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Citations for Zymo Research :
101 - 150 of 262 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... DNA fragments were excised from gel and purified using Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Plasmids were isolated from E ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite conversion of the methylated adapter-ligated DNA fragments was achieved using the EZ Methylation-Gold kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... and then these DNA fragments were treated twice with bisulfite using the EZ DNA Methylation-GoldTM kit (Zymo Research). The resulting single-stranded DNA fragments were PCR-amplified using KAPA HiFi HotStart Uracil + ReadyMix (2x) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were isolated and concentrated using the Zymoclean Large Fragment DNS Recovery Kit (Zymo Research, Irvine, CA, USA) and DNA Clean & Concentrator-5 (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... The un-crosslinked sample was ethanol precipitated and purified using the large fragment DNA recovery kit (Zymo Research, D4046). Religated-biotinylated restriction fragments were pulled down using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Adaptor-ligated fragments were then bisulfite-treated using the EZ DNA Methylation-Lightning™ Kit (Zymo Research, Cat# D5459). Preparative-scale PCR (16 cycles ...
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bioRxiv - Genomics 2023Quote: ... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research, CA. USA). The final library was subjected to paired-end sequencing using an Illumina NovaSeq 6000 sequencer with a read length of 150 bp.
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bioRxiv - Biochemistry 2023Quote: ... The fragments were purified from agarose gels over silica using agarose dissolving buffer (Zymoclean Gel DNA Recovery, Zymo Research). The library fragments were cloned into pRSF accepter vector described above ...
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bioRxiv - Microbiology 2022Quote: ... coli TOP10 cells were propagated in LB medium (ampicillin, 100 µg/ml) and used for recombinant plasmid DNA isolation (Zypy Plasmid Miniprep, Zymo Research). Prior to plasmid transformation to expression host E ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Genomics 2020Quote: ... 1ng of each library constructed was used in a polymerase chain reaction with primers flanking the allele and the barcode to generate fragments which were subsequently gel verified and extracted using Zymo gel extraction kit (Zymo). 25ng of the purified product was subjected to self-ligation at 16 °C overnight in a total volume of 50uls and column purified using Qiagen (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... the transposed DNA fragments were harvested and cleaned up with Zymo DNA Clean and Concentrator-5 kit (D4011, Zymo Research). The libraries were indexed and amplified with Illumina i5/i7 indexing primers (FC-121-1011 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were purified from the reaction using a RNA Clean & Concentrator(tm)-5 column (Zymo Research, Cat. n° R1013). The resulting RNA fragments were ligated to different adaptors (listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... all PCR reactions were DpnI treated to digest the methylated plasmid template DNA before the PCR fragments were purified with the DNA Clean & Concentrator Kit (Zymo). To construct pMRE-Himar-13X ...
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bioRxiv - Synthetic Biology 2022Quote: ... and fragment mixes for homology dependent assembly (Figure 5) were cleaned and concentrated with DNA Clean & Concentrator-5 columns (Zymo), and DNA was eluted with 10 μL molecular grade water ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid-backbone and insert fragments were isolated from gels with the Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine CA). DNA concentrations were measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... tissue was scraped from each fragment (avoiding the apical tip) and added directly to 2 mL tubes containing 0.5ml DNA/RNA shield (Zymo Research) and Lysing Matrix A (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... The fragmentation was assessed on an agarose gel and fragments between 150 and 350 bp were gel extracted (Zymo #4007). End repair was performed following the manufacturer’s instructions and purified (NEB #E6050) ...
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bioRxiv - Cell Biology 2023Quote: ... the band with the expected size of a fully assembled fragment was purified (Zymoclean Gel DNA Recovery Kit, Zymo Research) and cloned in pJet1.3 as described above ...
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bioRxiv - Genomics 2021Quote: ... Fragments between 400-700 bp were excised from the gel and recovered using the ZymoClean Gel DNA Recovery Kit (Zymo Research). The final libraries were quantified using the Qubit dsDNA BR Kit (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... the adaptor-ligated MspI fragments were bisulfite-treated for two consecutive rounds using Zymo’s EZ DNA Methylation-Gold Kit (Zymo cat. D5005) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... DNA fragments used for probe library generation were amplified by PCR and recovered by DNA Cleanup kit (D4014, Zymo research, U.S.A.). The amplified probe library could serve as templates for Tn5-FISH probes ...
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bioRxiv - Microbiology 2021Quote: ... the PCR products were run on an agarose gel and the DNA fragments size 325 – 625 bp were extracted using Zymoclean Gel DNA Recovery kit (Zymo Research). The DNA libraries were quantified utilizing Qubit dsDNA RB Assay kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The DNA was subjected to size selection and purification with a gel extraction kit (Large Fragment DNA Recovery Kit; ZYMO RESEARCH). The sequencing of purified genome was performed using PromethION at GeneBay ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel slice corresponding to 70-90 nucleotides was excised from the PAGE gel and the RNA fragments were recovered using ZR small-RNA PAGE Recovery Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
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bioRxiv - Microbiology 2021Quote: ... The digested fragments were purified using the DNA Clean & Concentrator-5 Kit and the Zymoclean Gel DNA Recovery Kit (Zymo Research) respectively ...
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bioRxiv - Genetics 2022Quote: ... Resulting cDNA was separated on a 4% agarose gel and the 160–190 nt long fragments were extracted and gel purified using the Zymoclean Gel DNA Recovery Kit (D4008, ZYMO RESEARCH). Small RNA Libraries were sequenced in triplicates on a NovaSeq S1 SR100 mode (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... the DNA fragments were treated twice with bisulfite using an EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). The libraries were then prepared according to the Illumina standard DNA methylation analysis protocol.
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bioRxiv - Microbiology 2023Quote: ... the same PCR fragment used for Sanger sequencing was purified using a ZymocleanTM Gel DNA Recovery Kit (Zymo Research, cat# D4002). DNA (∼1 µg ...
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bioRxiv - Plant Biology 2023Quote: ... and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research, Irvine, CA. USA). The final library was sequenced using a 2 × 150 bp paired-end protocol on the NovaSeq 6000 system (Illumina).
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Molecular Biology 2020Quote: ... and frozen at −80°C in 500 ul TRI-Reagent (Zymo). Frozen worms were thawed on ice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We ran the libraries out on a 1.5% agarose gel and extracted 300-800 bp fragments using a Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, USA). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was cut to purify fragments from 200 bp to 600 bp using Zymoclean Gel DNA Recovery Kit (Zymo Research, D4007). The purified DNA libraries were measured by the Qubit HS ds DNA Assay Kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Plant Biology 2019Quote: ... 70 ng of Covaris sheared gDNA (average fragment size = 200 bp) was bisulfite converted using the EZ DNA Methylation-Gold Kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified samples were run on a 2% agarose gel and fragments of the correct size were purified using ADB Agarose Dissolving Buffer (Zymo D4001-1-100) and UPrep Micro Spin Columns (Genesee Scientific 88– 343) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and eluted in 10 μL prewarmed 65°C provided elution buffer (Zymo). Six μL of each were then combined and sequenced off the U2 region via Illumina amplicon sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and eluted in 10 μL prewarmed 65°C provided elution buffer (Zymo). Six μL of each were then combined and sequenced off the U2 region by Illumina sequencing on one lane of HiSeq4000 SR50 at the QB3 Genomics Sequencing Laboratory at UC Berkeley.
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bioRxiv - Molecular Biology 2023Quote: ... for 30min at 30°C followed by column purification (Zymo research, R1016). The following reverse transcription (RT ...
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bioRxiv - Genomics 2021Quote: ... which were then grown overnight at 37 °C and subsequently purified (Zymo, D4200). For each step ...
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bioRxiv - Genomics 2019Quote: 200 ng of mouse E14 genomic DNA sheared to 15 kb fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research), or an EpiTect Bisulfite Kit (QIAGEN ...
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bioRxiv - Plant Biology 2023Quote: The bisulfite conversion of sonicated genomic DNA fragments was carried out based on the instructions provided in the EZ DNA-methylation lightning Kit (Zymo Research Corp., Irvine, CA, USA). The libraries were made using the Accel-NGS Methyl-Seq DNA library kit (SWIFT Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... at 30°C for 30 min and removed by RNA Clean & Concentrator (Zymo Research). Reverse transcription reactions including the ligated RNA above ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL CircLigase) for 12 hours at 60°C and subsequently purified by Zymo RNA Clean & Concentrator 5 columns (100 μL sample ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for two hours followed by agarose gel purification (Zymo Research, USA) and overnight ligation at 4°C (T4 DNA ligase ...
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bioRxiv - Molecular Biology 2022Quote: ... Digest of the vector (1 h, 37 C) was purified from agarose gel (Zymo Research). We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... #C3040) at 30°C and purified using the Zyppy Plasmid Miniprep kit (Zymo Research, #D4036) or ZymoPURE II Plasmid Midiprep kit (Zymo Research ...