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Citations for Zymo Research :
51 - 100 of 400 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: RNA purification and on-column DNase I treatment were performed using a Quick-RNA Fungal/Bacterial Miniprep kit and DNase I set (Zymo Research) following the manufacturer’s recommendations with minor modifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and potential PCR inhibitors were removed with the OneStep-96TM PCR Inhibitor Removal Kit (Zymo Research). RNA concentrations were quantified with the Qubit RNA Assay kit (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo, D6030), per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... After PCR product purification (Zymo Research), the fragments of interest were inserted into linearized ZP446 using Gibson Assembly ...
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bioRxiv - Genetics 2020Quote: ... DNA was digested in columns by adding a mixture of 5 μl RNA Lysis Buffer 1 from the kit andl DNase I and 75 μl RNA Lysis Buffer 1 from the kit andl DNA Digestion Buffer from DNase I Set kit (Zymo Research E1010) followed by a 15-minute incubation at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: RNA from K562 and Nalm6 cells was isolated using the NucleoSpin RNA Mini kit (Macherey Nagel) followed by DNase-digestion with the DNase I set (Zymo Research, #E1010) and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... PCR inhibitors were then removed from the extracted DNA using the OneStep PCR inhibitor removal kit (Zymo) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013, Zymo Research Co.). Depending on the amplicon ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research, Irvine, CA), and the recovered DNA fragments were ligated together to form closed circular DNA using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Neuroscience 2019Quote: ... and via RT-qPCR with a next generation sequencing (NGS) library quantification kit (Zymo Research, Irvine, CA). RNA sequencing (1 lane ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments were amplified using overhang primers (Supplementary table 1) and purified using DNA Clean & Concentrator kit (Zymo Research). The pUC19-iTol2 backbone was digested with BamHI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The three PCR fragments were purified (Zymo DNA Clean and Concentrator kit ...
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bioRxiv - Plant Biology 2020Quote: ... and primers with BsaI sites as overhang (Table S2) and purified using the DNA Clean&Concentrator Kit (ZYMO RESEARCH). PCR fragments and the acceptor vector were incubated in Buffer G (Thermo SCIENTIFIC ...
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bioRxiv - Bioengineering 2021Quote: Colony PCR was performed on bacterial cultures and the PCR product purified using the DNA Clean and Concentrator kit (Zymo). Purified PCR products were sequenced at Genewiz (USA).
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Molecular Biology 2023Quote: The PCR products containing the aforementioned various RNA constructs were cleaned using a PCR DNA clean up kit (Zymo Research), and used in Gibson assembly reactions (5x Gibson buffer ...
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bioRxiv - Microbiology 2024Quote: ... The three PCR fragments were purified (Zymo kit) and used in a 1:1:1 molar ratio Golden Gate assembly reaction with AarI (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The three PCR fragments were purified (Zymo kit) and used in a 1:1:1 molar ratio Golden Gate assembly reaction with AarI (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DpnI-treated inverse PCR vector and PCR product inserts were column purified using a DNA Clean and Concentrate Kit (Zymo Research). Inserts were added with 2-fold molar excess to 75 ng of the vector ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were purified (Zymo DNA Clean & Concentrator kit) and then combined ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate) and digested with EcoRI and BamHI alongside digestion of the base plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate). Successful Gibson Assembly reactions occurred using a 7:1 molar ratio of insert:backbone.
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were column-purified (Zymo Research, Irvine, CA) and eluted in 30 μL ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were column cleaned (Gel cleanup kit, Zymo), and pooled based on relative concentration ...
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bioRxiv - Cell Biology 2021Quote: ... and purified with a PCR purification kit (Zymo Research). TRIzol™ (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and purified using a PCR purification kit (Zymo Research), and then used for in vitro transcription with the MEGAshortscript T7 kit (Ambion) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of each module (PCR product + Zymo Shield) (6 in total ...
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bioRxiv - Microbiology 2020Quote: ... Amplified insertions were PCR purified (DNA purification kit, Zymo) and subjected to Sanger sequencing by MCLAB ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were purified (Zymo Research Clean and Concentrator kit) and pooled for sequencing on an Illumina MiSeq with 150 bp single end reads ...
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bioRxiv - Plant Biology 2021Quote: ... and Zymo OneStep PCR Inhibitor Removal Kit (D6030, Zymo) to obtain high quality genomic DNA ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were column cleaned (Gel cleanup kit, Zymo), and pooled based on relative concentration ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of 2X ZymoTaq PCR Mix (Zymo Research), PCR primers ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was purified using a kit (Zymo Research DNA Clean & Concentrator™ Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The ITS regions were amplified by qPCR using ITS-specific primers provided by the Femto(tm) Fungal DNA Quantification Kit (Zymo Research) to quantify the relative abundance of fungal DNA following the manufacturer’s recommendations for the three biological replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Microbiology 2022Quote: ... or by RT-qPCR (IDT technologies, Charité kit) after RNA extraction with TRI reagent and Direct-Zol RNA miniprep kit (Zymo Research).
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bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were purified using DNA Clean & Concentrator Kit (Zymo) and sent for Sanger sequencing to obtain trace files (Genewiz) ...