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Citations for Zymo Research :
701 - 750 of 835 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from MV4;11 Cas9 + GeCKOv2 cells collected before and following sustained C6-treatment using the Quick-gDNA MidiPrep Kit (Zymo Research) per manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2021Quote: ... reporter assay was carried out as described above in presence of Nsp1 or Nsp2 followed by harvesting of cells in Trizol (Zymo Research). The RNA was isolated using Direct-zol RNA miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from the sorted or the control cells either using the Quick-DNA Mini Kit or the Midi Plus Kit (Zymo Research). The integrated LentiCRISPRv2 vector sgRNA sequences were amplified through three rounds of PCR using the Taq DNA Polymerase S (high specificity ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...
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bioRxiv - Neuroscience 2019Quote: ... The gDNA of the sorted cells (both N2A and NSCs) was extracted with the Genomic DNA(tm) - Tissue MiniPrep kit (Zymo Research) following the protocol of the manufacturer for cell suspensions ...
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bioRxiv - Microbiology 2019Quote: Genomic extraction was preformed on 105 – 106 cells from the in vitro evolution experiments using the Quick-DNA Miniprep Plus Kit (Zymo Research). The gDNA was then diluted to a range between 100-200ng/5μl in TE buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Sorted cells were collected in PBS, centrifuged (2000 rpm, 5 min) and RNA extracted using the Quick RNA Mini Prep (Zymo Research) according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: Total cellular RNA from BMDM cell lines or tissues was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2019Quote: ... 1.5-mL aliquots of cell culture were used as input in minipreps with the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: RNA extraction of whole lung pieces or purified cells was performed using the Quick-RNA Miniprep or Quick-RNA Microprep (Zymo Research), respectively ...
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bioRxiv - Cell Biology 2021Quote: mIMCD3 wild type and Jade mutant cells were washed with PBS and RNA extraction was performed with the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer’s instructions including a DNase1 treatment step ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: Dim and lit cells were sorted into 500 µl TriZol and RNA was extracted with DirectZol RNA purification kit (Zymo Research) with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from cultured cells using BIO TRI RNA reagent (Bio-lab) and Direct-zol RNA MiniPrep kit (Zymo research), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... The cells and medium were then collected and subjected to RNA extraction using the Direct-zol RNA MiniPrep kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Experiments to determine whether CxNV1 has DNA forms were initiated by extracting RNA from CT cells using Direct-Zol RNA MicroPrep (Zymo Research), and separately ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Cells were infected with 1 MOI of TB40/EGFP and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0-72 hpi ...
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bioRxiv - Genomics 2021Quote: ... The remaining cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from cultured NSC-34 cells or primary motor neurons was isolated using DirectZole RNA Purification Kit (Zymo Research, R2052), and reverse transcribed using qScript cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus preparation or supernatants of virus-infected cells using a viral RNA extraction kit (Quick-RNA Viral Kit, R1035, Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were sorted by FACS as described above and RNA were extracted by using Quick- RNA Microprep Kit (Zymo Research, R1050) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The extracted DNA (100-1000 ng) from anal cells was bisulfite conversion by using EZ kit Gold Bisulfite Conversion Kit (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from cells following CTS or control treatments using the ZR-Duet™ DNA/RNA MiniPrep kit (Zymo D7001). RNA concentration and quality were measured using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...