Labshake search
Citations for Zymo Research :
51 - 100 of 110 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from all water samples using the ZymoBIOMICS TM 96 MagBead DNA kit (Zymo) and a KingFisher Flex instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembly products were then cleaned and eluted in 6 μL water (Zymo Clean and Concentrate). Then ...
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bioRxiv - Genomics 2024Quote: ... Gibson assembled products were then cleaned and eluted in 10 μL water (Zymo Clean and Concentrate). 1 μL of cleaned product was then added to 25 μL NEB-10β E ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was purified in 10 μl water using the Oligo Clean and Concentrator™ kit (Zymo). Next ...
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bioRxiv - Microbiology 2022Quote: ... DNA from pond water samples was cleaned using the Genomic DNA Clean & Concentrator kit (D4010, Zymo Research), quantified with the Qubit dsDNA BR Kit (Q32850 ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL ...
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bioRxiv - Cell Biology 2023Quote: ... followed by two rounds of DNA digestion using the DNA-free kit (Zymo). Samples were analyzed with a nanodrop 2000 and RNA Qubit to ensure sample concentrations were above 150 ng total for library construction ...
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bioRxiv - Immunology 2023Quote: ... Endotoxin-free plasmids were purified using the ZymoPURE II Plasmid Maxiprep Kit (Zymo).
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were resuspended in 500 µL water and transferred into ZR Bashing Bead Tubes (Zymo Research, CA). The ZR Bashing tubes were centrifuged for 1 minute at 8000g to discard the water ...
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bioRxiv - Cell Biology 2019Quote: ... the rRNA-free eluate was again column-purified (RNA Clean & Concentrator-5, Zymo Research). RNA libraries were generated using the NEBNext Ultra RNA Directional Library (New England Biolabs) ...
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bioRxiv - Systems Biology 2022Quote: ... DNA-free RNA was extracted from cell using DIRECTzol kit (Zymo Research, Irvine, CA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Each 200 mL of culture was purified in Endotoxin Free MaxiPrep Reactions (Zymo #D4203) and eluted in 0.4 mL elution buffer per column.
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bioRxiv - Cancer Biology 2024Quote: ... vectors were amplified by endotoxin-free Giga prep (Qiagen 12391 or Zymo Research D4204). NEB Stable chemically competent E ...
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bioRxiv - Neuroscience 2024Quote: ... DNA from several clones was recovered with an endotoxin free midi-kit (Zymo D4202). This allows for consistently obtaining low recombination rates detected by PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... This reaction was purified and concentrated in 12 μl water using the DNA Clean & Concentrator™ kit (Zymo, #D4033) and transformed into electrocompetent SURE 2 cells (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... One negative water control and one mock community DNA control (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA) were included on each plate ...
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bioRxiv - Genomics 2021Quote: ... DNA was then eluted in 20 μl deionized water using the Zymo DNA Clean and Concentrator kit (Zymo, D4033). 10 μl of eluted DNA was used for library preparation exactly as described for CUT&RUN ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was then eluted in 20 µl deionized water using the Zymo DNA Clean and Concentrator kit (Zymo, D4033). 10 µl of eluted DNA was used for library preparation exactly as described for CUT&RUN ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Added water to volume of 50 μL and cleaned up the RNA (Zymo RNA clean and concentrator -5 # R1013) and eluted in 15 μL water.
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bioRxiv - Molecular Biology 2019Quote: ... DNA free total and mRNA were prepared using Direct-zol RNA kit (R2060, Zymo Research) and GenElute™ mRNA Miniprep kit (MRN10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and prepared as endotoxin-free midipreps using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research)
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bioRxiv - Genetics 2020Quote: ... DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research, cat. D4208T).
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... Endotoxin-free plasmid library DNA was prepared using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research). To clone the library of gRNA pairs ...
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bioRxiv - Genetics 2020Quote: ... 100 mg of thawed cell pellets were washed three times in 1 mL deionized water and extracted using Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). A total of 2.4 μg of purified gDNA was fragmented by sonication to average size of ~350 bp in four separate aliquots using a Diagenode Picoruptor ...
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bioRxiv - Microbiology 2023Quote: ... one for each extraction batch) using commercially available kits optimized for water and tissue-based microbial communities (Quick DNA Fecal/Soil Kit, Zymo Research). DNA extract was roughly quantified and purity-checked with a Thermo Scientific™ NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... insect legs were mechanically crushed and resuspended in 50 μl of sterile water and the genomic DNA (gDNA) was extracted using a Quick-DNA Universal kit (Zymo research, USA). 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega ...
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bioRxiv - Genomics 2020Quote: ... Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation) from 50 mL Mach1 (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... All plasmids were prepared with the Endotoxin-Free Zymopure Midiprep or Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were prepared for injection using ZymoPURE II endotoxin-free midiprep kit (Zymo Research Corp, Irvine, CA). Cas9/sgRNA and donor plasmids were diluted to concentrations of 300ng/μl for injection ...
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bioRxiv - Immunology 2023Quote: The total RNA samples (1 µg each) were further purified with DNA-free RNA kit (Zymo Research). All of the samples were subjected to rRNA removal using the QIAseq FastSelect -rRNA HMR Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA sample was then extracted with chloroform and the water layer was further purified using DNA Clean & Concentrator kit (Cat. D4029, Zymo Research, California, U.S.). The samples were then sent for next generation sequencing using the BGI-Seq platform (BGI ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Genomics 2023Quote: ... RNA isolates were treated with DNase1 and cleaned using the DNA-free RNA Kit (Zymo Technologies, Irvine, CA). RNA quality was assessed by use of a fragment analyser (Advanced Analytical Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... PBS cell suspension was pipetted to a pre-chilled to 1.5 mL RNase-free snap lid tube (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... DNA-free viral RNA was isolated from the viral supernatants using Direct-Zol RNA Microprep kit (Zymo research) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
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bioRxiv - Bioengineering 2023Quote: ... Cells and released probes were centrifuged to pellet cells and the supernatant was purified with spin columns and DNA was eluted in 20ul of water (Zymo ssDNA/RNA clean & concentrator). 1 μL of purified samples were loaded into each 20 μL reaction of a qPCR with 0.3 μM primers (Supplementary table S9 ...
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bioRxiv - Microbiology 2024Quote: ... Every extraction batch of 96 samples included one water blank as a negative control and one mock community aliquot (Zymo Research; Cat. No. D6300) as a positive control ...
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bioRxiv - Neuroscience 2023Quote: ... Free RNAs were extracted using phenol/chloroform and purified with the RNA Clean & Concentrator-5 kit (Zymo Research, R1015).
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bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). The Bxb1 recombinase expression vector (pCAG-NLS-HA Bxb1 ...
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bioRxiv - Genomics 2021Quote: ... Chronotype was assessed by computing mid-sleep time on free days (MSF).2 Participants’ stool samples were collected using DNA/RNA Shield Fecal Collection tubes (Zymo research). Sample DNA extraction was performed using the PureLink Microbiome DNA Purification Kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... 1.5 µg of genomic DNA in 30 ml ddH2O was digested to free nucleosides using 5 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). Transient expression of individual variants for western blots was carried out using a distinct set of expression vectors encoding the untagged CFTR variant cDNAs in the context of a pCDNA5 expression vector ...
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bioRxiv - Genomics 2023Quote: Total RNA (∼1 µg) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs from the left ventricular free wall were extracted by Trizol LS and purified and DNaseI treated using Directzol RNA miniprep kit (Zymo, R2052). Purified RNAs (1.5 µg ...
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bioRxiv - Genomics 2023Quote: ... Total RNA (∼700 ng) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...