Labshake search
Citations for Zymo Research :
1 - 50 of 71 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... containing 2.5 mg/mL Zymolyase (Zymo Research #E1005), 10 mM Sodium phosphate ...
-
bioRxiv - Genomics 2021Quote: ... containing 600 µL of 2x DNA/RNA shield (Zymo Research). gentleMACS M tubes were then placed on the gentleMACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Systems Biology 2023Quote: ... We also included seven negative controls containing only Shield™ (Zymo Research) in the extractions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Tetrads were re-suspended in water containing zymolyase (Zymo research, 0.025 u/μl), incubated at 37°C for 45 sec ...
-
bioRxiv - Microbiology 2020Quote: ... 0.5% Triton X-100 in PBS) containing 5–10 U DNase I (Zymo Research) for 30 min at room temperature ...
-
bioRxiv - Genomics 2021Quote: Cells were FACS sorted into 384-plate wells containing 2 µl lysis buffer (Zymo M-digestion buffer also containing proteinase K) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and submerged in digest solution containing 1X Digest Buffer (Zymo Research, Cat#: D3050-1-5) and supplemented with 1mg/mL Proteinase K (Zymo Research ...
-
bioRxiv - Microbiology 2021Quote: ... 140µl of supernatant was collected in tubes containing 140µl of DNA/RNA Shield (Zymo Research) for SARS-CoV-2 inactivation ...
-
bioRxiv - Immunology 2022Quote: ... containing 1% BME and total RNA purified using the Quick-RNA Microprep kit (Zymo Research). All resulting RNA was used as an input for complementary DNA synthesis using the SMART-Seq v4 kit (Takara Bio ...
-
bioRxiv - Microbiology 2022Quote: ... we included serial dilutions of a mock community containing both bacterial and fungal taxa (i.e., Zymo Research ZymoBIOMICS Mock Community Standard I ...
-
bioRxiv - Molecular Biology 2020Quote: ... The mixture of the RNA containing supernantant and 1 volume of isopropanol was filtered by Zymo-Spin™ ICG Column (Zymo) ...
-
bioRxiv - Immunology 2022Quote: ... Sixty to one hundred twenty milligrams were transferred to lysis tubes containing bashing beads (Zymo Research) and 1 mL TRIzol (Thermo Fisher) ...
-
bioRxiv - Microbiology 2023Quote: ... or placed in 1.5ml cryovials containing 750 ul Xpedition™ RNA/DNA Shield (Zymo Research Products) and stored at ambient temperature before transportation to the laboratory where they were subsequently stored at -20°C until DNA extraction22 ...
-
bioRxiv - Systems Biology 2024Quote: ... padi containing all five developmental stages using Direct-zol RNA Microprep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... stool samples were collected at endpoint and stored in Eppendorf tubes containing DNA/RNA shield (Zymo Research). Samples were sent in dry ice to CosmoID for 16S sequencing and inference of microbiome abundance.
-
bioRxiv - Neuroscience 2022Quote: ... The aqueous phase containing RNA was then further purified using the RNA Clean & Concentrator kit (Zymo, R1013). RNA-seq libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian (Takara ...
-
bioRxiv - Bioengineering 2022Quote: ... Cryofilm pieces containing the tissues were submerged in digest solution consisting of 1X Digestion Buffer (Zymo Research) and 1 mg/ml Proteinase K (Zymo Research ...
-
bioRxiv - Genomics 2023Quote: ... 2 µl adapter filling mixture containing 0.375 mM 5-Methylcytosine dNTP Mix (Zymo Research, cat. no. D1030), 0.75 µl of Thermopol Reaction buffer (New England Biolabs ...
-
bioRxiv - Genomics 2023Quote: ... Samples and tissues were collected into cryovials containing 0.5 mL of DNA/RNA Shield (Zymo R1200 125) or viral transport media (VTM) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Swabs were then placed in 1mL Matrix 2D Screw Tubes containing 400uL of DNA/RNA Shield (Zymo). The tip of the swab was broken off so that only the swab tip was stored in the Matrix 2D Screw Tube ...
-
bioRxiv - Genetics 2023Quote: ... the aqueous phase containing RNA was put through the RNA Clean & Concentrator-5 kit (Zymo Research R1013) with DNase I digestion to remove genomic DNA contamination.
-
bioRxiv - Immunology 2024Quote: ... lung and nasal turbinate tissues were collected in an Omni Bead Ruptor tube containing Tri reagent (Zymo). Subsequently ...
-
bioRxiv - Immunology 2021Quote: ... containing 1% pME and total RNA was isolated using the Quick-RNA MicroPrep Kit (Zymo Research, 11-328M). All subsequent RNA was used for reverse transcription using random hexamer and oligo dT primers with SuperScriptll (ThermoFisher ...
-
bioRxiv - Microbiology 2019Quote: ... 0.5% [w/v] SDS) and transferred into a chilled tube containing zirconia beads (ZYMO Research, Irvine, CA, USA). Primary extractions of RNA were performed with acidic phenol:chloroform (5:1 ...
-
bioRxiv - Immunology 2021Quote: ... Plasmids containing TCR libraries were purified using a ZymoPURE II Plasmid Maxiprep Kit (D4202, Zymo Research, Irvine, CA). The TRBC-p2A-TRAL insert was amplified using a KAPA HiFi HotStart PCR Kit (7958897001 ...
-
bioRxiv - Microbiology 2021Quote: ... using the ZR BashingBead lysis matrix containing 0.1 and 0.5 mm beads (ZR-96 BashingBead Lysis Rack, Zymo Research) and the lysis solution provided in the ZymoBIOMICS 96 MagBead DNA Kit (Zymo Research) ...
-
bioRxiv - Plant Biology 2021Quote: Approximately 30 mg of plant tissues were placed in a 1.5 ml Eppendorf LoBind tube containing glass beads (1.7-2.1 mm diameter, Carl Roth, Karlsruhe, Germany) and 100 µl of TRI-Reagent (Zymo Research). The Eppendorf tube was firmly attached to a SILAMAT S5 vibrator (Ivoclar Vivadent ...
-
bioRxiv - Microbiology 2023Quote: ... suspensions were transferred into ZR BashingbeadTM lysis tubes containing a mix of 0.1 mm and 0.5 mm zirconium beads (Zymo Research) and 750 μL lysis buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ribosome pellet was resuspended in a solubilization buffer containing 0.5% SDS and 1mM EDTA and TRIzol before RNA was extracted using the Direct-zol kit (Zymo Research). RNA was separated on a 15% TBE-Urea gel before RNA between 17nt and 34nt were gel extracted ...
-
bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
-
bioRxiv - Biochemistry 2022Quote: ... the protein solutions were applied to a spin column containing Strep-Tactin XT Superflow suspension (Zymo Research, Irvine, CA, USA). After washing the column in a buffer containing 50 mM HEPES-KOH (pH 7.5) ...
-
bioRxiv - Molecular Biology 2023Quote: The PCR products containing the aforementioned various RNA constructs were cleaned using a PCR DNA clean up kit (Zymo Research), and used in Gibson assembly reactions (5x Gibson buffer ...
-
bioRxiv - Microbiology 2023Quote: ... tissue was scraped from each fragment (avoiding the apical tip) and added directly to 2 mL tubes containing 0.5ml DNA/RNA shield (Zymo Research) and Lysing Matrix A (MP Biomedicals ...
-
bioRxiv - Microbiology 2023Quote: ... The cells debris was removed after centrifugation at 15000g for 5 mins and cell supernatant containing the released virions were subjected to 5μl DNase I (Zymo research) for 1 hour to remove remaining untransfected chimeric NL plasmid ...
-
bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Neuroscience 2019Quote: ... fine forceps were used to manually remove each neuron. Identified neurons (Fig. 1) were immediately placed in a cryogenic microcentrifuge tube containing 400 µL lysis buffer (Zymo Research) and stored at −80°C until RNA extraction ...
-
bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
-
bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
-
bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... the dsDNA target was PCR amplified from the plasmid containing the protospacer and purified by DNA Clean & Concentrator-25 (Zymo Research). The reaction was initiated with incubating 1 μM Casπ RNP effectors with 20 nM dsDNA target at 37 °C for 30 min and then quenched with loading buffer (Gel Loading Dye Purple 6X ...
-
bioRxiv - Biochemistry 2023Quote: ... The gel segments containing the DNA inserts of interest were excised and DNA was purified using a Zymoclean Gel DNA Recovery kit (Zymo Research) (Supplementary Table S1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... aqueous phase containing cfRNAs was mixed with 1 volume of ethanol (95-100%) and then added to the Zymo-Spin column (Zymo, R1016) for RNA binding ...
-
bioRxiv - Microbiology 2024Quote: ... three replicates with 6-20 guts were collected in a screw-cap tube containing 300 μl of DNA/RNA Shield (Zymo Research) (details on number of guts obtained for each sample can be found in Supplementary Table S1).
-
bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to a new 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and heated to 98°C for 10 minutes ...
-
bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and shaken in a thermomixer at 98°C for 10 minutes (15s on/30s off) ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
-
bioRxiv - Genomics 2022Quote: ... separating 22 or 10 (control wells) single nuclei per well in a 96-well plate containing 2.5 μL of M-digestion buffer (Zymo, Cat. D5020-9), 0.25 μL of Proteinase K (Zymo ...
-
bioRxiv - Systems Biology 2022Quote: Oropharyngeal swabs and stool samples were collected in collection tubes containing DNA/RNA shield (Zymo Research Cat# R1107-E and Cat# R1101) and frozen at -80C until further analysis was performed ...