Labshake search
Citations for Zymo Research :
101 - 143 of 143 citations for Prestained Protein Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... see below) to which we added 50 μl of ZymoBIOMICS Microbial Community Standard Cells (Zymo Research Corp., Irvine, CA, USA). Mock sap samples were then processed as indicated above for the PowerPlant DNA extraction protocol ...
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bioRxiv - Genomics 2023Quote: ... High molecular weight DNA extracted from whole blood by standard protocols was bisulfite converted with the EZ-96 DNA Methylation-Gold Kit (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... from weight-normalized contents from the distal small intestine and proximal colon after addition of Low Abundance Microbiota Standard (Zymo) and extraction using the Zymo DNA Mini kit (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Genomics 2021Quote: ... The yeast DNA samples were collected by thawing frozen yeast and following the standard procedure of a Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research).
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction reagent only controls were included for each batch of DNA extractions along with ZymoBIOMICS Microbial Community Standards (Zymo Research) and E ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted from the fecal and intestinal samples and from two negative extraction controls and two ZymoBIOMICS Microbial Community Standard positive extraction controls (D6300 Zymo Research) with the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Microbiology 2022Quote: ... we added negative DNA extractions and PCR controls from each time point and mock communities (ZymoBIOMICS microbial community standard, Zymo, Irvine, CA) to each library for additional quality control and inference.
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bioRxiv - Microbiology 2019Quote: ... and a mock microbial community DNA (8 bacterial + 2 yeasts species mixed in defined proportions) (ZymoBIOMICS Microbial Community DNA standard, Zymo Research, USA) were included in the experiments ...
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bioRxiv - Plant Biology 2019Quote: ... to several others we added a mock microbial community as a positive control (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We extracted DNA using the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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bioRxiv - Genomics 2023Quote: Reads were aligned to the 16S genes extracted from the complete reference genomes of the bacteria present in the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research D6305) using minimap2 ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 10 trunk fragments per biological replicate using the standard Trizol RNA extraction protocol with high salt precipitation and clean-up on Zymo RNA columns (Zymo R1013/R1017). RNA (1 μg ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Microbiology 2021Quote: ... buffer without any added fecal material) and two microbial mock communities as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, California, USA).
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bioRxiv - Microbiology 2022Quote: ... Reagent blanks were included as negative controls and cells from a microbial community of known composition were included as positive controls (ZymoBiomics Microbial Community Standards; Zymo Research, Irvine, CA). The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... we also produced ten separate V3-V4 16S rRNA gene amplicon libraries from the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA) using the 2-Step library preparation method ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer specificity was tested by performing ddPCR in the presence of an excess of eukaryotic (fish) or prokaryotic [ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA)] DNA ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was also performed on three no-sample added (negative) and three ZymoBIOMICS Microbial Community Standard (positive) controls (Zymo Research, Irvine, CA, USA). DNA from an additional eight GWSS had been previously extracted using the Blood and Tissue kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
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bioRxiv - Neuroscience 2020Quote: ... Protein degradation was performed by adding Proteinase K (Zymo) and Qiagen Protease (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... a negative control to account for microbial contaminants present in DNA extraction kits or introduced during the extraction (Eisenhofer et al., 2019) and a microbial community standard (Zymo Research, cat. no. D6300, Irving, CA). The DNA concentration was quantified with Qubit 3 using the high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP-DNA was prepared using ZymoMag Protein A beads (Zymo Research). Input samples were also prepared using 10% of chromatin samples ...
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bioRxiv - Immunology 2022Quote: pAd/S1RS09cg proteins were amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research). Both 5’ ends of 1 pmole of the CRISPR leader and array fragments were then labelled with 32P ...
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bioRxiv - Neuroscience 2022Quote: ... Then the nucleic-acid bound protein isolation was performed using buffers from the Quick-RNA Miniprep Kit (Zymo Research) and the Zymo-Spin IIICG Column (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous RNA cross-linked to bait protein was recovered from the beads using the QuickRNA Microprep Kit (Zymo Research). Complementary DNA was synthesized using the SuperScript III First-Strand Synthesis kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... DNA was captured on protein A/G agarose beads and purified using the ChIP DNA Clean & Concentrator kit (Zymo) and used for qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Biochemistry 2022Quote: ... the protein solutions were applied to a spin column containing Strep-Tactin XT Superflow suspension (Zymo Research, Irvine, CA, USA). After washing the column in a buffer containing 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Plant Biology 2024Quote: ... was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Bioengineering 2022Quote: Yeast competent cells were transformed with polycistronic constructs containing fluorescent proteins using the Frozen-EZ yeast transformation II kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...