackson Immunoresearch). Then avidin–biotin peroxidase complex (Vectastain ABC kit ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The membranes were washed 3x in PBST at RT and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (VWR, Amersham ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Gt-680 925-68074, Ms-800 926-32212 Gt-800; 926-32214) (VWR) at 1:20,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Afterward membranes were washed 3X with TBST and incubated with HRP-conjugated secondary antibodies for 1h and then were washed again and revealed by ECL (VWR) chemiluminescence.
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% milk and then probed with the indicated primary antibodies diluted in 5% bovine serum albumin (VWR). Blots were imaged after incubation with appropriate primary and secondary antibodies by scanning on an Odyssey IR imager (LI-COR) ...
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bioRxiv - Biophysics 2021Quote: ... were washed with 200 μL PBS/0.2 mM DDM and incubated with 4 μg RHO 1D4 antibody (University of British Columbia) in 50 μL PBS/0.2 mM DDM on a ferris wheel (VWR, 30 min). After washing the beads PBS/0.2 mM DDM (with 200 μL) ...
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bioRxiv - Neuroscience 2024Quote: Detection with 3,3′-diaminobenzidine (DAB) was performed using horseradish peroxidase labeled secondary antibodies and DAB Quanto chromogen and substrate (VWR, Radnor, PA).
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight at 4°C followed by a goat anti-rabbit IgG secondary antibody conjugated to HRP (VWR, cat. no. 102645-182). F1β signal was then captured using the same method.
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed three times for five minutes with 0.02% Tween in PBS and incubated for one hour at RT with HRP-linked antibodies – anti-rabbit (VWR, cat.no. NA934) or anti-mouse (VWR ...
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bioRxiv - Physiology 2024Quote: ... the cells were washed twice with 1x DPBS and stained with secondary antibodies: Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat #102649-732, 1:300, VWR), and Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... MA). Anti-rabbit secondary antibody Alexa Fluor® 488 anti-IgG donkey (cat. #102649-730) was purchased from VWR (Radnor, PA).
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bioRxiv - Systems Biology 2024Quote: ... All antibodies were diluted at 1:100 in FACS buffer [PBS with 10% fetal bovine serum (FBS, VWR, MDTC35-016-CV) containing 1% Penicillin-Streptomycin solution (P/S ...
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... PU Topounits samples were fixed in 10% v/v formal saline and labelled with antibodies targeting CD45 (pan-leukocyte marker; VWR violetfluor 450), and the membrane-selective dye FM1-43 (Thermofisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were mounted on a thin (∼1-2x lab tape thickness) 2% agar pad on a glass slide (VWR VistaVision, 3 inches x 1 inch x 1 mm) using a hand pulled borosilicate glass capillary pipette (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... transfected and infected cells were stained with an anti-N rabbit polyclonal antibody followed by incubation with secondary 594-conjugated Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX). Nuclei were stained with DAPI (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 4-cell stage embryos were mounted on a thin (∼1-2x lab tape thickness) 2% agar pad on a glass slide (VWR VistaVision, 3 inches x 1 inch x 1 mm) using a hand-pulled glass pipette (VWR Pasteur Pipette ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...