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Citations for Zymo Research :
1 - 50 of 1020 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Genetics 2020Quote: ... Tagged DNA was purified with the DNA Clean & Concentrator-5 kit (Zymo Research) eluting with 14 µl EB buffer (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The tagged DNA was purified with the DNA Clean ConcentratorTM5 Kit (Zymo Research). The samples were pre-amplified with the Nextera Index kit and the Nextera DNA Library Prep kit (both from Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The tagged DNA was purified using DNA clean and concentrator kit (Zymo research). Fluorescently labeled DNA was transferred on to Zeta probe membrane (Bio Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Tagged DNA fragments were purified using the Clean & Concentrator-5 Kit (ZYMO, D4014), then subjected to PCR amplification and double-sided bead purification (to remove primer dimers and larger than 1,000 bp fragments ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water ...
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bioRxiv - Microbiology 2020Quote: An aliquot of each fraction was then mixed 1:1 with 2× DNA/RNA Shield (Zymo Research) spiked with in vitro transcribed RNA (generated by T7 transcription off a Firefly luciferase (Fluc)-containing plasmid) ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmid DNA was purified using the ZR Bac DNA Miniprep Kit (Zymo Research) and correctness of the transposed sequence was analyzed by PCR using pUC/M13 primers ...
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bioRxiv - Genomics 2021Quote: Cells were FACS sorted into 384-plate wells containing 2 µl lysis buffer (Zymo M-digestion buffer also containing proteinase K) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2×106 cells were stored in 1X DNA/RNA Shield (Zymo Research, U.S.) at -80°C for downstream molecular analysis.
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant reporter constructs were isolated using the ZymoPURE™ Plasmid Miniprep Kit (Zymo Research, #D4210), following the manufacturer’s protocol with the following minor modifications ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from 2 × 106 cells using the Quick-RNA miniprep kit (Zymo) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... cucullus vegetative cells (300,000 cells) and 2-week-old mature cysts (3,000,000 cells) using the Direct-zol RNA purification system (Zymo Research Crop., California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: PCR testing for SARS-CoV-2 was carried out on respiratory specimens mixed 1:1 in DNA/RNA Shield (Zymo Inc) using an in-house Clinical Laboratory Improvement Amendments (CLIA ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were directly lysed in 1 ml of Trizol (R2050-1, Zymo Research) and subjected to interface cleanup as described below.
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from CaCo-2 cells using Direct-zol™ RNA Microprep (ZYMO cat: R2062), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
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bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and MagBead DNA/RNA Wash 2 buffer (#R2130-2, Zymo Research). After magnetically pelleted ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...
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bioRxiv - Immunology 2019Quote: ... Total RNA from approximately 2 × 106 cells were isolated using the Direct-Zol RNA isolation kit (Zymo Research). RNAseq libraries were prepared as previously described 70 using KAPA Standed RNA-seq library preparation kit (KAPA Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... RNA was isolated from 2 day-differentiated TXΔXicB6 cells using the Direct-Zol RNA Miniprep Kit (Zymo Research). To remove any remaining gDNA ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 2×106 NCI-H295R cells was isolated with the Quick-DNA Miniprep Kit (Zymo research). Bisulfite treatment was performed using the EZ DNA Methylathion™ Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... We thawed the cell pellets on ice and then resuspended with 400 µL RNA binding buffer (Zymo, #R1013-2). The mixtures were then transferred to 2 mL screw cap tubes with zirconia beads inside ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in Tri-reagent (R2050-1-50, Zymo Research) and total RNA was extracted using Quick-RNA Miniprep kit (R1055 ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in Tri-reagent (R2050-1-50, Zymo Research) and RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: Cells were harvested using Tri reagent (Zymo, Cat#R2050-1-200) and total mRNA was extracted using the RNA Clean & Concentrator™-5 (Zymo ...
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... coli TOP10 cells were propagated in LB medium (ampicillin, 100 µg/ml) and used for recombinant plasmid DNA isolation (Zypy Plasmid Miniprep, Zymo Research). Prior to plasmid transformation to expression host E ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Genomics 2022Quote: ... RNA was extracted from ~1×106 cells (Zymo Direct-zol RNA microPrep). Total RNA was reverse transcribed using a capture sequence-specific reverse transcription primer along with an oligo(dT)V30 primer (400ng RNA ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed using RNA Lysis Buffer (Zymo Research, R1060-1-50), and RNA was isolated according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed with TRI Reagent® (Zymo Research, R2050-1-50) and RNA was extracted using Direct-zol™ RNA Miniprep Plus (Zymo Research ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA (gDNA) was isolated from a saturated overnight culture (approximately 2 x 108 cells) using YeaStar Genomic DNA Kit (Zymo Research). Genomic DNA (125 µL at 15 ng µL−1 in ultrapure water ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the cells were pelleted and resuspended at 1x106 cells per 300 ul of RNA lysis buffer (Zymo, #R1060-1-100). Cells were pipette mixed and frozen at -80 until RNA isolation was performed ...