Labshake search
Citations for Zymo Research :
1 - 50 of 6078 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). For the interaction of TaNF-YA3-D ...
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bioRxiv - Genetics 2024Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). The coding sequences of TabHLH27-A1 were cloned into the prey vector (pGADT7) ...
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bioRxiv - Microbiology 2024Quote: ... the two fragments were purified (Zymo kit) and used in a 1:1 molar ratio Golden Gate assembly reaction with BsaI-HF2 (NEB ...
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bioRxiv - Genomics 2020Quote: ... amplification and library construction procedures were performed with Pico Methyl-SeqTM Library Prep Kit (Zymo Research) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... subsequent to spheroblast construction using 10 U of Zymolyase (ZYMO Research) per sample for 30 mins at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... cleaned across two columns from the DNA clean and concentrator kit (Zymo), and eluted twice with 10 μL H2O ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA-RNA hybrid was purified using ChIP DNA Clean & Concentrator Kit (Zymo Research Corporation, Irvine, CA, USA), RNaseH treated and circularized ...
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bioRxiv - Microbiology 2022Quote: ... RNA sequencing library construction and ribosomal RNA depletion were performed using the Zymo-Seq RiboFree™ Total RNA Library Kit (Zymo Research). Paired-end (150 bp ...
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bioRxiv - Cell Biology 2023Quote: ... followed by two rounds of DNA digestion using the DNA-free kit (Zymo). Samples were analyzed with a nanodrop 2000 and RNA Qubit to ensure sample concentrations were above 150 ng total for library construction ...
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bioRxiv - Genetics 2024Quote: ... Seventy-two hours after treatment RNA was extracted (Zymo Research Quick RNA mini prep kit), followed by RT-qPCR analysis employing gene probes (Supplementary Table S5 ...
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bioRxiv - Genomics 2019Quote: ... or by sodium bisulfite using two commercially available kits: BS (1)EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research) and BS (2 ...
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bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
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bioRxiv - Molecular Biology 2021Quote: We isolated RNA from each lineage (two replicates each) using the Direct-zol Miniprep RNA kit (Zymo) with DNase treatment ...
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bioRxiv - Cell Biology 2020Quote: ... mixed with two volumes of 100% ethanol and purified using an RNA Clean & Concentrator-25 kit (Zymo Research) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was extracted from the two transformed lines of 293T cells using RNA Miniprep kit (Zymo Research). For cDNA to be used in RT-PCR ...
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bioRxiv - Cancer Biology 2022Quote: Two nanograms of genomic DNA was bisulfite converted using the EZ DNA methylation kit (Zymo Research, Orange, CA) and eluted in 11 μl of TE buffer ...
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bioRxiv - Microbiology 2019Quote: ... in two technical replicates on 50 ng template DNA extracted with a YeaStar Genomic DNA kit (Zymo Research, USA). The following program was used ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA from filters from the two North Sea datasets were extracted using the Quick-DNA miniprep kit (Zymo, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Then the two samples were purified by ZYMO Oligo Clean & Concentrator spin columns ...
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bioRxiv - Cell Biology 2023Quote: MAGIC YKO was constructed with a two-step transformation using the Frozen-EZ Yeast Transformation II Kit (T2001, Zymo Research) following the microscale protocol in 96-well format ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... the adaptor-ligated MspI fragments were bisulfite-treated for two consecutive rounds using Zymo’s EZ DNA Methylation-Gold Kit (Zymo cat. D5005) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Molecular Biology 2019Quote: ... Two rounds of bisulfite conversion of the adapter-ligated DNA were performed using EZ-DNA methylation Gold Kit (D5005-Zymo Research), and used as template for three separate PCR reactions (eight cycles of amplification ...
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bioRxiv - Genetics 2020Quote: ... RNA was isolated using two rounds of chloroform extraction followed by isopropanol precipitation or using the Direct-zol RNA Miniprep Plus kit (Zymo Research).
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bioRxiv - Microbiology 2019Quote: ... Total RNA was isolated using TRIzol and the Direct-zol™ RNA MiniPrep Kit with two times of in-column DNase I treatment according to the manufacturer’s instructions (Zymo Research, R2053). RNA purity and concentration were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... epithelial sheets and hyphae pellets were grinded in liquid N2 and processed as described before (Amorim-Vaz et al., 2015a) combining two phase separations and DirectZol™ RNA MiniPrep kit (Zymo Research). DNase treatment was performed off-column using a DNA-free™ Kit (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... control targeted cells (cells that underwent CRISPR/Cas9-mediated targeting and HDR of an expression cassette at a non-GSH locus) and two independent cultures of untargeted cells using Direct-zolTm RNA Miniprep kit (Zymo Research, ZYR.R2052). 1μg of RNA was converted into cDNA with Superscript IV Vilo MM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA and DNA/RNA hybrid isolation was performed in accordance with the manufacturer’s protocol using the ZR-Duet DNA/RNA MiniPrep Plus (ZYMO Research (www.zymoresearch.com ZR-Duet ™ DNA/RNA MiniPrep Plus catalog number D7003 ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from each of the two technical replicates of sorted CFSElow CD4 T cells using Quick-RNA Microprep kit (Zymo Research, Irvine, CA, USA). Without measuring the resulted RNA concentration ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified two times over silica columns (Zymo Research) and quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... PCR products were purified two times over silica columns (Zymo Research) and quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... two samples of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) were added as mock community positive sequencing controls ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified two times over silica columns (Zymo research) and DNA concentration was quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified two times over silica columns (Zymo Research) and the DNA concentration was measured by qPCR using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Genomics 2023Quote: ... Sixty homologous recombination reaction systems were prepared and DNA purification kit (ZYMO ; d4013), and the purified product was transformed into Thermo Fisher Scientific (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... two types of commercially available 897 bp synthetic DNAs (Zymo Research, D4505) were used where all cytosines were modified to 5-mC or unmodified ...
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bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
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bioRxiv - Genetics 2019Quote: ... These final PCR products were purified two times over silica columns (Zymo Research) and quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Two positive sequencing controls (ZymoBiomics Microbial Community DNA Standards; Zymo Research, Irvine, California, USA) and two negative sequencing controls (sterile water ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for two hours followed by agarose gel purification (Zymo Research, USA) and overnight ligation at 4°C (T4 DNA ligase ...
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bioRxiv - Cell Biology 2023Quote: ... all 384 wells were pooled and purified with two DCC-5 columns (Zymo Research). Libraries were sequenced on a NovaSeq6000 system (Illumina ...
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bioRxiv - Genomics 2021Quote: ... gDNA from two plates were pooled and concentrated using DNA Clean & Concentrator-25 (D4033, Zymo) to obtain high enough input and concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... were collected and preserved in two different DNA/RNA Shield™ Fecal Collection tubes (Zymo Research, USA) and stored at 4 °C until further analysis ...
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bioRxiv - Microbiology 2022Quote: ... and two community controls (ZymoBIOMICS Microbial Community DNA Standard and ZymoBIOMICS Microbial Community DNA Standard II (Log Distribution) (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Escherichia coli, Strain 83972 (BEI Resources, Manassas, VA, USA) and two mock community products (Zymo Research, Irvine, CA, USA) were used for validation of the ssUMI pipeline ...
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bioRxiv - Synthetic Biology 2020Quote: ... Mycelium was disrupted with a FastPrep FP120 system (Qbiogene) and total RNA was isolated using the Direct-zol RNA MiniPrep Kit (Zymo Research). For cDNA synthesis ...