Labshake search
Citations for Zymo Research :
301 - 317 of 317 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from the input library and from three biological replicates of phage exposed and unexposed samples using a ZymoBIOMICS™ DNA Miniprep Kit (Zymo Research), following the manufacturers protocol.
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bioRxiv - Genomics 2019Quote: ... NGS libraries consisted of tagmented DNA reisolated from excised regions of gel lanes using a Zymoclean Gel DNA Recovery® kit (Zymo Laboratories) (13).
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bioRxiv - Bioengineering 2022Quote: Plasmid DNA was extracted from frozen glycerol library aliquots using a Zyppy Plasmid Miniprep Kit according to manufacturer instructions (Zymo, Cat# D4036). After performing assembly selections on the double mutant libraries ...
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bioRxiv - Genomics 2022Quote: ... DNA extracts from each experimental replicate without standards or phage HM1 were cleaned up prior to library preparations with the Zymo Genomic DNA Clean and Concentrate-10 kit (Cat. No. D4011, Zymo Research Corporation). Long read libraries were prepared with the Ligation Sequencing Kit (Cat ...
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bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from line with Bon GLKD driven by MT-Gal4 (and control flies that express an shRNA against the white gene) was depleted of ribosomal RNA with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 40–60 minutes and demethylated using rtStar tRNA-optimized First strand cDNA synthesis kit following manufacturer’s protocol with intervening RNA cleaning steps (Zymo RNA Clean and Concentration Kit). For the annealing reaction ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA samples were prepared for targeted sequencing with the Quick-16S NGS library Prep kit and 16S primer set V3-V4 (Zymo Research Irvine, CA). The final library was sequenced on Ilumina MiSeq with a V3 reagent Kit (600 cycles) ...
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bioRxiv - Biochemistry 2021Quote: ... which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research, Irvine, CA). An analysis of our final DMS data suggest this library contains at least 7,614 of 7,686 possible variants ...
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bioRxiv - Microbiology 2019Quote: ... we also produced ten separate V3-V4 16S rRNA gene amplicon libraries from the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA) using the 2-Step library preparation method ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Molecular Biology 2022Quote: Single-stranded cfDNA methylation sequencing libraries were prepared as above after input cfDNA was converted using either the EZ DNA Methylation Gold kit (Zymo Research, cat. No. D5006) or the EM-seq Conversion Module (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was then used to prepare the sequencing library and final qPCR fluorescence readings were pooled together according to equal molarity and the final pooled library was cleaned using the Select-a-Size DNA Clean & Concentrator™ (Zymo Research, Irvine, CA), and quantified with TapeStation® (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... To characterize the microbial diversity based on 16S rRNA gene sequences we used the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine CA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Prior to library preparation the DNA concentration of each sample was normalized and 20pg of spike-in gDNA was added (Zymo Research High Bacterial Load Spike-in), which contains gDNA from the bacterial species Imchella halotolerans and Allobacillus halotolerans ...