Labshake search
Citations for Zymo Research :
501 - 550 of 566 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... DNA obtained from anti-OVA hits were then cut out from agarose gels and amplicons from heavy and light chains were purified separately by gel extraction (Zymoclean Gel DNA Recovery Kit, Zymo Research).
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... was added to 200 μl of homogenized tissue (see DNA extraction) and processed using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DnaseI digestion ...
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bioRxiv - Immunology 2023Quote: ... A second RNA extraction using TRIZOL was carried out and completed by RNA purification with Direct-zol RNA Miniprep (Zymo Research). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified by phenol-chloroform extraction and the aqueous fraction was further cleaned using DNA Clean & Concentrator-5 with Zymo-Spin Columns (Zymo Research). Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... we extracted total RNA from each line (5 wt lines and 5 +0A lines) using the Zymo Direct-Zol RNA extraction kit (Zymo Research). This RNA was immediately reverse transcribed using Superscript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: Isolates for sequencing were cherrypicked using an Opentrons OT-2 Liquid Handler and DNA extraction was then performed using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) on a KingFisher Flex instrument (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... A 200 mg soil sample from each falcon tube was taken for DNA extraction using the Zymo Quick DNA Fecal/Soil Microbe MiniPrep Kit (Zymo Research). Before sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Modifications to the published method are the following: 1) extraction of RNA prior to cDNA reverse transcription with the Zymo Direct-zol-96 RNA isolation kit (Zymo, #R0256) 2 ...
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Stored bacterial pellets were used for RNA extraction using the Quick-RNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: For short read Illumina sequencing DNA extractions were carried out with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, UK), with the addition for S ...
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified by gel extraction from a 1.5 % low-melt agarose gel using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Samples were quantified using the Qubit dsDNA HS assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Nucleic acid samples were extracted directly from oro-pharyngeal swab samples using a Direct-zol™ RNA MiniPrep Plus extraction kit (Zymo Research) and in full compliance to the manufacturers’ recommendations ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... DNA-digestion and RNA-extraction was performed using the Zymo-Spin IC Column from Direct-zol RNA MicroPrep kit (Zymo Research, R2060) per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Total DNA extractions were performed for tea broth and pellicle samples using Quick-DNA Fecal/soil Microbe Miniprep kit (ZYMO Research Corp.) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using TRIzol-based (Fisher: 15596026) phenol-chloroform extraction and purified with RNA Clean and Concentrator-5 spin columns (Zymo research: R2050). RNA concentration and quality was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons at 15 DIV were left untreated or treated for 48 h with 2 μM TTX and then processed for total RNA extraction using the kit Direct-zol™ RNA MicroPrep (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Tissue RNA extraction was performed using the Direct-zol RNA miniprep plus kit with DNAase treatment per package insert (Zymo, Irvine, California). SIV and CD4 primers for RT PCR and amplification conditions were as previously published [28] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of fractions 10-16 were pooled and RNA was by acidic phenol chloroform extraction followed by column purification (Zymo Research, R1013) as described above ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Microbiology 2022Quote: ... yielding optimal in vitro probiotics characteristics were subjected to bacterial DNA extraction as per kit instructions (Zymo BIOMICS DNA Miniprep kit, USA). The 16S rDNA PCR was performed in a 10 µL reaction containing 1X KAPA HiFi-Hotstart Ready mix (KAPA ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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bioRxiv - Genomics 2023Quote: ... These samples were used as the starting material for an RNA extraction using the Quick-RNA Viral Kit (Zymo, Cat no: R1034). The final elution was collected in 15 μl of nuclease-free water.
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 10 trunk fragments per biological replicate using the standard Trizol RNA extraction protocol with high salt precipitation and clean-up on Zymo RNA columns (Zymo R1013/R1017). RNA (1 μg ...
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bioRxiv - Immunology 2023Quote: ... RNA was isolated and purified using a TRIzol extraction procedure according to the manufacturer’s instructions (Zymo Kit direct-Zol RNA kit #R2050). Briefly ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Microbiology 2023Quote: Viral RNA extraction from nasal washes of infected ferrets was done using the Quick-RNA Viral 96 Kit (Zymo, Cat. No. R1040) using 160 µL sample volume ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were frozen in liquid nitrogen and mechanically homogenized before total RNA extraction was performed using the Quick-RNA™ Microprep Kit (Zymo Research). 200ng from each sample were transferred to GenTegra-RNA tubes (Syntezza Bioscience ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was conducted using a CTAB protocol combined with the Zymo DNA clean and concentrator kit (Zymo Research Corp., Orange, CA, USA) as reported previously [71] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Microbial DNA extractions were performed in batches (stored on ice) immediately after intestinal dissections with the Zymobiomics DNA Miniprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Developmental Biology 2020Quote: RNA was isolated directly from the TRIzol muscle samples using a 96 well plate extraction kit (Direct-zol™-96 RNA, Zymo Research, #R2054): after thawing to room temperature in 1,5 ml Eppendorf tubes ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminant before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). 4-8 tubules were collected for each sample.
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bioRxiv - Microbiology 2020Quote: ... 75μl supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated using phenol-chloroform extraction and then purified and concentrated using ethanol precipitation and washing on a silica column (RNA Clean & Concentrator-5, Zymo Research, Irvine, CA). Total RNA samples were reverse transcribed (ThermoFisher SuperScirpt IV Vilo) ...
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bioRxiv - Microbiology 2021Quote: ... 50 µl of apical wash was used for the extraction of nuclease digestion-resistant viral RNA using the Quick-RNA Viral kit (#R1035; Zymo Research, Irvine, CA), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The total DNA from each bacterium was extracted using a DNA extraction kit (ZR soil microbe DNA MiniPrep™ kit (Zymo Research, USA) according to the manufacturer’s instructions [35] ...
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bioRxiv - Microbiology 2021Quote: ... Only 1/4 of the resulting sludge (450 µl) was used for gut genomic DNA extraction with Quick-DNA Fecal and Soil Microbe Kit (Zymo Research, California, U.S.A). The 16S rRNA gene amplification and libraries preparation for Illumina MiSeq platform sequencing were carried out according to Alberoni et al. ...
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bioRxiv - Genomics 2019Quote: ... Brain tissue samples were used for DNA and RNA extraction using the ZR-Duet DNA/RNA MiniPrep Plus kit (Zymo Genesee 11-385P). All procedures were approved by the UC Davis IACUC committee ...
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bioRxiv - Genomics 2021Quote: ... Microbial DNA was extracted and purified after return to the lab with a Zymo Soil/Fecal DNA extraction kit (Zymo Research, Irvine, CA). Quantification of DNA was performed with a Qubit 3.0 fluorimeter (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... samples were processed for RNA extraction and purification using the Zymo Direct-zol™ RNA Mini Prep kit (Zymo Research, Irvine, CA, USA) per manufacturer instructions ...
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bioRxiv - Pathology 2022Quote: Approximately 10 mg of each tissue was macerated in extraction buffer and RNA extracted using the ZYMO Research Quick DNA/RNA Pathogen Miniprep kit (ZYMO Research, Irvine, California) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2022Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminants before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). Four to eight tubules were collected for each sample for a total length of 2 – 4 mm.
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bioRxiv - Bioengineering 2023Quote: ... and total genomic DNA was extracted from algal cell pellets (∼50-100 mg) with a Zymo Quick-DNA fungal/bacterial extraction kit (Zymo Research group, USA) according to the manufacturer’s protocol ...