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Citations for VWR :
451 - 500 of 1369 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at room temperature on a shaker in presence of 3 μg LysC Endopeptidase (VWR). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μm-thick sections were obtained and counterstained with Liquid-Stable Nuclear Fast Red (VWR Life Science AMRESCO).
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bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
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bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer; VWR, Radnor, PA, United States). The supernatant was transferred to a clean tube ...
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bioRxiv - Microbiology 2024Quote: ... were added by incubating samples for 3.5 hours at 37°C in 3% BSA (VWR Cat# 97061-422) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min in 1x PBS and mounted onto SuperFrost slides (VWR, #48311- 703) using VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... the sample was applied onto polyethersulfone membrane centrifugal filters with 3 kDa cutoff (VWR International GmbH, Darmstadt, Germany) to concentrate this fraction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cultures were seeded at 104 cells/mL in 6 mL of 25% RA in 25cm2 vented tissue culture flasks (VWR Cat. No. 10062-874) and grown at 27°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Co-culture experiments with PC3 WT and GGT1+ cell lines were performed using 6-well (24 mm) PET inserts with 0.4 µm pores (VWR 76313-902 or Corning 354570). For these experiments ...
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bioRxiv - Physiology 2022Quote: ... cells grown on coverslips were washed with PBS and fixed with 4% paraformaldehyde (VWR Chemicals, Spain). Then ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubating in 200 μL lysis buffer (4% (wt/vol) sodium dodecyl sulfate (VWR International), 0.5% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... cryopreserved in 30% sucrose overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (14 μm ...
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bioRxiv - Microbiology 2022Quote: ... We filter-sterilized the media using 150-mL 4-μm vacuum filtration tops (VWR: 10040-444) after adjustment ...
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bioRxiv - Microbiology 2022Quote: ... in 1x PBS and incubated with shaking overnight at 4 °C (Digital Platform Rocker Shaker, VWR). Following overnight fixation ...
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bioRxiv - Genetics 2023Quote: ... Fixed flies were then placed in warm 4% agarose solution inside a 15mmx15mmx5 mold/adaptor (VWR) and left on ice for 30min ...
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bioRxiv - Biophysics 2023Quote: ... The fixed brains were then placed in agarose (4% solution, #9012366, VWR Life Science, Hannover, Germany), before cutting to the desired thickness (100-200 µm ...
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bioRxiv - Neuroscience 2023Quote: ... mouse brains were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed with PBS once and fixed using 4% Paraformaldehyde (VWR, Cat. # 97061-850) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips were washed three times with PBS buffer and fixed using 4% buffered formaldehyde (VWR, 9713.1000) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were cut with a cryostat and 3 sections of 20 µm were placed on each microscope glass slide (VWR). Sections were fixed with acetone for 10 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: 3 biological replicates of each isolate were grown overnight in 96-deep-well plates (VWR International, cat #10755-248) in LB media ...
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bioRxiv - Biochemistry 2021Quote: Custom Eppendorf tubes for extract recovery were created by cutting approximately 3 mm from the bottom of a standard 0.5 mL Eppendorf tube (VWR). The tubes were then soaked in 70% ethanol to remove small molecule contaminants ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed 3 times in PBS before blocking for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).
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bioRxiv - Molecular Biology 2024Quote: Purified chromosomes 3 or 19 were cytocentrifuged (Cytospin3, Shandon) at 163 g for 10 min onto Polysine slides (VWR). After mounting with Vectashield containing DAPI (Vector Laboratories) ...
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bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
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bioRxiv - Biophysics 2022Quote: ... Cell aggregates on top of the droplets were transferred to an electroporation cuvette (4 mm gap, VWR). Three square wave pulses of 1 kV for 70 μs were applied with 1 s intervals between each pulse using an ECM 2001 Electro Cell Manipulator (Harvard Apparatus ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... an aqueous solution of sodium dodecyl sulfate (SDS; concentrations: 2% or 4%; Cat. No. 97064-472, VWR) was instilled directly into the trachea via an inlet cannula ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in cold PBS/0.04% BSA and fixed by adding 4 volumes 100% Methanol (VWR) and incubation for 30 min at -20 °C ...
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bioRxiv - Bioengineering 2022Quote: ... VWR Spinbar Micro Stir Bars and 4-ml Amber Borosilicate Glass sample vials were purchased from VWR.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Organoids were fixed in 4% PFA and embedded in Tissue-Tek® O.C.T (VWR, cat # 25608-930). Slides of cryosectioned organoids were blocked in 5% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% Triton X-100) on ice for 2 min and immediately fixed in formaldehyde 4% (VWR) for 10 min at the room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The inoculum was then streaked onto XLT-4 containing 64 µg/mL nalidixic acid (VWR; Lot:21J285302) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 1e8 bacteria from each strain were pelleted and resuspended in 500 μL 4% paraformaldehyde (VWR, AAJ61899-AP) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were transcardially perfused with cold (4 °C) sterile saline and then 10% formalin (VWR, Radnor, PA). The brains of the mice were extracted from the skull ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...