Labshake search
Citations for Zymo Research :
151 - 200 of 255 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... and supplemented with 1mg/mL Proteinase K (Zymo Research, Cat#: D3001-2-5) for 1 hour at 55°C ...
-
bioRxiv - Immunology 2022Quote: pAd/S1RS09cg proteins were amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
-
bioRxiv - Genomics 2021Quote: Cells were FACS sorted into 384-plate wells containing 2 µl lysis buffer (Zymo M-digestion buffer also containing proteinase K) ...
-
bioRxiv - Genomics 2020Quote: ... run on 2 % agarose gel and extracted using the Zymo Gel Extraction Kit (Zymo) with final elution in 16 uL ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 2×106 cells were stored in 1X DNA/RNA Shield (Zymo Research, U.S.) at -80°C for downstream molecular analysis.
-
bioRxiv - Plant Biology 2023Quote: ... 2 ml of cultures were harvested and Direct-Zol RNA MiniPrep kit (Zymo Research) was used ...
-
bioRxiv - Genomics 2024Quote: ... the annealed oligos were incubated with 10 mM 5-hydroxymethyl-2’-dCTP (Zymo Research), along with dGTP ...
-
bioRxiv - Biochemistry 2023Quote: ... and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research). Both 5’ ends of 1 pmole of the CRISPR leader and array fragments were then labelled with 32P ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from 2 × 106 cells using the Quick-RNA miniprep kit (Zymo) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: RNA was extracted from 2 dpf whole embryos using the Directzol RNA mini kit (Zymo). cDNA was synthesised and amplified using Thermo Fisher SuperScript II Reverse Transcriptase Kit ...
-
bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
-
bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
-
bioRxiv - Genomics 2022Quote: ... The supernatant (300µl) was mixed with 30µl of Proteinase K (Zymo Cat #D3001-2-20) digestion buffer and 15µl of Proteinase K in a new tube using a brief vortex step ...
-
bioRxiv - Genomics 2022Quote: ... then thawed and combined with 2 volumes of DNA Shield (Zymo Research, Irvine, CA, USA). Genomic DNA was extracted using a Quick DNA Miniprep Plus kit (Zymo Research ...
-
bioRxiv - Microbiology 2021Quote: RNA was extracted from CaCo-2 cells using Direct-zol™ RNA Microprep (ZYMO cat: R2062), according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: Plasmids of sorted and unsorted populations where extracted using the Zymo prep kits (Zymo, version 2) with modifications as described previously19 ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the nucleic-acid bound protein isolation was performed using buffers from the Quick-RNA Miniprep Kit (Zymo Research) and the Zymo-Spin IIICG Column (Zymo Research) ...
-
bioRxiv - Cell Biology 2023Quote: ... Endogenous RNA cross-linked to bait protein was recovered from the beads using the QuickRNA Microprep Kit (Zymo Research). Complementary DNA was synthesized using the SuperScript III First-Strand Synthesis kit (Invitrogen ...
-
bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
-
bioRxiv - Genomics 2022Quote: ... for 2 hours at 37C then cleaned using a Zymo DNA Clean & Concentrator column-5 (Zymo #D4013). The cleaned RCA product was digested using NEBNext dsDNA Fragmentase (NEB #M0348 ...
-
bioRxiv - Microbiology 2020Quote: An aliquot of each fraction was then mixed 1:1 with 2× DNA/RNA Shield (Zymo Research) spiked with in vitro transcribed RNA (generated by T7 transcription off a Firefly luciferase (Fluc)-containing plasmid) ...
-
bioRxiv - Biochemistry 2020Quote: ... The released RNA was then cleaned up by adding 2 volumes of RNA Lysis Buffer (Zymo Research) and one volume of 100% ethanol ...
-
bioRxiv - Microbiology 2023Quote: ... and 2) amplification of DNA extracted from a ZymoBIOMICS Microbial Community Standard (Zymo Research, Irvine, CA U.S.A.). Contaminating ASVs were identified through the use of the decontam package 84 ...
-
bioRxiv - Genomics 2023Quote: ... 2 µl adapter filling mixture containing 0.375 mM 5-Methylcytosine dNTP Mix (Zymo Research, cat. no. D1030), 0.75 µl of Thermopol Reaction buffer (New England Biolabs ...
-
bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
-
bioRxiv - Biochemistry 2022Quote: ... the protein solutions were applied to a spin column containing Strep-Tactin XT Superflow suspension (Zymo Research, Irvine, CA, USA). After washing the column in a buffer containing 50 mM HEPES-KOH (pH 7.5) ...
-
bioRxiv - Immunology 2019Quote: ... Total RNA from approximately 2 × 106 cells were isolated using the Direct-Zol RNA isolation kit (Zymo Research). RNAseq libraries were prepared as previously described 70 using KAPA Standed RNA-seq library preparation kit (KAPA Biosystems) ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfections were performed with PEI and 2-5 µg of transfection grade DNA (ZymoPure Plasmid MaxiPrep Kit, Zymo) per dish as described in above ...
-
bioRxiv - Systems Biology 2020Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
-
bioRxiv - Plant Biology 2021Quote: ... 500 pg - 2 µg DNA was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research). Amplicons for sequencing were generated by PCR using degenerate primers (see Supplemental Table 1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 45°C and purified using the ChIP DNA Clean and Concentrator Kit (Zymo Research).
-
bioRxiv - Genomics 2021Quote: ... RNA was isolated from 2 day-differentiated TXΔXicB6 cells using the Direct-Zol RNA Miniprep Kit (Zymo Research). To remove any remaining gDNA ...
-
bioRxiv - Immunology 2022Quote: Genomic DNA from 2×106 NCI-H295R cells was isolated with the Quick-DNA Miniprep Kit (Zymo research). Bisulfite treatment was performed using the EZ DNA Methylathion™ Kit (Zymo Research) ...
-
bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 min at 30Hz and extracted RNA using the Direct-Zol RNA MiniPrep kit (Zymo Research, USA) with on-column DNA digest according to the manufacturer protocol ...
-
bioRxiv - Genomics 2023Quote: ... 2 x 500 ng of DNA was bisulfite modified with the EZ DNA Methylation-Lightning kit (\#D5030, Zymo) and standardized to a concentration of 25 ng/uL BC-DNA ...
-
bioRxiv - Systems Biology 2023Quote: ... We then sequentially added and centrifuged through the column 400 µL DNA/RNA prep buffer (Zymo, #D7010-2), 600 µL DNA/RNA wash buffer (Zymo ...
-
bioRxiv - Systems Biology 2023Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
-
bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
-
bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
-
bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
-
bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... We thawed the cell pellets on ice and then resuspended with 400 µL RNA binding buffer (Zymo, #R1013-2). The mixtures were then transferred to 2 mL screw cap tubes with zirconia beads inside ...
-
bioRxiv - Genomics 2023Quote: ... the resulting poly-A+ RNA fraction (corresponding to 0.7-2% of the starting amount) was then purified again on a Clean & Concentrator-5 Column Kit (Zymo) and eluted in 10µL of molecular-grade water.
-
bioRxiv - Microbiology 2019Quote: ... The filter-associated biofilm was disrupted by adding 1 ml PBS + 0.01% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension was diluted ...
-
bioRxiv - Molecular Biology 2021Quote: ... This library pool was then size selected on a 2% agarose gel by cutting between 150-800 nucleotides and performing gel purification (Zymo). To determine the loading density of the final pooled library ...
-
bioRxiv - Microbiology 2020Quote: The Zymobiomics microbial community DNA standard consisting of 8 bacterial and 2 fungal genomic DNAs (cat. no. D6305) was obtained from Zymo Research (Irvine ...
-
bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
-
bioRxiv - Microbiology 2022Quote: ... Filters were then transferred into a 1.5 ml tube and the filter-associated cells were resuspended by adding 1 ml 1X PBS + 0.05% Triton X-100 detergent and agitating the tubes at high speed for 2 minutes using a Genie Disruptor (Zymo). This suspension as well as the inoculum were diluted ...