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Citations for VWR :
451 - 500 of 523 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was then incubated in 4% PFA/PBS overnight and either immersed in serial solutions of 15% and 30% sucrose/PBS and embedded in OCT (VWR Chemicals) for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica) ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... fixed breast samples were impregnated with 30% Sucrose solution in 1X PBS for at least 48h and embedded in OCT (VWR, #361603E). 70 μm-thick sections were incubated with 0.5% Triton-blocking buffer (5% FBS and 2% BSA in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Developmental Biology 2020Quote: ... each embryo was positioned in a sagittal orientation in a tissue base mold (Sakura, 4162) in optimal cutting temperature compound (OCT) solution (Sakura, 4583) and frozen in a dry ice isopropanol (VWR, 20842) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Cancer Biology 2021Quote: Glass-bottom 6-well dishes (#0 thickness, IBL, #220.200.020) were treated with a bind-silane solution consisting of PlusOne Bind-Silane (VWR, #17-1330-01) and acetic acid (Pancreac Quimica ...
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bioRxiv - Neuroscience 2023Quote: ... brains were kept for 1 hour in a 1:1 solution of 30% sucrose and Optimal Cutting Temperature Medium (OCT, VWR International) at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... and excess gel was washed away with 5 Milli-Q purified water washes and 5 Dulbecco’s Phosphate Buffered Saline (DPBS) solution (VWR L0119-0500) washes ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Molecular Biology 2024Quote: Arabidopsis thaliana Col-0 seeds were surface sterilized for 10 min in a 50% bleach solution with Tween 20 (VWR 0777A) followed by three washes with sterile dH2O ...
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bioRxiv - Microbiology 2024Quote: ... The pigmented extract was dissolved in 99.5% methanol and then 10 mL of the solution was poured into a clean cuvette and inserted into a P9 double beam UV-visible spectrophotometer (VWR, China) which directs a beam of light through the sample using methanol as negative control ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... a resazurin solution was prepared by dissolving one tablet of Resazurin (Resazurin Tablets for Milk Testing; Ref. 330884Y VWR International Ltd) in 30 mL of sterile PBS (phosphate buffered saline) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination.
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2022Quote: ... 50 µL of the agarose solution was then transferred over to each well of the 96 well U bottom plate (10062-900, VWR, PA, USA) then let solidify at room temperature (RT) ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Bioengineering 2024Quote: ... the peptide solutions were pH adjusted to 7 and sterile filtered using 0.2 μm cellulose acetate sterile syringe filters (VWR International, Radnor, PA) under sterile conditions ...
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bioRxiv - Bioengineering 2022Quote: ... cells for both batches were seeded onto the material at a confluence comprised between 50% and 60% on fibronectin coated (15 μg/ml in PBS buffer solution) 18-mm round glass coverslip (VWR, Radnor, USA). The cells were maintained in incubator at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903], 10mM KH2PO4 [VWR, 470302] ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Bioengineering 2024Quote: ... was defined by completely demineralizing a 2% gelatin solution by adding an abundance of mixed bed ion exchange resin Amberlite IRN-150 (VWR, 40992.A9) to the gelatin ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was passed through a 0.22 µm filter before 0.5 ml was combined with 2 ml of Patton-Reeders solution (0.105 mM calconcarboxylic acid, VWR, in 0.73 M NaOH) and vortexed briefly to mix ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Plant Biology 2021Quote: ... Membrane protein samples containing 1 μg total Chl were solubilized and separated by electrophoresis on a 10 % SDS-polyacrylamide gel using Next Gel solutions and buffers (VWR, Radnor, PA, USA). Proteins were transferred to Immobilon-P PVDF membranes (MilliporeSigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were weighed and rinsed in blood bank saline solution (150 mM NaCl) and blotted on filter paper (VWR, Radnor, PA, 28298–020). The process of dissection and tumor preparation took < 3min ...
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bioRxiv - Neuroscience 2023Quote: ... slides that included the region of interest were washed with PBS before quenching with a solution of 50% methanol (VWR, Radnor, PA, USA) and 3% hydrogen peroxide (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Cell Biology 2024Quote: ... cells were passaged 1:10 to a new 96-well Matrigel-coated plate for gDNA extraction with 20 μL of Quick Extract gDNA lysis solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA). The remaining cells were overlaid with 50 μL of 20% mTeSR1 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder of the cells were spun and resuspended in 20 μL of Quick Extract gDNA lysis solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA) and prepared for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... and stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was extracted from lysed samples using the Quick Extract DNA Extraction Solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA), following the protocol provided by the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... the tubes and multiplexers of all liquids are flushed for cleaning with first 1.4 % sodium hypochlorite in aqueous solution (7681-52-9, VWR International, Radnor, PA, USA) diluted with ultrapure water ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Microbiology 2021Quote: ... They were then stained with 2% uranyl acetate in an aqueous solution (Taab Laboratories, Berkshire, UK) for 15 minutes followed by Reynolds lead citrate stain (VWR International Ltd, Leicestershire, UK) (Reynolds ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −20 °C in an anti-freeze solution (30 % glycerol [Merck Millipore, Germany], 30 % ethylene glycol [VWR International, USA] and 10 % TBS) until further processing ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...