Labshake search
Citations for Zymo Research :
401 - 450 of 6397 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the resulting poly-A+ RNA fraction (corresponding to 0.7-2% of the starting amount) was then purified again on a Clean & Concentrator-5 Column Kit (Zymo) and eluted in 10µL of molecular-grade water.
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Neuroscience 2022Quote: ... total cellular RNA using ∼1×106 cells from one well of a six-well plate was isolated using TRIzol reagent followed by Direct-zol RNA MiniPrep Plus kit purification (Zymo Research # R2070). RNA was quantified using Qubit RNA HS Assay kit and cDNA synthesised from ∼2µg of RNA using SuperScript IV VILO Master Mix kit following gDNA removal with ezDNase ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from individual eGFP+ cell lines was extracted from 96 well-plates using Quick-DNA Universal 96 Kit (Zymo Research, D4071) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets collected from 48-well plates were lysed and total RNAs were extracted using the Direct-zolTM RNA Miniprep kit (Zymo Research R2053) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The enriched RNA and input total RNA from 5% of lysate were purified using Direct-zol RNA Purification Kit (Zymo). Target genes in input total RNA and enriched RNA were quantified by qRT-PCR using SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... The reaction was incubated 10 minutes at 55°C followed by purification with DNA Clean & Concentrator-5 kit (Zymo Research) and elution in 21 µL of water ...
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bioRxiv - Cancer Biology 2020Quote: ... the transposed DNA fragments were harvested and cleaned up with Zymo DNA Clean and Concentrator-5 kit (D4011, Zymo Research). The libraries were indexed and amplified with Illumina i5/i7 indexing primers (FC-121-1011 ...
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bioRxiv - Plant Biology 2021Quote: ... RNA samples were digested with DNase I and purified using RNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembly is cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 3 μl Gel Loading Dye ...
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bioRxiv - Bioengineering 2022Quote: ... The IVT reaction was assembled based on the manufacturer’s recommendations and the sgRNA was purified using an RNA Clean & Concentrator-5 kit (Zymo Research). RNA concentration was measured using a NanoDrop (ThermoFisher)
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bioRxiv - Plant Biology 2021Quote: ... The RNA was then purified and concentrated using the RNA Clean Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). cDNA libraries were prepared using a mix of 50 ng RNA and 0.5 ng Spike-in RNA Variant Control Mix E2 (Lexogen ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated using the PerfectPure RNA Cultured Cell kit (5 PRIME) or Quick-RNA™ MiniPrep (Zymo Research). One microgram of the total RNA was reverse-transcribed into cDNA with M-MLV (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... per manufacturer protocol and reaction products were purified with the Zymo Clean & Concentrator - 5 kit (Zymo Research #R1016, Irvine CA). RNA concentration post labeling was measured with the Qubit RNA HS kit (ThermoFisher #Q32852) ...
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bioRxiv - Genetics 2020Quote: ... viral RNA was prepared from 200 µl of clarified virus stocks (3.000 rpm, 5 min) with the Quick-RNA Viral Kit (Zymo research) and eluted in 14 µl RNase free H20 ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from lumbar DRGs 2-5 following the manufacturer’s protocol with a Direct-zol RNA Miniprep Plus kit (Zymo R2071). RNA libraries were prepared and sequenced by the UTSW Microarray core facility on an Illumina NextSeq SE-75 sequencer at 40 million reads/sample ...
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bioRxiv - Microbiology 2020Quote: ... RNA was cleaned and concentrated to 10 μl using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA), as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... P7 and E18.5 was directly dissolved in RNA isolation buffer and RNA was isolated using the Quick-RNA micro prep kit (Zymo). Library preparation was done using the NEB Next® Ultra™ RNA Library Prep Kit ...
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bioRxiv - Cell Biology 2022Quote: ... all wells were pooled to purify DNA products using the Zymo DNA Clean & Concentrator-5 kit (Zymo, Cat #: 11-303) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from ∼6x105-7x105 MRC-5 infected fibroblasts using Zymo Duet RNA/DNA isolation Kit (Zymo Research). Viral genomes were quantitated by quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... PCR purification and plasmid isolation was done using the DNA Clean & Concentrator-5 and Zyppy Plasmid Miniprep Kits from Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted and purified using Trizol reagent and RNA Clean & Concentrator-5 kit (Zymo Research cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... decorated cDNA from multiple samples were pooled together and purified using the DNA Clean & concentrator-5 Kit (Zymo research, #D4014). After elution with 20 µl of nuclease free water ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: RNA was isolated directly from the TRIzol muscle samples using a 96 well plate extraction kit (Direct-zol™-96 RNA, Zymo Research, #R2054): after thawing to room temperature in 1,5 ml Eppendorf tubes ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were plated randomly across 96-well plates and provided to the Emory University Integrated Genomics Core for bisulfite modification using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA), and subsequent assessment of genome-wide DNAm using the Illumina MethylationEPIC Beadarray (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Microbiology 2019Quote: ... The enriched circular DNA samples were then purified by DNA Clean & ConcentratorTM-5 kit according to manufacturer’s instructions (Zymo Research, USA). Elution was done with 10 µl of 10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Microbiology 2019Quote: ... The assembled DNA was then cleaned and concentrated using Zymo Clean and Concentrator-5 kit (SKU D4013 Zymo Research, Irvine, CA), quantified by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was isolated from cell pellets in 5 batches (each batch contained one of each of the 5 strains) using the ZR Fungal/Bacterial RNA mini-prep kit including the DNase I on-column digestion step (Zymo Research). Each cell pellet was resuspended in lysis buffer and transferred to screw-capped tubes containing glass beads and the cells were broken open using a mini-beadbeater (Biospec Products ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Developmental Biology 2019Quote: The cDNA of all single cells was collected together and further concentrated with the DNA Clean and Concentrator−5 kit (Zymo Research). The Exonuclease I (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... 5 heads of each genotype were pooled and total RNA extracted using the Direct-zol RNA Microprep kit (Zymo Research, R2060) and the quality determined using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Products were checked on an agarose gel and cleaned-up through a PCR-clean up kit (DNA Clean and Concentrator-5, Zymo, D4013), followed by a secondary PCR to add illumina indexes (SFig ...
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bioRxiv - Developmental Biology 2022Quote: ... Gel band within 100-250bp was cut and purified using Zymoclean Gel DNA recovery kit/ DNA Clean & Concentrator-5 (Zymo Research). If needed ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by a 5-day incubation period before total RNA was collected by Direct-zol RNA MiniPrep Plus kit (Zymo Research). 69 RNASeq profiles ...