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Citations for Zymo Research :
351 - 400 of 400 citations for mmu mir 378c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The two backbone halves and the desired antibiotic resistance marker were PCR purified using the DNA Clean & Concentrator kit available from Zymo Research ...
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bioRxiv - Synthetic Biology 2023Quote: ... for DNA receiver PCR product purification and Quick-RNA 96 Kit for guide RNAs (gRNAs) purification were purchased from Zymo Research (Irvine ...
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bioRxiv - Synthetic Biology 2023Quote: ... A maximum of 96 PCR reactions were pooled at a time and cleaned with Zymo-Spin II columns (Zymo Research). Pooled samples were sequenced with the NGSelect Amplicon sequencing service of Eurofins Genomics.
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bioRxiv - Genetics 2023Quote: ... The PCR products were then pooled at equimolar ratios and cleaned using the DNA Clean and Concentrator Kit (Zymo Research), followed by gel extraction ...
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bioRxiv - Microbiology 2024Quote: ... The size of the PCR products was verified by agarose gel electrophoresis and purified by using the Zyppy extraction kit (Zymo). The amplified PCR products were digested with BamHI and XhoI and ligated with similarly digested pCTX-1-lux ...
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bioRxiv - Biophysics 2020Quote: ... The completed PCR reactions were pooled and ethanol-precipitated prior to purification using a DNA Clean & Concentrator Kit (DCC-25, Zymo Research). The concentration of the labeled 197-bp PCR products was determined by UV absorbance ...
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bioRxiv - Molecular Biology 2022Quote: ... We followed the PCR with an agarose gel extraction and a column purification using the Oligo Clean & Concentrator kit (Zymo Research). The DNA template was then in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...
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bioRxiv - Cell Biology 2019Quote: ... DNA fragments used for probe library generation were amplified by PCR and recovered by DNA Cleanup kit (D4014, Zymo research, U.S.A.). The amplified probe library could serve as templates for Tn5-FISH probes ...
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bioRxiv - Bioengineering 2019Quote: Knock-in at the AAVS1 locus was verified through PCR on genomic DNA extracted with a Quick-DNA kit (D3024, Zymo Research). For the positive knock-in screen ...
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bioRxiv - Genetics 2020Quote: ... all PCR products were cleaned using a Zymo Research ZR-96 DNA Clean & Concentrator™-5 (Zymo Research, Irvine, California, USA). Samples were eluted in 50µl of DNA free water.
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bioRxiv - Microbiology 2021Quote: ... after which total RNA was extracted as described in “Total RNA extraction and qRT-PCR.” Following RNA cleanup (Zymo Research; #R1017), 2 μg total RNA for each sample was depleted for rRNA using a Ribominus Transcriptome Isolation Kit (Yeast and Bacteria ...
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bioRxiv - Immunology 2020Quote: PCR testing for SARS-CoV-2 was carried out on respiratory specimens mixed 1:1 in DNA/RNA Shield (Zymo Inc) using an in-house Clinical Laboratory Improvement Amendments (CLIA ...
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Microbiology 2019Quote: ... and PCR products were extracted and purified from agarose gels using the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). The Zyppy Plasmid Miniprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were analyzed via agarose gel electrophoresis and the clean-up was either done by bead purification with HighPrepTM PCR (MAGBIO) or column purification with the DNA Clean & ConcentratorTM kit (Zymo Research). The purified samples were send for Sanger sequencing (Eurofins Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were isolated from 0.8% agarose gels using the Zymoclean™ Gel DNA Recovery Kit (ZYMO Research, Irvine, CA, USA). Enzymes Dream Taq Master Mix ...
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bioRxiv - Immunology 2021Quote: ... Products were checked on an agarose gel and cleaned-up through a PCR-clean up kit (DNA Clean and Concentrator-5, Zymo, D4013), followed by a secondary PCR to add illumina indexes (SFig ...
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bioRxiv - Molecular Biology 2022Quote: ... Qiagen DNeasy Blood and Tissue Kit and post-extraction inhibitor removal with the Zymo Research OneStep™ PCR Inhibitor Removal Kit (QBT-ZYMO), 4 ...
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bioRxiv - Microbiology 2022Quote: ~1 kb products from the Pan-Entamoeba PCR above were gel purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research) and submitted to the UW-Madison Biotechnology Center for Sanger Sequencing using the amplification primers described above ...
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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In vitro impact of fluconazole on oral microbial communities, bacterial growth and biofilm formationbioRxiv - Microbiology 2023Quote: ... and quantified by real-time PCR using Zymo DNA quantification kits for bacteria/fungi (Femto Bacterial and fungal DNA Quantification kit, Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
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bioRxiv - Microbiology 2023Quote: ... the same PCR fragment used for Sanger sequencing was purified using a ZymocleanTM Gel DNA Recovery Kit (Zymo Research, cat# D4002). DNA (∼1 µg ...
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bioRxiv - Genomics 2024Quote: ... DNA was eluted in 105 µL RNAse free H2O and was further cleaned using OneStep PCR Inhibitor Removal Kit (Zymo Research). Concentrations for all DNA samples were measured using Qubit 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2024Quote: ... Products were checked on an agarose gel and subjected to a PCR-clean up step (DNA Clean and Concentrator-5, Zymo, D4013). A second PCR (PCR2 ...
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bioRxiv - Microbiology 2024Quote: ... The vector and PCR products were digested with restriction enzymes and band-purified (vector) or cleaned (PCR products) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) or the DNA Clean & Concentrator-5 kit (Zymo Research) ...
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bioRxiv - Immunology 2019Quote: ... PCR products were applied to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: ... Isolated gDNAs were further prepared and cleaned up using a OneStep™ PCR Inhibitor Removal Kit according to manufacturer instructions (Zymo Research, D6030).
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bioRxiv - Cell Biology 2022Quote: IRES-Venus and human SNCA fragments were amplified by PCR (MJ Research, PTC-200 Peltier Thermal Cycler) and purified using DNA Clean & Concentrator™-5 Kit (Zymo Research, D4003) to provide 20 μl of eluted DNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of the PCR product that contained the specific modules were stored in Zymo DNA/RNA shield (Zymo Research Cat.No R1100-50) in the freezer at -20 °C ...
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bioRxiv - Microbiology 2020Quote: ... the nucleic acids were resuspended in DEPC-treated nuclease-free water purified with the OneStep PCR Inhibitor Removal Kit (Zymo Research, Irvine, USA), then split into a fraction for DNA and one for RNA ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Undigested bands corresponding to mutant PCR products were gel extracted with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Cat. No./ID: D4007), then cloned into pJET vectors (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... Single viable CFSElow CD3+ CD8− CD4+ T cells were sorted into 96-well PCR plates containing DNA/RNA Shield (Zymo Research, Irvine, CA, USA) using FACSAria II and Diva Software (BD Biosciences ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was eluted in 60 μl elution buffer and further purified by using the OneStep PCR Inhibitor Removal Kit (ZymoBIOMICS, Zymo Research, Irvine, CA, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was prepared using the ZymoPure II Plasmid Midiprep Kit followed by an ethanol precipitation and linear expression templates were prepared by PCR and subsequent cleanup using the Zymo DNA Clean and Concentrator (Zymo Research, Irvine, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The barcoded PCR products were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research 11-301, 11-303) and quantified by Qubit 1× dsDNA HS assay kits (ThermoFisher Q32851) ...
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bioRxiv - Molecular Biology 2022Quote: ... The barcoding PCR reactions were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research Cat. #: 11-301 and 11-303) and quantified by Qubit 1x dsDNA HS assay kits (Thermo Fisher Scientific ...